生态环境学报 ›› 2022, Vol. 31 ›› Issue (7): 1383-1392.DOI: 10.16258/j.cnki.1674-5906.2022.07.010
李程程(), 张子蕤, 宋晓萱, 孔娟娟, 韩阳, 阮亚男*
收稿日期:
2022-03-22
出版日期:
2022-07-18
发布日期:
2022-08-31
通讯作者:
*阮亚男,女,副教授。作者简介:
李程程(1987年生),女,讲师,博士研究生,研究方向为种群生态学、生态适应、恢复生态学。E-mail: lichengcheng@lnu.edu.cn
基金资助:
LI Chengcheng(), ZHANG Zirui, SONG Xiaoxuan, KONG Juanjuan, HAN Yang, RUAN Yanan*
Received:
2022-03-22
Online:
2022-07-18
Published:
2022-08-31
摘要:
在全球变化背景下,近地层臭氧(O3)浓度不断升高,将成为影响未来农业生产的重要因素。以大豆(Glycine max)为研究材料,采用OTCs方法,设置3个处理梯度(CK、80 nmol∙mol-1、200 nmol∙mol-1)臭氧熏蒸试验,试验进行9 d,在熏蒸结束后的72 h内(恢复期)分别采集各处理分枝期大豆,分析叶片膜脂过氧化程度、抗氧化酶活性及相关基因表达的变化情况,以及在大豆完熟期测定生长及结实性状。结果表明,(1)低浓度(80 nmol∙mol-1)臭氧处理显著提高了完熟期大豆豆荚数和单株种子粒数,但种子单株粒质量小于对照。(2)低浓度臭氧熏蒸使分枝期大豆叶片抗坏血酸(AsA)、谷胱甘肽(GSH)含量升高,超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性上升,72 h内的恢复期间,抗氧化酶活性升高,表现出氧化应激反应。(3)高浓度(200 nmol∙mol-1)熏蒸处理使分枝期大豆叶片活性氧水平显著升高,过氧化氢酶(CAT)、APX、GR、脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)活性下降,CAT1、APX、GR表达下调,MDA含量升高。在恢复期,SOD、CAT、APX、GR活性升高,相关酶基因表达上调。该研究表明低浓度(80 nmol∙mol-1)臭氧急性胁迫后,在恢复期大豆能够通过升高抗氧化酶活性及基因表达调控适应臭氧浓度升高带来的氧化胁迫,并在生殖上提高豆荚及单株粒数,保证亲本投入。但急性高浓度(200 nmol∙mol-1)胁迫将对大豆的生长和成株的生殖造成损害,且伤害难以恢复。
中图分类号:
李程程, 张子蕤, 宋晓萱, 孔娟娟, 韩阳, 阮亚男. 臭氧胁迫对大豆抗氧化代谢与生殖生长的影响[J]. 生态环境学报, 2022, 31(7): 1383-1392.
LI Chengcheng, ZHANG Zirui, SONG Xiaoxuan, KONG Juanjuan, HAN Yang, RUAN Yanan. Effects of Ozone Stress on Antioxidant Metabolism and Reproductive Growth of Soybean[J]. Ecology and Environment, 2022, 31(7): 1383-1392.
数量性状 Traits | x(O3)/(nmol∙mol-1) | ||
---|---|---|---|
CK | 80 | 200 | |
叶面积 Leaf area/cm2 | 51.28±1.76a | 51.70±1.08a | 37.94±2.20b |
株高 Plant height/cm | 90.13±6.51a | 89.25±4.37a | 81.00±2.17b |
地上生物量 (干质量) Aboveground/g | 13.83±1.95a | 15.25±1.66a | 10.241±0.57b |
地下生物量 Underground/g | 2.33±0.58a | 1.46±0.17b | 0.85±0.10b |
豆荚数 Pod | 29.25±0.48b | 34.50±2.22a | 25.25±2.87b |
单株粒数 Seed | 65.75±4.40b | 80.50±5.56a | 59.00±4.8b |
单株粒重 Individual grain weight/g | 11.31±1.18a | 12.31±1.76a | 8.35±1.06b |
百粒重 Hundred-gain weight/g | 16.98±0.52a | 16.17±0.62b | 14.35±0.30c |
表1 不同浓度臭氧处理下大豆主要数量性状的多重比较
Table 1 Multiple comparison of main quantitative traits of soybean with different ozone concentrations
数量性状 Traits | x(O3)/(nmol∙mol-1) | ||
---|---|---|---|
CK | 80 | 200 | |
叶面积 Leaf area/cm2 | 51.28±1.76a | 51.70±1.08a | 37.94±2.20b |
株高 Plant height/cm | 90.13±6.51a | 89.25±4.37a | 81.00±2.17b |
地上生物量 (干质量) Aboveground/g | 13.83±1.95a | 15.25±1.66a | 10.241±0.57b |
地下生物量 Underground/g | 2.33±0.58a | 1.46±0.17b | 0.85±0.10b |
豆荚数 Pod | 29.25±0.48b | 34.50±2.22a | 25.25±2.87b |
单株粒数 Seed | 65.75±4.40b | 80.50±5.56a | 59.00±4.8b |
单株粒重 Individual grain weight/g | 11.31±1.18a | 12.31±1.76a | 8.35±1.06b |
百粒重 Hundred-gain weight/g | 16.98±0.52a | 16.17±0.62b | 14.35±0.30c |
图1 不同浓度臭氧处理结束后对大豆叶片O2∙-产生速率、H2O2含量的影响 CK:自然环境臭氧摩尔分数为 (40.16±8.22) nmol∙mol-1;80:臭氧摩尔分数为80 nmol∙mol-1;200:臭氧摩尔分数为200 nmol∙mol-1,下同
Figure 1 Effects of elevated ozone pretreatment to O2∙-production rate, H2O2 content in soybean leaves CK: ozone mole fraction: 40.16±8.22 nmol∙mol-1; 80: ozone mole fraction: 80 nmol∙mol-1; 200: ozone mole fraction: 200 nmol∙mol-1, The same below
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