Effects of Different Cropping Systems on Bacterial Community Structure and Diversity in Continuous Cropping Soil

doi:10.16258/j.cnki.1674-5906.2022.03.007

Ecology and Environment ›› 2022, Vol. 31 ›› Issue (3): 487-496.DOI: 10.16258/j.cnki.1674-5906.2022.03.007

• Research Articles • Previous Articles Next Articles

Effects of Different Cropping Systems on Bacterial Community Structure and Diversity in Continuous Cropping Soil

SONG Xiuli¹(), HUANG Ruilong¹, KE Caijie¹, HUANG Wei¹, ZHANG Wu¹^,^*(), TAO Bo²^,^*()

1. Lingnan Normal University, Zhanjiang 524048, P, R. China
2. Northeast Agricultural University, Harbin 150030, P, R. China

Received:2021-10-11 Online:2022-03-18 Published:2022-05-25
Contact: ZHANG Wu,TAO Bo

不同种植方式对连作土壤细菌群落结构和多样性的影响

宋秀丽¹(), 黄瑞龙¹, 柯彩杰¹, 黄蔚¹, 章武¹^,^*(), 陶波²^,^*()

1.岭南师范学院,广东湛江 524048
2.东北农业大学,黑龙江哈尔滨 150030

通讯作者: 章武,陶波
作者简介:宋秀丽（1984年生）,女,讲师,博士,主要研究方向土壤资源与土壤生态。E-mail: songxiuli5251@163.com
基金资助:
广东省基础与应用基础研究基金区域联合基金项目(2019A1515110888);国家重点研发计划(2018YFD0300103);岭南师范学院科学研究项目(ZL2044)

Abstract

Abstract:

Soybean continuous cropping harms soil microbial community. Rotation is conducive to the formation of soil microbial community diversity, but the impacts of different rotation systems on soil microbial community structure and diversity are not clear. This study used high-throughput sequencing technology to explore the effects of fallow (CK), fallow soybean rotation (FS), corn soybean rotation (CS), wheat soybean rotation (WS), and soybean continuous cropping (SC) on soil bacterial community diversity. The results of the principal component analysis showed that the soil bacterial community structure of the first four cropping systems was significantly different from that of soybean continuous cropping. The bacterial community structures of fallow and fallow soybean rotation were similar, and the same relationship was observed in corn soybean and wheat soybean rotation. The five cropping systems exhibited significant differences (P<0.05) in the values of soil bacterial Chao1 index, ACE index, and Shannon index. Fallow soybean treatment showed the highest values of bacterial Chao1 index, ACE index, and Shannon index, while continuous soybean treatment had the lowest values. The Venn diagram showed the largest number of unique OTUs under fallow treatment, followed by fallow soybean rotation, while corn soybean rotation had the smallest number. Beneficial soil bacteria, including Nitrospira, Anaerolinea, Azotobacter, and Methanosarcina, increased significantly with fallow soybean rotation. Compared to soybean continuous cropping, the relative abundance of the bacteria mentioned above increased by 0.47%, 0.29%, 0.37% and 0.12%, respectively. The relative abundance of Bacillus increased significantly in wheat soybean rotation. The chemical properties of soil under different cropping systems were significantly different. Furthermore, the chemical properties of organic matter (OM), available phosphorus (P), available copper (Cu), and available manganese (Mn) were significantly correlated with the composition of the soil bacterial community. In conclusion, different cropping systems affect the composition and diversity of soil bacterial community. Moreover, the differences in soil chemical properties under different cropping systems affect the composition of soil bacterial community structure. These results can provide a theoretical reference for the regulation of soil microecology.

Key words: cropping system, microbial diversity, bacterial community structure, high-throughput sequencing, soil chemical properties

摘要：

大豆连作对土壤微生物群落产生负面影响,轮作有利于土壤微生物群落多样性的形成,但关于不同轮作制度对连作土壤微生物群落结构和多样性的影响尚不明确。以东北黑土大豆连作土壤为研究对象,利用高通量测序技术,研究休耕（CK）、休耕-大豆（FS）、玉米-大豆（CS）、小麦-大豆（WS）和大豆连作（SC）5种种植方式对土壤细菌群落多样性的影响。结果表明,主成分分析显示前4种种植方式土壤细菌群落结构显著不同于SC,CK和FS土壤细菌群落结构相似,CS和WS土壤细菌群落结构相似。5种种植方式在土壤细菌Chao1指数、ACE指数和Shannon指数上呈现显著差异（P<0.05）,其中FS处理细菌Chao1指数、ACE指数和Shannon指数显著最高,SC处理最低。由OTUs韦恩图分析可知,CK处理特有的OTUs数量最多,FS次之,CS最少。与SC相比,有益菌属硝化螺旋菌（Nitrospira）、厌氧蝇菌（Anaerolinea）、固氮菌（Azotobacter）及甲烷八叠球菌（Methanosarcina）相对丰度在FS土壤中显著增高,分别增加了0.47%、0.29%、0.37%和0.12%,芽孢杆菌（Bacills）相对丰度在WS中显著增高。不同种植方式的土壤化学性质呈现显著差异,并且土壤中有机质（OM）和有效磷（P）、铜（Cu）、锰（Mn）等化学性质与土壤细菌群落组成显著相关。综上,不同种植方式影响土壤细菌群落组成和多样性,不同种植方式下土壤化学性质的差异影响土壤细菌群落结构组成。该研究结果可为破除连作障碍,引导种植方式提供理论参考。

关键词: 种植方式, 微生物多样性, 细菌群落结构, 高通量测序, 土壤化学性质

CLC Number:

SONG Xiuli, HUANG Ruilong, KE Caijie, HUANG Wei, ZHANG Wu, TAO Bo. Effects of Different Cropping Systems on Bacterial Community Structure and Diversity in Continuous Cropping Soil[J]. Ecology and Environment, 2022, 31(3): 487-496.

宋秀丽, 黄瑞龙, 柯彩杰, 黄蔚, 章武, 陶波. 不同种植方式对连作土壤细菌群落结构和多样性的影响[J]. 生态环境学报, 2022, 31(3): 487-496.

Figures/Tables 10

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土壤化学性质 Soil chemical properties	处理 Treatment
土壤化学性质 Soil chemical properties	CK	FS	CS	WS	SC
w_(OM)/(g∙kg^-1)	74.22±2.71a	60.53±8.41ab	54.12±1.44bc	46.04±1.91c	74.02±3.62a
w_(N)/(mg∙kg^-1)	318±11.86b	364±2.08ab	311±7.54b	248±8.39c	387±37.54a
w_(P)/(mg∙kg^-1)	29.33±0.88c	12.33±0.88e	38.33±1.20b	47.33±0.88a	25.00±0.58d
w_(K)/(mg∙kg^-1)	313±13.22a	228±0.58b	152±11.32c	231±2.52b	323±1.76a
pH	6.29±0.04a	6.13±0.05b	6.25±0.01a	6.19±0.04ab	6.24±0.01ab
w_(Cu)/(mg∙kg^-1)	0.84±0.04c	0.65±0.02d	1.33±0.04b	1.84±0.04a	1.29±0.01b
w_(Zn)/(mg∙kg^-1)	0.19±0.01c	0.46±0.09b	0.14±0.04c	0.50±0.03b	0.69±0.02a
w_(Fe)/(mg∙kg^-1)	136.86±0.55d	244±4.02b	208±9.10c	196±3.47c	304±0.69a
w_(Mn)/(mg∙kg^-1)	35.09±0.21a	10.91±0.23e	13.95±0.72d	16.48±0.54c	32.54±0.19b
w_(B)/(mg∙kg^-1)	0.37±0.03ab	0.30±0.00a	1.02±0.64a	0.96±0.11a	0.70±0.27a

土壤化学性质 Soil chemical properties	处理 Treatment
土壤化学性质 Soil chemical properties	CK	FS	CS	WS	SC
w_(OM)/(g∙kg^-1)	74.22±2.71a	60.53±8.41ab	54.12±1.44bc	46.04±1.91c	74.02±3.62a
w_(N)/(mg∙kg^-1)	318±11.86b	364±2.08ab	311±7.54b	248±8.39c	387±37.54a
w_(P)/(mg∙kg^-1)	29.33±0.88c	12.33±0.88e	38.33±1.20b	47.33±0.88a	25.00±0.58d
w_(K)/(mg∙kg^-1)	313±13.22a	228±0.58b	152±11.32c	231±2.52b	323±1.76a
pH	6.29±0.04a	6.13±0.05b	6.25±0.01a	6.19±0.04ab	6.24±0.01ab
w_(Cu)/(mg∙kg^-1)	0.84±0.04c	0.65±0.02d	1.33±0.04b	1.84±0.04a	1.29±0.01b
w_(Zn)/(mg∙kg^-1)	0.19±0.01c	0.46±0.09b	0.14±0.04c	0.50±0.03b	0.69±0.02a
w_(Fe)/(mg∙kg^-1)	136.86±0.55d	244±4.02b	208±9.10c	196±3.47c	304±0.69a
w_(Mn)/(mg∙kg^-1)	35.09±0.21a	10.91±0.23e	13.95±0.72d	16.48±0.54c	32.54±0.19b
w_(B)/(mg∙kg^-1)	0.37±0.03ab	0.30±0.00a	1.02±0.64a	0.96±0.11a	0.70±0.27a

多样性指数 Diversity index	CK	FS	CS	WS	SC
Chao1	4798±35b	5541±205a	4740±134b	4592±49b	4504±127b
ACE	4803±27b	5553±156a	4659±179b	4563±35b	4509±106b
Shannon	6.47±0.02b	6.76±0.08a	6.54±0.08b	6.57±0.03b	6.45±0.06b

多样性指数 Diversity index	CK	FS	CS	WS	SC
Chao1	4798±35b	5541±205a	4740±134b	4592±49b	4504±127b
ACE	4803±27b	5553±156a	4659±179b	4563±35b	4509±106b
Shannon	6.47±0.02b	6.76±0.08a	6.54±0.08b	6.57±0.03b	6.45±0.06b

处理 Treatment	潜在有益菌的相对丰度 Relative abundance of potentially beneficial bacteria
处理 Treatment	硝化螺旋菌 Nitrospira/%	根瘤菌 Rhizobium/%	芽孢杆菌 Bacillus/%	厌氧蝇菌 Anaerolinea/%×10	固氮菌 Azotobacter/%×10²	甲烷八叠球菌 Methanosarcina/%×10²
CK	0.32±0.01b	0.05±0.00b	0.01±0.00d	0.00±0.06b	0.00±0.00b	0.00±0.00b
FS	0.68±0.06a	0.05±0.01b	0.02±0.00d	0.31±0.01a	0.37±0.19a	0.12±0.12a
CS	0.34±0.01b	0.22±0.02a	0.07±0.00b	0.01±0.01b	0.00±0.00b	0.00±0.00b
WS	0.37±0.02b	0.22±0.04a	0.12±0.01a	0.00±0.00b	0.00±0.00b	0.00±0.00b
SC	0.21±0.02c	0.32±0.06a	0.05±0.01c	0.02±0.01b	0.00±0.00b	0.00±0.00b

Effects of Different Cropping Systems on Bacterial Community Structure and Diversity in Continuous Cropping Soil

不同种植方式对连作土壤细菌群落结构和多样性的影响

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References 48

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菌门 Phylum	有机质 OM	有效N Available N	有效P Available P	有效K Available K	pH	有效Cu Available Cu	有效Mn Available Mn
Proteobacteria		-0.59*	0.67**			0.82**
Acidobacteria	0.64*	0.55*	-0.72**			-0.81**
Bacteroidetes			0.60*		0.52*	0.60*	0.64*
Actinobacteria	-0.66**			-0.61*			-0.70**

菌属 Genus	菌门 Phylum	N	P	K	OM	pH	Cu	Zn	Fe	Mn
Gp4	Acidobacteria				0.52*		-0.66**	-0.68**	-0.55*
Gp6	Acidobacteria	0.65**	-0.67**		0.77**		-0.76**			0.56*
Gemmatimonas	Gemmatimonadetes		-0.71**				-0.70**
Gp1	Acidobacteria					-0.68**			0.71**	-0.61*
Gaiella	Actinobacteria						-0.60*			-0.54*
WPS-1	candidate_division_WPS-1		-0.63*			-0.53*	-0.55*			-0.70**
Flavobacterium	Bacteroidetes			0.80**	0.58*					0.91**
Gp3	Acidobacteria								0.68**
Rhizomicrobium	Proteobacteria				-0.71**					-0.71**
Rhodanobacter	Proteobacteria	-0.58*	0.55*	-0.58*	-0.74**		0.60*			-0.61
Terrimonas	Bacteroidetes				0.76**		-0.52*			0.66**
Opitutus	Verrucomicrobia		0.52				0.76**	0.54*
Pedobacter	Bacteroidetes	-0.71**	0.69**		-0.64**		0.77**

菌属 Genus	N	K	OM	pH	Zn	Fe	Mn
Nitrospira	0.78**		0.53*		0.52*	0.76**
Rhizobium	-0.56*	-0.71**	-0.75**				-0.79**
Bacillu		-0.59*		-0.52*			-0.84**
Anaerolinea	0.60*	0.53*			0.68**	0.73**
Azotobacter	0.57*				0.57*	0.61*
Methanosarcina	0.64*				0.52*	0.57*

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