Comparative Study on Soil Bacterial Diversity of Degraded Alpine Meadow in the Sanjiangyuan Region

doi:10.16258/j.cnki.1674-5906.2022.04.007

Ecology and Environment ›› 2022, Vol. 31 ›› Issue (4): 695-703.DOI: 10.16258/j.cnki.1674-5906.2022.04.007

• Research Articles • Previous Articles Next Articles

Comparative Study on Soil Bacterial Diversity of Degraded Alpine Meadow in the Sanjiangyuan Region

WANG Yingcheng¹(), YAO Shiting¹, JIN Xin¹, YU Wenzhen², LU Guangxin¹^,^*(), WANG Junbang³^,^*()

1. Qinghai University, Xining 810016, P. R. China
2. School of Geographical Sciences, Nanjing 210044, P. R. China
3. National Ecosystem Science Data Center, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, P. R. China

Received:2021-11-04 Online:2022-04-18 Published:2022-06-22
Contact: LU Guangxin,WANG Junbang

三江源区高寒退化草甸土壤细菌多样性的对比研究

王英成¹(), 姚世庭¹, 金鑫¹, 俞文政², 芦光新¹^,^*(), 王军邦³^,^*()

1.青海大学,青海西宁 810016
2.南京信息工程大学,江苏南京
3.中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室/生态系统大数据与模拟中心,北京 100101

通讯作者: 芦光新,王军邦
作者简介:王英成（1995年生）,女,博士研究生,研究方向高寒草地微生物多样性及功能利用。E-mail: 1343014868@qq.com
基金资助:
国家自然科学基金项目(U20A2098);国家自然科学基金项目(31860103);青海省“昆仑英才·高端创新创业人才”项目

Abstract

Abstract:

In order to explore the structure and diversity of soil bacterial communities in the undegraded meadow and degraded meadow in the Sanjiangyuan Region, we systematically analyzed the soil bacterial diversity, community composition and structure, and their interactions with environmental factors, using the nested sampling method and high throughput sequencing technology. The results showed that the alpine meadow degradation had an impact on both vegetation and soil. Alpine meadow degradation reduced the vegetation coverage and richness, increased the soil pH value, and resulted in a transition to alkaline soil, while the contents of total nitrogen, organic matter, and electrical conductivity in soil decreased significantly (P<0.05). The high-throughput sequencing identified 2168457 effective bacterial sequences and 76798 OTUs. The α-diversity and species composition of soil bacterial community were significantly affected by grassland degradation (P<0.05). The Observed Richness, Shannon Index, and Chao 1 Index were significantly higher in the undegraded alpine meadow than those in the degraded alpine meadow. Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Verrucomicrobia, and Thaumarchaeota were the dominant phylum. Alpine meadow degradation increased the relative abundance of Actinobacteria and Bacteroidetes, but decreased the relative abundance of Verrucomicrobia. The PCoA and Dissimilarity tests showed that Alpine meadow degradation significantly changed the soil bacterial community structure. Soil heterogeneity had a certain effect on soil bacterial community. The main environmental factors driving the soil bacterial community were soil pH, soil organic matter content, and soil total nitrogen content. Our research systematically analyzed the variation of soil bacterial diversity in degraded alpine meadows, revealed the diversity and composition of soil bacterial communities, and provided a reference for the future research related to the regulation of soil microorganisms on meadow degradation.

Key words: alpine meadow, grassland degradation, high-throughput sequencing, soil bacterial, microbial diversity, community structure

摘要：

为探究三江源区未退化高寒草甸与退化高寒草甸土壤细菌群落结构及多样性,采用巢氏取样方法和高通量测序技术,系统地对土壤细菌群落多样性、群落组成和结构及其与环境因子间的关系进行研究分析。结果表明,草地退化对植被和土壤都产生影响,草地退化降低了植被盖度和丰富度,使得土壤pH值增加,土壤向碱性化过渡,而土壤全氮、有机质和电导率的含量显著降低（P<0.05）。高通量测序得到优质有效细菌序列2168457条和71798个OTUs。草地退化对土壤细菌群落α多样性和物种组成都有显著影响（P<0.05）。其中,草甸退化导致土壤细菌物种指数（Observed Richness）、多样性指数（Shannon Index）和chao 1指数上升;变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）、疣微菌门（Verrucomicrobia）、奇古菌门（Thaumarchaeota）为主要优势菌门;草甸退化提高了放线菌门（Actinobacteria）、拟杆菌门（Bacteroidetes）的相对丰度,但却降低了疣微菌门（Verrucomicrobia）的相对丰度。通过PCoA和Dissimilarity检验的方法表明草地退化显著改变了退化草地土壤细菌群落结构。土壤异质性对土壤细菌群落具有一定的影响,其中,驱动土壤细菌群落的主要环境因子是土壤pH、有机质含量和总氮含量。该研究分析了细菌群落多样性和组成结构,揭示了退化高寒草甸土壤细菌群落多样性变化规律,为土壤微生物对草地退化方面的研究提供了参考。

关键词: 高寒草甸, 退化草地, 高通量测序, 土壤细菌, 微生物多样性, 群落结构

CLC Number:

S154
X171.1

WANG Yingcheng, YAO Shiting, JIN Xin, YU Wenzhen, LU Guangxin, WANG Junbang. Comparative Study on Soil Bacterial Diversity of Degraded Alpine Meadow in the Sanjiangyuan Region[J]. Ecology and Environment, 2022, 31(4): 695-703.

王英成, 姚世庭, 金鑫, 俞文政, 芦光新, 王军邦. 三江源区高寒退化草甸土壤细菌多样性的对比研究[J]. 生态环境学报, 2022, 31(4): 695-703.

Figures/Tables 10

References 44

[1]	ALLISON S D, LU Y, WEIHE C, GOULDEN M L, et al., 2013. Microbial abundance and composition influence litter decomposition response to environmental change[J]. Ecology: A Publication of the Ecological Society of America, 94(3): 714-725.
[2]	BARDGETT R D, LEEMANS D K, COOK R, et al., 1997. Seasonality of soil biota of the grazed and ungrazed hill grasslands[J]. Soil Biology and Biochemistry, 29(8): 1285-1294. DOI URL
[3]	BIER R L, BERNHARDT E S, BOOT C M, et al., 2015. Linking microbial community structure and microbial processes: An empirical and conceptual overview[J]. Fems Microbiology Ecology, 91(10): 1-11.
[4]	CHE R X, DENG Y C, WANG F, et al., 2015. 16S rRNA-based bacterial community structure is a sensitive indicator of soil respiration activity[J]. Journal of Soils and Sediments, 15(9): 1987-1990. DOI URL
[5]	CHE R X, WANG Y F, LI K X, et al., 2019. Degraded patch formation significantly changed microbial community composition in alpine meadow soils[J]. Soil and Tillage Research, 195: 104426. DOI URL
[6]	EDGAR R C, 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 10(10): 996-998. DOI URL
[7]	FAN D D, KONG W D, WANG F, et al., 2020. Fencing decreases microbial diversity but increases abundance in grassland soils on the Tibetan Plateau[J]. Land Degradation and Development, 31(17): 2577-2590. DOI URL
[8]	FENG K, ZHANG Z J, CAI W W, et al., 2017. Biodiversity and species competition regulate the resilience of microbial biofilm community[J]. Molecular Ecology, 26(21): 6170-6182. DOI URL
[9]	GU S S, HU Q L, CHENG Y Q, et al., 2019. Application of organic fertilizer improves microbial community diversity and alters microbial network structure in tea (Camellia sinensis) plantation soils[J]. Soil and Tillage Research, 195: 104356. DOI URL
[10]	GUO G X, KONG W D, LIU J B, et al., 2015. Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau[J]. Applied Microbiology and Biotechnology, 99: 8765-8776. DOI URL
[11]	GUO J X, LI J S, LIU K S, et al., 2018. Analysis of soil microbial dynamics at a cropland-grassland interface in an agro-pastoral zone in a temperate steppe in northern China[J]. Catena, 170: 257-265. DOI URL
[12]	HAN D X, WANG N, SUN X, et al., 2018. Biogeographical distribution of bacterial communities in Changbai Mountain, Northeast China[J]. Microbiology Open, 7: e00529. DOI URL
[13]	HANEY C H, SAMUEL B S, BUSH J, et al., 2015. Associations with rhizosphere bacteria can confer an adaptive advantage to plants[J]. Nature Plants, 1(6): 15051. DOI URL
[14]	HOU X L, HAN H, TIGABU M, et al., 2019. Changes in soil physico-chemical properties following vegetation restoration ediate bacterial community composition and diversity in Changting, China[J]. Ecology Engineering, 138: 171-179.
[15]	KONG Y, 2011. Btrim: A fast, lightweight adapter and quality trimming program for next-generation sequencing technologies[J]. Genomics, 98(2): 152-153. DOI URL
[16]	LI Y M, WANG S P, JIANG L L, et al., 2016. Changes of soil microbial community under different degraded gradients of alpine meadow[J]. Agriculture Ecosystems Environment, 222: 213-222. DOI URL
[17]	LI Y, LI J J, ARE K S, et al., 2019. Livestock grazing significantly accelerates soil erosion more than climate change in Qinghai-Tibet Plateau: evidenced from 137Cs and 210Pbex measurements[J]. Agriculture, Ecosystems and Environment, 285: 106643. DOI URL
[18]	MAGOC T, SALZBERG S L, 2011. FLASH: fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics, 27(21): 2957-2963. DOI URL
[19]	MARTINY J B H, EISEN J A, PENN K, et al., 2011. Drivers of bacterial β-diversity depend on spatial scale[J]. Proceedings of the National Academy of Sciences of the United States of America, 108(19): 7850-7854.
[20]	Olff H, HOORENS B, GOEDE R G M D, et al., 2000. Small-scale shifting mosaics of two dominant grassland species: the possible role of soil-borne pathogens[J]. Oecologia, 125(1): 45-54. DOI PMID
[21]	TORSVIK V, ØVREÅS L, THINGSTAD T F, 2001. Prokaryotic diversity: Magnitude, dynamics, and controlling factors[J]. Science, 296(5570): 1064-1066. DOI URL
[22]	WANG C T, WANG G X, WANG Y, et al., 2016. Fire alters vegetation and soil microbial community in alpine meadow[J]. Land Degraded and Development, 27(5): 1379-1390.
[23]	WANG X J, ZHANG Z C, YU Z Q, et al., 2020. Composition and diversity of soil microbial communities in the alpine wetland and alpine forest ecosystems on the Tibetan Plateau[J]. Science of the Total Environment, 747: 141358. DOI URL
[24]	WANG Y S, LI C N, KOU Y P, et al., 2017. Soil pH is a major driver of soil diazotrophic community assembly in Qinghai-Tibet alpine meadows[J]. Soil Biology and Biochemistry, 115: 547-555. DOI URL
[25]	WU G L, REN G H, DONG Q M, et al., 2014. Above- and belowground response along degradation gradient in an Alpine Grassland of the Qinghai-Tibetan Plateau[J]. Clean Soil Air Water, 42(3): 319-323. DOI URL
[26]	ZHANG X, ZHANG R, GAO J, et al., 2017. Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria[J]. Soil Biology and Biochemistry, 104: 208-217. DOI URL
[27]	ZHOU H, ZHANG D, JIANG Z, et al., 2019. Changes in the soil microbial communities of alpine steppe at Qinghai-Tibetan Plateau under different degradation levels[J]. Science of The Total Environment, 651(Part 2): 2281-2291. DOI URL
[28]	陈乐乐, 施建军, 王彦龙, 等, 2016. 高寒地区不同退化程度草地群落结构特征研究[J]. 草地学报, 24(1): 210-213.
	CHEN L L, SHI J J, WANG Y L, et al., 2016. Study on different degraded degrees grassland community structure characteristics of the Alpine area[J]. Acta Agrestia Sinica, 24(1): 210-213.
[29]	党宁, 2020. 三江源区高寒草地土壤微生物群落多样性与环境因子关系的研究[D]. 西宁: 青海大学.
	DANG N, 2020. Study on the Relationship between Soil Microbial Community Diversity and Environmental Factors in Alpine Grassland in the Three-Rivers Headwaters Region[D]. Xining: Qinghai University.
[30]	贺纪正, 李晶, 郑袁明, 2013. 土壤生态系统微生物多样性—稳定性关系的思考[J]. 生物多样性, 21(4): 411-420. DOI
	HE J Z, LI J, ZHENG Y M, 2013. Thoughs on the microbial diversity-stability relationship in soil ecosystems[J]. Biodiversity Science, 21(4): 411-420. DOI URL
[31]	李海云, 姚拓, 马亚春, 等, 2019. 祁连山中段退化高寒草地土壤细菌群落分布特征[J]. 草业学报, 28(8): 170-179.
	LI H Y, YAO T, MA Y C, et al., 2019. Soil bacterial community changes across a degradation gradient in alpine meadow grasslands in the central Qilian Mountains[J]. Acta Prataculturae Sinica, 28(8): 170-179.
[32]	李雪萍, 李建宏, 刘永刚, 等, 2020. 甘南草原不同退化草地植被和土壤微生物特性[J]. 草地学报, 28(5): 1252-1259.
	LI X P, LI J H, LIU Y G, et al., 2020. The vegetation and soil microorganism characteristics of different degraded grassland in gannan steppe[J]. Acta Agrestia Sinica, 28(5): 1252-1259.
[33]	刘洋荧, 王尚, 厉舒祯, 等, 2017. 基于功能基因的微生物碳循环分子生态学研究进展[J]. 微生物学通报, 44(7): 1676-1689.
	LIU Y Y, WANG S, LI S Z, et al., 2017. Advances in molecular ecology on microbial functional genes of carbon cycle[J]. Microbiology China, 44(7): 1676-1689.
[34]	刘玉, 马玉寿, 施建军, 等, 2013. 大通河上游高寒草甸植物群落的退化特征[J]. 草业科学, 30(7): 1082-1088.
	LIU Y, MA Y S, SHI J J, et al., 2013. Community characteristics of alpine meadow under different degree of degradation in the upper area of Daitong River[J]. Pratacultural Science, 30(7): 1082-1088.
[35]	王启兰, 曹广民, 王长庭, 2007. 高寒草甸不同植被土壤微生物数量及微生物生物量的特征[J]. 生态学杂志, 26(7): 1002-1008.
	WANG Q L, CAO G M, WANG C T, 2007. Quantitative characters of soil microbes and microbial biomass under different vegetations in alpine meadow[J]. Chinese Journal of Ecology, 26(7): 1002-1008.
[36]	王英成, 芦光新, 赵丽蓉, 等, 2021. 高寒草甸退化对土壤电导率变化影响的研究[J]. 干旱区研究, 38(1): 104-113.
	WANG Y C, LU G X, ZHAO L R, et al., 2021. The influence of alpine meadow degradation on soil conductivity change[J]. Arid Zone Research, 38(1): 104-113.
[37]	王朱珺, 王尚, 刘洋荧, 等, 2018. 宏基因组技术在氮循环功能微生物分子检测研究中的应用[J]. 生物技术通报, 34(1): 1-6. DOI
	WANG Z J, WANG S, LIU Y Y, et al., 2018. The Applications of Metagenomics in the Detection of Environmental Microbes Involving in Nitrogen Cycle[J]. Biotechnology Bulletin, 34(1): 1-6.
[38]	薛凯, 张彪, 周姝彤, 等, 2019. 青藏高原高寒草地土壤微生物群落及影响因子[J]. 种学通报, 64(27): 2915-2927.
	XUE K, ZHANG B, ZHOU S T, et al., 2019. Soil microbial communities in alpine grasslands on the Tibetan Plateau and their influencing factors[J]. Chinese Science Bulletin, 64(27): 2915-2927.
[39]	杨建平, 丁永建, 沈永平, 等, 2004. 近40年来江河源区生态环境变化的气候特征分析[J]. 冰川冻土, 26(1): 7-16.
	YANG J P, DING Y J, SHEN Y P, et al., 2004. Climatic features of eco-environment change in the source regions of the Yangtze and Yellow Rivers in recent 40 years[J]. Journal of Glaciology and Geocryology, 26(1): 7-16.
[40]	杨希智, 王长庭, 字洪标, 等, 2015. 三江源区不同建植年限人工草地土壤微生物群落结构特征[J]. 应用与环境生物学报, 21(2): 341-349.
	YANG X Z, WANG C T, ZI H B, et al., 2015. Soil microbial community structure characteristics in arti oil microbial community structure characteristics in artificial grassland with cial grassland with different cultivation years in the headwater region of Three Rivers, China[J]. Chinese Journal of Applied and Environmental Biology, 21(2): 341-349.
[41]	姚拓, 龙瑞军, 2006. 天祝高寒草地不同扰动生境土壤三大类微生物数量动态研究[J]. 草业学报, 15(2): 93-99.
	YAO T, LONG R J, 2006. Dynamics of soil microbial population under disturbance in Tianzhu Alpine grassland[J]. Acta Prataculturae Sinica, 15(2): 93-99.
[42]	姚世庭, 芦光新, 邓晔, 等, 2021. 模拟增温对土壤真菌群落组成及多样性的影响[J]. 生态环境学报, 30(7): 1404-1411.
	YAO S T, LU G X, DENG Y, et al., 2021. Effects of simulated warming on soil fungal community composition and diversity[J]. Ecology and Environmental Sciences, 30(7): 1404-1411
[43]	张卫信, 申智峰, 邵元虎, 等, 2020. 土壤生物与可持续农业研究进展[J]. 生态学报, 40(10): 3183-3206.
	ZHANG W X, SHEN Z F, SHAO Y H, et al., 2020. Soil biota and sustainable agriculture: A review[J]. Acta Ecologica Sinica, 40(10): 3183-3206.
[44]	赵轻舟, 王艳芬, 崔骁勇, 等, 2018. 草地土壤微生物多样性影响因素研究进展[J]. 生态科学, 37(3): 204-212.
	ZHAO Q Z, WANG Y F, CUI X Y, et al., 2018. Research progress of the influence factors of soil microbial diversity in grassland[J]. Ecological Science, 37(3): 204-212.

植被名称 Vegetation	未退化草地 Undegraded alpine meadow	退化草地 Degraded alpine meadow
矮生忍冬 Lanicera minuta	10.40±3.16	16.34±6.76
车前草 Plantago major	—	5.59±0.00
臭蒿 Artemisia hedinii	—	6.04±0.00
独活 Heracleum millefolium	—	10.09±4.47
独一味 Lamiophlomis rotata	8.26±2.46	—
多裂委陵菜 Potentilla multifida	4.91±3.08	7.77±2.18
甘青大戟 Euphorbia micractina	4.48±0.91	9.08±3.06
高山嵩草 Kobresia pygmaea	47.58±10.16	41.97±17.73
高山唐松草 Thalictrum alpinum	—	12.16±11.25
海乳草 Glaux maritima	—	5.54±0.00
黄芪 Astragalus tanguticus	7.28±0.00	11.23±3.80
火绒草 Leontopodium leontopodioides	6.43±2.95	7.65±4.86
黄花棘豆 Oxytropis ochrocephala	8.52±4.65	9.05±5.17
赖草 Leymus secalinus	8.82±0.00	7.67±0.39
兰石草 Lancea tibetica	—	9.45±5.38
麻花艽 Gentiana straminea	6.87±1.60	10.23±3.07
美丽风毛菊 Saussurea superba	8.88±6.37	7.51±0.00
蒲公英 Taraxacum mongolicum	4.30±1.48	3.18±0.96
青藏龙胆 Gentiana futtereri	6.74±2.49	8.79±2.11
狮牙风毛菊 Saussurea leontodontoides	7.33±0.00	—
苔草 Carex spp.	—	11.35±0.00
兔耳草 Lagotis brevituba	7.13±5.32	7.76±0.58
细叶亚菊 Ajania tenuifolia	9.22±2.62	16.69±6.71
星状风毛菊 Saussurea stella	8.16±2.07	—
雪灵芝 Arenaria kansuensis Maxim.	4.26±1.86	11.72±8.91
重冠紫菀 Aster diplostephioides	14.17±7.38	16.19±12.82
紫花针茅 Stipa purpurea	21.35±4.83	19.47±4.39
植被盖度 Vegetation cover/%	87	72
物种数 Species	7.26±1.24a	6.18±1.24b
地上生物量 Aboveground biomass/(g∙m^-2)	69.01±25.75a	59.86±26.52b

植被名称 Vegetation	未退化草地 Undegraded alpine meadow	退化草地 Degraded alpine meadow
矮生忍冬 Lanicera minuta	10.40±3.16	16.34±6.76
车前草 Plantago major	—	5.59±0.00
臭蒿 Artemisia hedinii	—	6.04±0.00
独活 Heracleum millefolium	—	10.09±4.47
独一味 Lamiophlomis rotata	8.26±2.46	—
多裂委陵菜 Potentilla multifida	4.91±3.08	7.77±2.18
甘青大戟 Euphorbia micractina	4.48±0.91	9.08±3.06
高山嵩草 Kobresia pygmaea	47.58±10.16	41.97±17.73
高山唐松草 Thalictrum alpinum	—	12.16±11.25
海乳草 Glaux maritima	—	5.54±0.00
黄芪 Astragalus tanguticus	7.28±0.00	11.23±3.80
火绒草 Leontopodium leontopodioides	6.43±2.95	7.65±4.86
黄花棘豆 Oxytropis ochrocephala	8.52±4.65	9.05±5.17
赖草 Leymus secalinus	8.82±0.00	7.67±0.39
兰石草 Lancea tibetica	—	9.45±5.38
麻花艽 Gentiana straminea	6.87±1.60	10.23±3.07
美丽风毛菊 Saussurea superba	8.88±6.37	7.51±0.00
蒲公英 Taraxacum mongolicum	4.30±1.48	3.18±0.96
青藏龙胆 Gentiana futtereri	6.74±2.49	8.79±2.11
狮牙风毛菊 Saussurea leontodontoides	7.33±0.00	—
苔草 Carex spp.	—	11.35±0.00
兔耳草 Lagotis brevituba	7.13±5.32	7.76±0.58
细叶亚菊 Ajania tenuifolia	9.22±2.62	16.69±6.71
星状风毛菊 Saussurea stella	8.16±2.07	—
雪灵芝 Arenaria kansuensis Maxim.	4.26±1.86	11.72±8.91
重冠紫菀 Aster diplostephioides	14.17±7.38	16.19±12.82
紫花针茅 Stipa purpurea	21.35±4.83	19.47±4.39
植被盖度 Vegetation cover/%	87	72
物种数 Species	7.26±1.24a	6.18±1.24b
地上生物量 Aboveground biomass/(g∙m^-2)	69.01±25.75a	59.86±26.52b

环境因子 Environmental Factors	变量 Variables	未退化草甸 Undegraded	退化草甸 Degraded
土壤参数 Edaphic parameters	w_(TN)/%	69.06±8.99a	58.01±11.91b
	w_(NH4+-N)/(mg∙kg^-1)	45.02±13.77a	45.94±7.19a
	w_(NO3--N)/(mg∙kg^-1)	136.21±27.95a	132.91±38.50a
	w_(OM)/%	10.60±2.39a	6.85±2.02b
	土壤含水量 w_(SM)/%	26.43±7.42a	24.79±4.73a
	土壤电导率 EC/(μS∙cm^-1)	0.23±0.19a	0.13±0.11b
	pH	6.33±0.13b	7.44±0.28a

环境因子 Environmental Factors	变量 Variables	未退化草甸 Undegraded	退化草甸 Degraded
土壤参数 Edaphic parameters	w_(TN)/%	69.06±8.99a	58.01±11.91b
	w_(NH4+-N)/(mg∙kg^-1)	45.02±13.77a	45.94±7.19a
	w_(NO3--N)/(mg∙kg^-1)	136.21±27.95a	132.91±38.50a
	w_(OM)/%	10.60±2.39a	6.85±2.02b
	土壤含水量 w_(SM)/%	26.43±7.42a	24.79±4.73a
	土壤电导率 EC/(μS∙cm^-1)	0.23±0.19a	0.13±0.11b
	pH	6.33±0.13b	7.44±0.28a

皮尔森相关性 Pearson	未退化草甸 Undegraded alpine meadow			退化草甸 Degraded alpine meadow
皮尔森相关性 Pearson	香农指数 Shannon Index	辛普森指数 Simpson Index	丰富度指数 Observed Richness	香农指数 Shannon Index	辛普森指数 Simpson Index	丰富度指数 Observed Richness
总氮含量 TN	0.018	0.159	-0.097	-0.120	-0.095	-0.196
铵态氮含量 NH₄⁺-N	-0.113	-0.247*	-0.066	0.056	0.096	0.055
硝态氮含量 NO₃^--N	0.060	-0.007	0.059	-0.003	0.056	0.021
有机质含量 OM	-0.137	-0.037	-0.141	0.091	-0.065	0.086
土壤含水量 SM	0.030	-0.130	0.082	0.145	0.048	0.203
土壤电导率 EC	0.219	0.174	0.195	-0.197	-0.071	-0.243*
pH	-0.197	-0.202	-0.137	0.085	0.035	0.148

Comparative Study on Soil Bacterial Diversity of Degraded Alpine Meadow in the Sanjiangyuan Region

三江源区高寒退化草甸土壤细菌多样性的对比研究

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 44

Related Articles 15

Recommended Articles

Metrics

Comments

不相似性检验 Dissimilarity test		细菌群落 Bacterial community
不相似性检验 Dissimilarity test		Bray Curtis	Jaccard
未退化草甸vs退化草甸 Undegraded vs Degraded	MRPP	0.460***	0.653***
	ANOSIM	0.365***	0.426***
	PERMANOVA	19.684***	7.466***

环境因子 Environmental factors	BC		JC
环境因子 Environmental factors	r	P	r	P
总氮含量 TN	0.112**	0.006	0.114**	0.002
铵态氮含量 NH₄⁺-N	-0.068	0.951	-0.057	0.943
硝态氮含量 NO₃^--N	0.120**	0.010	0.075*	0.021
有机质含量 OM	0.137**	0.005	0.131***	0.001
pH	0.319***	0.001	0.362***	0.001
土壤含水量 SM	-0.073	0.988	-0.068	0.988
土壤电导率 EC	-0.028	0.699	-0.012	0.604

[1]	HOU Hui, YAN Peixuan, XIE Qinmi, ZHAO Hongliang, PANG Danbo, CHEN Lin, LI Xuebin, HU Yang, LIANG Yongliang, NI Xilu. Characterization of Arbuscular Mycorrhizal Fungal Community Diversity in the Rhizosphere Soils of Prunus mongolica Scrub of Helan Mountain [J]. Ecology and Environment, 2023, 32(5): 857-865.
[2]	CHEN Junfang, WU Xian, LIU Xiaolin, LIU Juan, YANG Jiarong, LIU Yu. Shaping Characteristics of Elemental Stoichiometry on Microbial Diversity under Different Soil Water Contents [J]. Ecology and Environment, 2023, 32(5): 898-909.
[3]	JIANG Yongwei, DING Zhenjun, YUAN Junbin, ZHANG Zheng, LI Yang, WEN Qingchun, WANG Yeyao, JIN Xiaowei. Study on Benthic Macroinvertebrates Community Structure and Water Quality Evaluation in Main Rivers of Liaoning Province [J]. Ecology and Environment, 2023, 32(5): 969-979.
[4]	WANG Yun, ZHENG Xilai, CAO Min, LI Lei, SONG Xiaoran, LIN Xiaolei, GUO Kai. Study on Denitrification Performance and Control Factors in Brackish-Freshwater Transition Zone of Coastal Aquifer [J]. Ecology and Environment, 2023, 32(5): 980-988.
[5]	KOU Zhu, QING Chun, YUAN Changguo, LI Ping. Diversity and Distribution of Sulfur Oxidizing Bacteria in Hot Springs of Northeast Tibet, China [J]. Ecology and Environment, 2023, 32(5): 989-1000.
[6]	YANG Chunliang, LIU Minxia, WANG Qianyue, MIAO Lele, XIAO Yindi, WANG Min. Spatial Pattern and Correlation of Populations of Anemone rivularis and Kobresia myosuroides under Single-household Management and Multi-household Management Grazing Patterns [J]. Ecology and Environment, 2023, 32(4): 651-659.
[7]	HU Fang, LIU Jutao, WEN Chunyun, HAN Liu, WEN Hui. Phytoplankton Community Structure and Evaluation of Aquatic Ecological Conditions in Fu River Basin [J]. Ecology and Environment, 2023, 32(4): 744-755.
[8]	YU Fei, ZENG Hailong, FANG Huaiyang, FU Lingfang, LIN Shu, DONG Jiahao. Spatio-temporal Variation Characteristics of Phytoplankton Functional Groups and Water Quality Evaluation in the Typical Tidal River Network [J]. Ecology and Environment, 2023, 32(4): 756-765.
[9]	QIN Hao, LI Mengai, GAO Jin, CHEN Kailong, ZHANG Yinbo, ZHANG Feng. Composition and Diversity of Soil Bacterial Communities in Shrub at Different Altitudes in Luya Mountain [J]. Ecology and Environment, 2023, 32(3): 459-468.
[10]	WANG Jie, SHAN Yan, MA Lan, SONG Yanjing, WANG Xiangyu. Effects of Straw and Biochar Synergistic Returning on the Improvement of Salt-affected Soil in the Yellow River Delta [J]. Ecology and Environment, 2023, 32(1): 90-98.
[11]	WANG Lixiao, LIU Jinxian, CHAI Baofeng. Response of Soil Bacterial Community and Nitrogen Cycle during the Natural Recovery of Abandoned Farmland in Subalpine of the North China [J]. Ecology and Environment, 2022, 31(8): 1537-1546.
[12]	ZHOU Xuanbo, WANG Xiaoli, MA Yushou, WANG Yanlong, LUO Shaohui, XIE Lele. Niche of Main Plant Populations in Alpine Meadow Under the Rest-grazing in the Green-Up Period [J]. Ecology and Environment, 2022, 31(8): 1547-1555.
[13]	HUA Li, CHENG Taozhi, LIANG Zhiyong. Remediation Effect of Petroleum-Contaminated Soil by Immobilized Mixed Bacteria in Northern Shaanxi Province of China [J]. Ecology and Environment, 2022, 31(8): 1610-1615.
[14]	GU Chen, JIA Zhiqing, DU Bobo, HE Lingxianzi, LI Qingxue. Reviews and Prospects of Ecological Restoration Measures for Degraded Grasslands of China [J]. Ecology and Environment, 2022, 31(7): 1465-1475.
[15]	ZHU Yihao, LI Qingmei, LIU Xiaoli, LI Na, SONG Fengling, CHEN Weifeng. Characteristics of Soil Microbial Community in Newly Cultivated Land under Different Land Consolidation Types [J]. Ecology and Environment, 2022, 31(5): 909-917.