Effects of Different Fertilization Regimes on Community Structure Diversity of CO2-assimilating Bacteria in Maize Field of Fluvo-aquic Soil in North China

doi:10.16258/j.cnki.1674-5906.2022.04.009

Ecology and Environment ›› 2022, Vol. 31 ›› Issue (4): 715-722.DOI: 10.16258/j.cnki.1674-5906.2022.04.009

• Research Articles • Previous Articles Next Articles

Effects of Different Fertilization Regimes on Community Structure Diversity of CO₂-assimilating Bacteria in Maize Field of Fluvo-aquic Soil in North China

LIU Hongmei(), HAI Xiang, AN Kerui, ZHANG Haifang, WANH Hui, ZHANG Yanjun, WANG Lili, ZHANG Guilong, YANG Dianlin

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, P. R. China

Received:2021-11-22 Online:2022-04-18 Published:2022-06-22

不同施肥措施对华北潮土区玉米田土壤固碳细菌群落结构多样性的影响

刘红梅(), 海香, 安克锐, 张海芳, 王慧, 张艳军, 王丽丽, 张贵龙, 杨殿林

农业农村部环境保护科研监测所,天津 300191

作者简介:刘红梅（1976年生）,女,副研究员,主要从事生物多样性与生态农业研究。E-mail: liuhongmei@caas.cn
基金资助:
国家重点研发计划项目(2018YFD0800905-3)

Abstract

Abstract:

To reveal the characteristics and influencing factors of different long-term fertilization regimes on the community structure of CO₂-assimilating bacteria in the maize field in North China in order to provide a theoretical basis and data support for sustainable fertilization management in North China. This study was conducted in the Wuqing Experimental Station of the Agro-Environmental Protection Institute of the Ministry of Agriculture and Rural Affairs. The field experiment included six treatments, no fertilizer (A0), organic fertilizer (A1), reduced nitrogen fertilizer plus organic fertilizer (A2), normal chemical fertilizer plus organic fertilizer treatment (A3), increased nitrogen fertilizer plus organic fertilizer (A4), and chemical fertilizer (A5). Soil samples were collected independently from the 0?20 cm soil layer. High-throughput sequencing technology was applied to test the changes in the soil community structure diversity of CO₂-assimilating bacteria under different fertilization treatments. The results showed that (1) compared with the A0 treatment, A1 and A3 increased the Shannon index, and A4 and A5 decreased the Shannon index. (2) In terms of the community composition, the dominant bacterial phyla was Proteobacteria (92.83%?94.98%); Compared with the A0 treatment, A4 and A5 significantly reduced the relative abundance of Proteobacteria, while A1, A2 and A3 showed no significant difference. The dominant bacterial class was Gammaproteobacteria (53.75%?65.80%); Compared with the A0 treatment, A2, A3, A4 and A5 significantly reduced the relative abundance of Gammaproteobacteria, but A1 showed no significant difference. (3) The results of the principal component analysis showed that the OTUs of the five fertilization treatments were significantly different from that of the control, and A3, A4 and A5 treatments were in different sorting regions from the controls, A0, A1, and A2. (4) The results of redundancy analysis indicated that soil pH, total nitrogen, total phosphorus, nitrate nitrogen, ammonium nitrogen, microbial biomass carbon, and microbial biomass nitrogen were significantly correlated with the soil cbbL-harboring bacterial community structure. In conclusion, long-term incremental application of the nitrogen fertilizer plus organic fertilizer and chemical fertilizer could reduce the diversity of the soil carbon sequestration bacterial community; Different fertilization measures affected the community structure of soil carbon sequestration bacteria by changing soil chemical properties.

Key words: fertilization regimes, fluvo-aquic soil, CO₂-assimilating bacteria, cbbL gene, community structure, high-throughput sequencing

摘要：

研究不同施肥措施下华北潮土区玉米田土壤固碳细菌群落特征及其影响因子,为华北地区可持续施肥管理提供理论依据和数据支持。以农业农村部环境保护科研监测所建立的武清长期定位试验站为平台,利用高通量测序技术,研究6种不同施肥处理[不施肥对照 (A0)、单施有机肥 (A1)、氮肥减量配施有机肥 (A2)、常量化肥配施有机肥 (A3)、氮肥增量配施有机肥 (A4) 和单施化肥 (A5)]对华北潮土区土壤固碳细菌结构及多样性的影响。结果表明,（1）与对照A0相比,A1和A3处理提高了Shannon多样性指数,A4和A5处降低了Shannon多样性指数。（2）群落组成方面,在门水平上,变形菌门（Proteobacteria）（92.83%—4.98%）为优势菌门;与对照A0相比,A4和A5处理显著降低了变形菌门相对丰度,A1、A2和A3无显著差异。在纲水平上,优势菌纲为γ-变形菌纲Gammaproteobacteria（53.75%—65.80%）;与对照A0相比,A2、A3、A4和A5处理显著降低了γ-变形菌纲相对丰度,A1处理与对照A0无显著差异。（3）主成分分析表明,5个施肥处理OTUs与对照处理差别显著,且A3、A4和A5处理与对照A0、A1和A2处于不同排序区。（4）冗余分析结果表明,土壤pH、全氮、全磷、硝态氮、铵态氮、微生物量碳和微生物量氮与土壤cbbL细菌群落结构变化显著相关。以上结果表明,长期氮肥增量配施有机肥和单施化肥可能降低土壤固碳细菌群落多样性;不同施肥措施通过改变土壤化学性质,影响了土壤固碳细菌群落结构。

关键词: 施肥措施, 潮土, 固碳细菌, cbbL基因, 群落结构, 高通量测序

CLC Number:

S154.1
X176

LIU Hongmei, HAI Xiang, AN Kerui, ZHANG Haifang, WANH Hui, ZHANG Yanjun, WANG Lili, ZHANG Guilong, YANG Dianlin. Effects of Different Fertilization Regimes on Community Structure Diversity of CO₂-assimilating Bacteria in Maize Field of Fluvo-aquic Soil in North China[J]. Ecology and Environment, 2022, 31(4): 715-722.

刘红梅, 海香, 安克锐, 张海芳, 王慧, 张艳军, 王丽丽, 张贵龙, 杨殿林. 不同施肥措施对华北潮土区玉米田土壤固碳细菌群落结构多样性的影响[J]. 生态环境学报, 2022, 31(4): 715-722.

Figures/Tables 6

References 27

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施肥处理 Fertilizationtreatments	pH	有机碳质量分数 w_{(total organic carbon)}/ (g∙kg^-1)	全氮质量分数 w_{(total nitrogen)}/ (g∙kg^-1)	全磷质量分数 w_{(total phosphorus)}/ (g∙kg^-1)	硝态氮质量分数 w_{(nitrate-nitrogen)}/ (mg∙kg^-1)	铵态氮质量分数 w_{(ammonium-nitrogen)}/ (mg∙kg^-1)	微生物量碳质量分数 w_{(microbial biomass carbon)}/ (mg∙kg^-1)	微生物量氮质量分数 w_{(microbial biomass nitrogen)}/ (mg∙kg^-1)
A0	8.71±0.03a	8.97±0.11d	1.23±0.18e	0.98±0.01d	6.39±0.10e	1.17±0.08c	365.97±32.76d	69.12±6.04b
A1	8.47±0.09bc	11.03±0.19b	2.08±0.04bc	1.54±0.03c	8.34±0.28d	1.22±0.08c	433.49±57.76cd	86.62±11.14a
A2	8.52±0.04b	9.94±0.30c	1.96±0.01c	1.55±0.04c	10.29±0.23c	1.70±0.22a	530.46±25.20ab	97.88±12.30a
A3	8.29±0.10de	11.37±0.25b	2.21±0.07b	1.64±0.03b	11.00±0.07bc	1.62±0.22ab	466.44±30.10bc	94.79±0.37a
A4	8.19±0.07e	12.28±0.29a	2.43±0.09a	1.89±0.01a	12.77±0.32a	1.37±0.13bc	557.25±53.33a	89.06±6.33a
A5	8.39±0.03cd	8.08±0.14e	1.64±0.21d	1.02±0.01d	11.39±0.97b	1.70±0.13a	389.52±10.71d	68.89±4.94b

施肥处理 Fertilizationtreatments	pH	有机碳质量分数 w_{(total organic carbon)}/ (g∙kg^-1)	全氮质量分数 w_{(total nitrogen)}/ (g∙kg^-1)	全磷质量分数 w_{(total phosphorus)}/ (g∙kg^-1)	硝态氮质量分数 w_{(nitrate-nitrogen)}/ (mg∙kg^-1)	铵态氮质量分数 w_{(ammonium-nitrogen)}/ (mg∙kg^-1)	微生物量碳质量分数 w_{(microbial biomass carbon)}/ (mg∙kg^-1)	微生物量氮质量分数 w_{(microbial biomass nitrogen)}/ (mg∙kg^-1)
A0	8.71±0.03a	8.97±0.11d	1.23±0.18e	0.98±0.01d	6.39±0.10e	1.17±0.08c	365.97±32.76d	69.12±6.04b
A1	8.47±0.09bc	11.03±0.19b	2.08±0.04bc	1.54±0.03c	8.34±0.28d	1.22±0.08c	433.49±57.76cd	86.62±11.14a
A2	8.52±0.04b	9.94±0.30c	1.96±0.01c	1.55±0.04c	10.29±0.23c	1.70±0.22a	530.46±25.20ab	97.88±12.30a
A3	8.29±0.10de	11.37±0.25b	2.21±0.07b	1.64±0.03b	11.00±0.07bc	1.62±0.22ab	466.44±30.10bc	94.79±0.37a
A4	8.19±0.07e	12.28±0.29a	2.43±0.09a	1.89±0.01a	12.77±0.32a	1.37±0.13bc	557.25±53.33a	89.06±6.33a
A5	8.39±0.03cd	8.08±0.14e	1.64±0.21d	1.02±0.01d	11.39±0.97b	1.70±0.13a	389.52±10.71d	68.89±4.94b

处理 Fertilization treatments	pH	有机碳 Total organic carbon	全氮 Total nitrogen	全磷 Total phosphorus	硝态氮 Nitrate nitrogen	铵态氮 Ammonium nitrogen	微生物量碳 Microbial biomass carbon	微生物量氮 Microbial biomass nitrogen
Chao1指数 Chao1 index	0.074	0.169	0.074	0.175	0.031	0.124	0.058	0.122
Observed_species指数 Observed_species index	0.025	0.188	0.049	0.148	0.035	0.119	-0.059	-0.014
PD_whole_tree指数 PD_whole_tree index	-0.038	0.084	-0.160	-0.060	-0.019	-0.170	-0.106	-0.291
Shannon指数 Shannon index	0.447^*	0.140	-0.030	0.147	-0.521^**	-0.187	-0.008	0.313

处理 Fertilization treatments	pH	有机碳 Total organic carbon	全氮 Total nitrogen	全磷 Total phosphorus	硝态氮 Nitrate nitrogen	铵态氮 Ammonium nitrogen	微生物量碳 Microbial biomass carbon	微生物量氮 Microbial biomass nitrogen
Chao1指数 Chao1 index	0.074	0.169	0.074	0.175	0.031	0.124	0.058	0.122
Observed_species指数 Observed_species index	0.025	0.188	0.049	0.148	0.035	0.119	-0.059	-0.014
PD_whole_tree指数 PD_whole_tree index	-0.038	0.084	-0.160	-0.060	-0.019	-0.170	-0.106	-0.291
Shannon指数 Shannon index	0.447^*	0.140	-0.030	0.147	-0.521^**	-0.187	-0.008	0.313

Effects of Different Fertilization Regimes on Community Structure Diversity of CO₂-assimilating Bacteria in Maize Field of Fluvo-aquic Soil in North China

不同施肥措施对华北潮土区玉米田土壤固碳细菌群落结构多样性的影响

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