氮源添加对重金属污染土壤氨氧化微生物的影响

doi:10.16258/j.cnki.1674-5906.2024.005.010

生态环境学报 ›› 2024, Vol. 33 ›› Issue (5): 771-780.DOI: 10.16258/j.cnki.1674-5906.2024.005.010

• 研究论文【环境科学】 • 上一篇下一篇

氮源添加对重金属污染土壤氨氧化微生物的影响

于方明¹^,², 袁月¹^,², 曾梦¹^,², 唐舒婷³, 李艺¹^,²^,^*()

1.广西师范大学/珍稀濒危动植物生态与环境保护教育部重点实验室，广西桂林 541004
2.广西师范大学环境与资源学院，广西桂林541004
3.中山大学环境科学与工程学院，广东广州 510006

收稿日期:2024-02-11 出版日期:2024-05-18 发布日期:2024-06-27
通讯作者: * 李艺。E-mail: liyi412@mailbox.gxnu.edu.cn
作者简介:于方明（1975年生），男，教授，博士，主要从事环境污染生物修复研究。
基金资助:
国家自然科学基金项目(42367006);国家自然科学基金项目(42367001);广西自然科学基金项目(2021GXNSFAA220024)

Variations on the Ammonia Oxidizers under Different Nitrogen Fertilization Regimes in Heavy Metal-contaminated Soil

YU Fangming¹^,², YUAN Yue¹^,², ZENG Meng¹^,², TANG Shuting³, LI Yi¹^,²^,^*()

1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education/Guangxi Normal University, Guilin 541004, P. R. China
2. College of Environment and Resources, Guangxi Normal University, Guilin 541004, P. R. China
3. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, P. R. China

Received:2024-02-11 Online:2024-05-18 Published:2024-06-27

摘要/Abstract

摘要：

矿业活动导致矿区土壤养分流失、土地退化，土壤生态系统敏感且脆弱，恢复过程复杂。其中土壤氮素缺乏是限制该生态系统恢复的主要因子之一。为探讨矿区土壤氮素恢复机制，以广西柳州泗顶矿区重金属污染土壤为研究对象，通过土壤培养实验，采用高通量测序结合荧光定量PCR技术，系统研究了氯化铵（38.114 g∙m⁻¹∙a⁻¹(高浓度)、9.54 g∙m⁻¹∙a⁻¹(低浓度)）和尿素（21.43 g∙m⁻¹∙a⁻¹（高浓度）、5.36 g∙m⁻¹∙a⁻¹（低浓度））在不同添加频次（添加12次∙年⁻¹（高频率）和2次∙年⁻¹（低频率））下，对土壤氨氧化古菌（AOA）和氨氧化细菌（AOB）丰度、多样性和群落组成的影响。结果表明，各施氮模式下，amoA-AOB基因丰度显著高于amoA-AOA基因丰度，其丰度范围为 (1.56×10⁷±0.01×10⁷)－(3.58×10⁷±0.03×10⁷) copies·g⁻¹（氯化铵）和 (5.31×10⁷±0.02×10⁷)－(14.85×10⁷±0.04×10⁷) copies·g⁻¹（尿素）。AOA群落的ACE、Shannon和Simpson指数均值分别是AOB群落的13.2、1.41和0.627倍，AOA的α-多样性更容易受到不同施氮处理的影响。AOA在门水平上的优势菌门为奇古菌门（Thaumarchaeota）和泉古菌门（Crenarchaeota）；在属水平上的优势菌属为亚硝基球菌属（Nitrososphaera）。AOB在门水平上的优势菌门为变形菌门（Proteobacteria）；在属水平上的优势菌属为亚硝化螺菌属（Nitrosospira）和亚硝化弧菌属（Nitrosovibrio）。相关性分析表明土壤有效磷是影响amoA-AOB基因丰度的关键因素（p<0.01）。冗余分析表明，微生物量氮是引起AOA群落组成改变的主要因子；而土壤脲酶活性是引起AOB群落组成改变的主要因子。添加氯化铵和尿素使土壤氨氧化潜势和总硝化潜势增加，分别是对照的1.15－3.03倍和2.15－8.55倍，AOB主导土壤中的氨氧化和硝化过程。研究为明确矿区土壤氮循环变化规律，重建矿区土壤氮库提供了理论依据。

关键词: 氯化铵, 尿素, 氨氧化细菌, 氨氧化古菌, amoA基因丰度

Abstract:

Mining activities lead to nutrient loss and land degradation in mining areas, where soil ecosystems are sensitive and fragile, making the restoration process complex and difficult. Among these limiting factors, nitrogen deficiency is a major obstacle to ecosystem recovery. To explore the mechanisms of nitrogen restoration in the soils of mining areas, this study focused on heavy metal-contaminated soils from the Siding Mining Area of Liuzhou, Guangxi. By conducting soil cultivation experiments and using high-throughput sequencing in combination with quantitative PCR technology, this study systematically investigated the effects of different application regimes of ammonium chloride (38.114 g∙m⁻¹∙a⁻¹ (high concentration), 9.54 g∙m⁻¹∙a⁻¹ (low concentration)), and urea (21.43 g∙m⁻¹∙a⁻¹ (high concentration), 5.36 g∙m⁻¹∙a⁻¹ (low concentration)) with 12 times∙a⁻¹ (high abundance) and 2 times∙a⁻¹ (low abundance) added nitrogen on the abundance, diversity, and community composition of soil ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). The results showed that the abundance of amoA-AOB gene was significantly higher than the abundance of amoA-AOA gene under different nitrogen application regimes, ranging from 1.56±0.01 to 3.58±0.03×10⁷ copies·g⁻¹ (ammonium chloride) and 5.31±0.02 to 14.85±0.04×10⁷ copies·g⁻¹ (urea). The average ACE, Shannon, and Simpson indices of the AOA community were 13.2, 1.41, and 0.627 times higher, respectively, than those of the AOB community, suggesting that the α-diversity of AOA was more susceptible to different nitrogen treatments. The dominant phyla in AOA were Crenarchaeota, and Thaumarchaeota and the dominant genus was Nitrososphaera. For AOB, the dominant phylum was Proteobacteria and the dominant genera were Nitrospira and Nitrosovibrio. Correlation analysis showed that available phosphorus in the soil was a key factor affecting the abundance of amoA-AOB gene. Redundancy analysis showed that nitrogen in microbial biomass was the main factor causing changes in AOA community composition, whereas urease activity in the soil was the main factor affecting changes in AOB community composition. The addition of ammonium chloride and urea increased the potential ammonia oxidation and total nitrification potential of the soil by 1.15- to 3.03-fold and 2.15- to 8.55-fold, respectively, compared with the control, with AOB dominating the ammonia oxidation and nitrification processes in the soil. This study provides a theoretical basis for clarifying the patterns of changes in the nitrogen cycle in the soils of mining areas and rebuilding the soil nitrogen pool.

Key words: ammonium chloride, urea, ammonia-oxidizing bacteria, ammonia-oxidizing archaea, amoA gene abundance

中图分类号:

X172

于方明, 袁月, 曾梦, 唐舒婷, 李艺. 氮源添加对重金属污染土壤氨氧化微生物的影响[J]. 生态环境学报, 2024, 33(5): 771-780.

YU Fangming, YUAN Yue, ZENG Meng, TANG Shuting, LI Yi. Variations on the Ammonia Oxidizers under Different Nitrogen Fertilization Regimes in Heavy Metal-contaminated Soil[J]. Ecology and Environment, 2024, 33(5): 771-780.

图/表 7

参考文献 43

[1]	BAI X, HU X J, LIU J J, et al., 2022. Ammonia oxidizing bacteria dominate soil nitrification under different fertilization regimes in black soils of northeast China[J]. European Journal of Soil Biology, 111: 103410.
[2]	CHAUDHARY A K, PANDIT R, BURTON M, 2021. Effect of socioeconomic and institutional factors and sustainable land management practices on soil fertility in smallholder farms in the Mahottari District, Nepal[J]. Land Degradation & Development, 33(2): 269-281.
[3]	DAI X L, GUO Q K, SONG D L, et al., 2021. Long-term mineral fertilizer substitution by organic fertilizer and the effect on the abundance and community structure of ammonia-oxidizing archaea and bacteria in paddy soil of south China[J]. European Journal of Soil Biology, 103(10): 103288.
[4]	DINCĂ L C, GRENNI P, ONET C, et al., 2022. Fertilization and soil microbial community: A review[J]. Applied Sciences, 12(3): 1198.
[5]	DOBSON A P, BRADSHAW A D, BAKER A J M, 1997. Hopes for the future: restoration ecology and conservation biology[J]. Sicence, 277(5325): 515-522.
[6]	DUAN P P, ZHANG Q Q, XIONG Z Q, 2020. Temperature decouples ammonia and nitrite oxidation in greenhouse vegetable soils[J]. Science of The Total Environment, 733: 139391.
[7]	FAN D W, WANG S Y, GUO Y H, et al., 2021. Cd induced biphasic response in soil alkaline phosphatase and changed soil bacterial community composition: The role of background Cd contamination and time as additional factors[J]. Science of the Total Environment, 757: 143771.
[8]	GAN C D, CUI S F, WU Z Z, et al., 2022. Multiple heavy metal distribution and microbial community characteristics of vanadium-titanium magnetite tailing profiles under different management modes[J]. Journal of Hazardous Materials, 429: 128032.
[9]	GONZALEZ-ALCARAZ M N, VAN GESTEL C A M, 2016. Toxicity of a metal(loid)-polluted agricultural soil to Enchytraeus crypticus changes under a global warming perspective: Variations in air temperature and soil moisture content[J]. Science of the Total Environment, 573: 203-211.
[10]	GREENFIELD L M, PUISSANT J, JONES D L, 2021. Synthesis of methods used to assess soil protease activity[J]. Soil Biology and Biochemistry, 158: 108277.
[11]	LI Y Y, CHAPMAN S J, NICOL G W, et al., 2018. Nitrification and nitrifiers in acidic soils[J]. Soil Biology and Biochemistry, 116: 290-301.
[12]	LIMA H S, CAVALCANTE J P C M, SILVA L C F, et al., 2022. Structure and putative function of a soil microbial community impacted by the deposition of tailings and subsequent revegetation after the rupture of the Fundao Dam[J]. Land Degradation & Development, 33(8): 1235-1248.
[13]	LIU H Y, QIN S Y, LI Y, et al., 2023. Comammox Nitrospira and AOB communities are more sensitive than AOA community to different fertilization strategies in a fluvo-aquic soil[J]. Agriculture, Ecosystems & Environment, 342: 108224.
[14]	LIU L, LI D Z, SUN Y M, et al., 2021. Pattern of soil extracellular enzyme activities along a tidal wetland with mosaic vegetation distributions in Chongming Island, China[J]. Journal of Cleaner Production, 315(1): 127991.
[15]	MARTENS-HABBENA W, BERUBE P M, URAKAWA H, et al., 2009. Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria[J]. Nature, 461: 976-982.
[16]	MARX M C, WOOD M, JARVIS S C, 2011. A microplate fluorimetric assay for the study of enzyme diversity in soils[J]. Soil Biology and Biochemistry, 33(12-13): 1633-1640.
[17]	MENG F B, YANG X D, DUAN L C, et al., 2019. Influence of pH, electrical conductivity and ageing on the extractability of benzo[a]pyrene in two contrasting soils[J]. Science of the Total Environment, 690: 647-653.
[18]	NEMATI K, ABU BAKAR N K, ABAS M R, et al., 2011. Speciation of heavy metals by modified BCR sequential extraction procedure in different depths of sediments from Sungai Buloh, Selangor, Malaysia[J]. Journal of Hazardous Materials, 192(1): 402-410. DOI PMID
[19]	OUYANG Y, NORTON J M, STARK J M, et al., 2016. Ammonia-oxidizing bacteria are more responsive than archaea to nitrogen source in an agricultural soil[J]. Soil Biology and Biochemistry, 96: 4-15.
[20]	QIAO L K, LIU X X, ZHANG S, et al., 2021. Distribution of the microbial community and antibiotic resistance genes in farmland surrounding gold tailings: A metagenomics approach[J]. Science of the Total Environment, 779: 146502.
[21]	SIMON J, KLOTZ M G, 2013. Diversity and evolution of bioenergetic systems involved in microbial nitrogen compound transformations[J]. Biochimica et Biophysica Acta, 1827: 114-135. DOI PMID
[22]	SINGH A N, KUMAR A, 2022. Comparative soil restoration potential of exotic and native woody plantations on coal mine spoil in a dry tropical environment of India: A case study[J]. Land Degradation & Development, 33(12): 1971-1984.
[23]	SUN R B, GUO X S, WANG D Z, et al., 2015. Effects of long-term application of chemical and organic fertilizers on the abundance of microbial communities involved in the nitrogen cycle[J]. Applied Soil Ecology, 95: 171-178.
[24]	TANG S T, RAO Y, HUANG S L, et al., 2023. Impact of environmental factors on the ammonia-oxidizing and denitrifying microbial community and functional genes along soil profiles from different ecologically degraded areas in the Siding mine[J]. Journal of Environmental Management, 326(Part A): 116641.
[25]	TAYLOR A E, ZEGLIN L H, WANZEK T A, et al., 2012. Dynamics of ammonia-oxidizing archaea and bacteria populations and contributions to soil nitrification potentials[J]. The ISME Journal, 6(11): 2024-2032.
[26]	VERHAMME D T, PROSSER J I, NICOL G W, 2011. Ammonia concentration determines differential growth of ammonia-oxidising archaea and bacteria in soil microcosms[J]. The ISME Journal, 5(6): 1067-1071.
[27]	WANG J C, WANG J L, RHODE G, et al., 2019. Adaptive responses of comammox Nitrospira and canonical ammonia oxidizers to long-term fertilizations: implications for the relative contributions of different ammonia oxidizers to soil nitrogen cycling[J]. Science of the Total Environment, 668: 224-233.
[28]	WANG J C, ZHANG L, LU Q, et al., 2014. Ammonia oxidizer abundance in paddy soil profile with different fertilizer regimes[J]. Applied Soil Ecology, 84: 38-44.
[29]	WESSÉN E, NYBERG K, JANSSON J K, et al., 2010. Responses of bacterial and archaeal ammonia oxidizers to soil organic and fertilizer amendments under long-term management[J]. Applied Soil Ecology, 45(3): 193-200.
[30]	YANG K N, LUO S W, HU L G, et al., 2020. Responses of soil ammonia-oxidizing bacteria and archaea diversity to N, P and NP fertilization: Relationships with soil environmental variables and plant community diversity[J]. Soil Biology and Biochemistry, 145: 107795.
[31]	YANG W H, HU H, RU M, et al., 2013. Changes of microbial properties in (near-) rhizosphere soils after phytoextraction by Sedum alfredii H: A rhizobox approach with an artificial Cd-contaminated soil[J]. Applied Soil Ecology, 72: 14-21.
[32]	YING J Y, LI X X, WANG N N, et al., 2017. Contrasting effects of nitrogen forms and soil pH on ammonia oxidizing microorganisms and their responses to long-term nitrogen fertilization in a typical steppe ecosystem[J]. Soil Biology and Biochemistry, 107: 10-18.
[33]	YU G R, JIA Y L, HE N P, et al., 2019. Stabilization of atmospheric nitrogen deposition in China over the past decade[J]. Nature Geoscience, 12: 424-429.
[34]	ZHANG L M, HU H W, SHEN J P, et al., 2012. Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils[J]. The ISME Journal, 6: 1032-1045.
[35]	ZHANG L X, GUAN Y T, JIANG S C, 2021. Investigations of soil autotrophic ammonia oxidizers in farmlands through genetics and big data analysis[J]. Science of The Total Environment, 777: 146091.
[36]	ZHANG N L, WAN S Q, LI L H, et al., 2008. Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China[J]. Plant and Soil, 311: 19-28.
[37]	ZHENG H J, MEI Z L, 2013. Key processes and microbial mechanisms of soil nitrogen transformation[J]. Microbiology China, 40: 98-108.
[38]	ZOU W X, LANG M, ZHANG L, et al., 2022. Ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea dominate nitrification in a nitrogen-fertilized calcareous soil[J]. Science of The Total Environment, 811: 151402.
[39]	郭俊杰, 朱晨, 刘文波, 等, 2021. 不同施肥模式对土壤氮循环功能微生物的影响[J]. 植物营养与肥料学报, 27(5): 751-759.
	GUO J J, ZHU C, LIU W B, et al., 2021. Effects of different fertilization managements on functional microorganisms involved in nitrogen cycle[J]. Journal of Plant Nutrition and Fertilizers, 27(5): 751-759.
[40]	金兰淑, 郑佳, 徐慧, 等, 2009. 施氮及灌溉方式对玉米地土壤硝化潜势及微生物量碳的影响[J]. 水土保持学报, 23(4): 218-220.
	JIN L S, ZHENG J, XU H, et al., 2009. Effect of different nitrogen condition for soil microbial biomass carbon and potential nitrification rate of corn[J]. Journal of Soil and Water Conservation, 23(4): 218-220.
[41]	李晨华, 贾仲君, 唐立松, 等, 2012. 不同施肥模式对绿洲农田土壤微生物群落丰度与酶活性的影响[J]. 土壤学报, 49(3): 567-574.
	LI C H, JIA Z J, TANG L S, et al., 2012. Effect of model of fertilization on microbial abundance and enzyme activity in oasis farmland soil[J]. Acta Pedologica Sinica, 49(3): 567-574.
[42]	鲁如坤, 1999. 土壤农业化学分析方法[M]. 中国农业科技出版社.
	LU R K, 1999. Soil agrochemical analysis methods[M]. China Agricultural Science and Technology Press.
[43]	赵晶, 冯文强, 秦鱼生, 等, 2010. 不同氮磷钾肥对土壤pH和镉有效性的影响[J]. 土壤学报, 47(5): 953-961.
	ZHAO J, FENG W Q, QIN Y S, et al., 2010. Effects of application of nitrogen, phosphorus and potassium fertilizers on soil pH and cadmium availability[J]. Acta Pedologica Sinica, 47(5): 953-961.

时间 (年-月)	处理	TNP/ (mg·kg⁻¹·d⁻¹)	NP-AOB/ (mg·kg⁻¹·d⁻¹)	NP-AOA/ (mg·kg⁻¹·d⁻¹)	NP-AOB占TNP比例/%
2021-09	对照	1.58±0.01b	0.06±0.00fg	0.13±0.01b	34.2
	AC-HH	0.96±0.01h	0.13±0.01bc	0.02±0.02d	56.8
	AC-HL	1.15±0.01e	0.05±0.02g	0.08±0.02c	63.0
	AC-LH	1.47±0.02c	0.16±0.01b	0.02±0.01d	57.9
	AC-LL	1.34±0.01d	0.20±0.00a	0.21±0.01a	67.2
	对照	1.58±0.01b	0.06±0.00fg	0.13±0.01b	34.2
	U-HH	1.87±0.01a	0.14±0.01bc	0.09±0.00bc	28.2
	U-HL	0.96±0.01g	0.08±0.01ef	0.07±0.02a	43.9
	U-LH	1.06±0.01f	0.12±0.01cd	0.11±0.01bc	56.8
	U-LL	0.88±0.01i	0.10±0.01de	0.01±0.01d	50.7
2021-12	对照	44.8±0.02d	0.48±0.01i	0.88±0.01f	6.42
	AC-HH	40.6±0.01e	1.83±0.01b	1.45±0.01c	16.4
	AC-HL	26.1±0.03i	1.27±0.02f	0.83±0.02g	20.8
	AC-LH	71.0±0.01a	1.72±0.01c	1.42±0.01c	9.44
	AC-LL	29.8±0.01h	0.90±0.01h	0.47±0.01h	9.76
	对照	44.8±0.02d	0.48±0.01i	0.88±0.01f	16.6
	U-HH	46.3±0.01c	3.39±0.01a	2.62±0.01a	6.42
	U-HL	32.2±0.03g	1.48±0.01e	1.06±0.02e	27.1
	U-LH	48.8±0.02b	1.66±0.01d	1.62±0.01b	18.8
	U-LL	34.7±0.01f	1.13±0.01g	1.34±0.00d	14.7
2022-06	对照	10.2±0.00i	0.10±0.01i	0.04±0.01i	0.96
	AC-HH	27.1±0.00f	1.43±0.00e	0.17±0.00h	5.28
	AC-HL	22.3±0.01g	0.78±0.01g	0.37±0.01f	3.52
	AC-LH	27.7±0.01d	1.97±0.00d	0.28±0.00g	7.12
	AC-LL	22.0±0.01h	0.34±0.01h	0.61±0.01e	1.56
	对照	10.2±0.00i	0.10±0.01i	0.04±0.01i	0.96
	U-HH	63.7±0.01c	4.78±0.00b	6.88±0.06a	7.50
	U-HL	45.3±0.01b	3.34±0.01c	2.74±0.01c	7.36
	U-LH	87.4±0.01a	5.12±0.01a	3.59±0.01b	5.85
	U-LL	27.2±0.00e	1.03±0.00f	2.29±0.01d	3.80

时间 (年-月)	处理	TNP/ (mg·kg⁻¹·d⁻¹)	NP-AOB/ (mg·kg⁻¹·d⁻¹)	NP-AOA/ (mg·kg⁻¹·d⁻¹)	NP-AOB占TNP比例/%
2021-09	对照	1.58±0.01b	0.06±0.00fg	0.13±0.01b	34.2
	AC-HH	0.96±0.01h	0.13±0.01bc	0.02±0.02d	56.8
	AC-HL	1.15±0.01e	0.05±0.02g	0.08±0.02c	63.0
	AC-LH	1.47±0.02c	0.16±0.01b	0.02±0.01d	57.9
	AC-LL	1.34±0.01d	0.20±0.00a	0.21±0.01a	67.2
	对照	1.58±0.01b	0.06±0.00fg	0.13±0.01b	34.2
	U-HH	1.87±0.01a	0.14±0.01bc	0.09±0.00bc	28.2
	U-HL	0.96±0.01g	0.08±0.01ef	0.07±0.02a	43.9
	U-LH	1.06±0.01f	0.12±0.01cd	0.11±0.01bc	56.8
	U-LL	0.88±0.01i	0.10±0.01de	0.01±0.01d	50.7
2021-12	对照	44.8±0.02d	0.48±0.01i	0.88±0.01f	6.42
	AC-HH	40.6±0.01e	1.83±0.01b	1.45±0.01c	16.4
	AC-HL	26.1±0.03i	1.27±0.02f	0.83±0.02g	20.8
	AC-LH	71.0±0.01a	1.72±0.01c	1.42±0.01c	9.44
	AC-LL	29.8±0.01h	0.90±0.01h	0.47±0.01h	9.76
	对照	44.8±0.02d	0.48±0.01i	0.88±0.01f	16.6
	U-HH	46.3±0.01c	3.39±0.01a	2.62±0.01a	6.42
	U-HL	32.2±0.03g	1.48±0.01e	1.06±0.02e	27.1
	U-LH	48.8±0.02b	1.66±0.01d	1.62±0.01b	18.8
	U-LL	34.7±0.01f	1.13±0.01g	1.34±0.00d	14.7
2022-06	对照	10.2±0.00i	0.10±0.01i	0.04±0.01i	0.96
	AC-HH	27.1±0.00f	1.43±0.00e	0.17±0.00h	5.28
	AC-HL	22.3±0.01g	0.78±0.01g	0.37±0.01f	3.52
	AC-LH	27.7±0.01d	1.97±0.00d	0.28±0.00g	7.12
	AC-LL	22.0±0.01h	0.34±0.01h	0.61±0.01e	1.56
	对照	10.2±0.00i	0.10±0.01i	0.04±0.01i	0.96
	U-HH	63.7±0.01c	4.78±0.00b	6.88±0.06a	7.50
	U-HL	45.3±0.01b	3.34±0.01c	2.74±0.01c	7.36
	U-LH	87.4±0.01a	5.12±0.01a	3.59±0.01b	5.85
	U-LL	27.2±0.00e	1.03±0.00f	2.29±0.01d	3.80

土壤指标	amoA-AOA基因丰度	amoA-AOB基因丰度
土壤含水率	0.226	−0.453*
pH	−0.010	−0.187
电导率	−0.327	−0.113
有效态Cd	−0.217	0.227
有效态Pb	−0.190	−0.460*
总氮	−0.573**	−0.020
微生物氮	0.277	0.849**
总碳	0.052	0.283
微生物碳	−0.004	0.283
总磷	−0.176	0.537**
有效磷	0.344	0.651**
纤维素梅	0.240	0.647**
蔗糖酶	0.110	0.322
淀粉酶	0.104	0.379
脲酶	0.135	0.694**
蛋白酶	0.185	0.469*
酸性磷酸酶	0.093	0.519**
PAO	0.368	0.983**

土壤指标	amoA-AOA基因丰度	amoA-AOB基因丰度
土壤含水率	0.226	−0.453*
pH	−0.010	−0.187
电导率	−0.327	−0.113
有效态Cd	−0.217	0.227
有效态Pb	−0.190	−0.460*
总氮	−0.573**	−0.020
微生物氮	0.277	0.849**
总碳	0.052	0.283
微生物碳	−0.004	0.283
总磷	−0.176	0.537**
有效磷	0.344	0.651**
纤维素梅	0.240	0.647**
蔗糖酶	0.110	0.322
淀粉酶	0.104	0.379
脲酶	0.135	0.694**
蛋白酶	0.185	0.469*
酸性磷酸酶	0.093	0.519**
PAO	0.368	0.983**

氮源添加对重金属污染土壤氨氧化微生物的影响

Variations on the Ammonia Oxidizers under Different Nitrogen Fertilization Regimes in Heavy Metal-contaminated Soil

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