Soil microbial community structure and diversity of potato rhizosphere and non-rhizosphere were studied by using Illumina-MiSeq high-throughput sequencing technologies (soil becteria, 16S rDNA gene; soil fungi, 18 s rDNA gene), and then explore the relationships between soil microbial community structure and diversity and soil nutrients, providing theoretical data for the healthily planting potato. The results showed that: (1) The pH of potato in rhizosphere soil was significantly lower than that of non-rhizosphere (P<0.05), and the conductivity, organic carbon, available nitrogen and available phosphorus of rhizosphere soil were significantly higher than that of non-rhizosphere (P<0.05), while the difference between total phosphorus and non-rhizosphere soil was not significant (P>0.05). (2) The richness index of bacteria and fungi, shannon-wiener index, ACE index and Chao 1 index in potato rhizosphere soil were significantly higher than those in non-rhizosphere soil (P<0.05). However, there were no significant difference in the coverage, uniformity and Simpson index between the rhizosphere and the non-rhizosphere (P>0.05). (3) The dominant soil bacterial phylum in rhizosphere and non-rhizosphere soil were Proteobacteria, Acidobacteria, Gemmatimonadetes, followed by Planctomycetaceae, Actinobacteria, Bacteroidetes, Firmicutes, Chloroflexi, Verrucomicrobia. The relative abundance of Acidobacteria in rhizosphere was higher than non-rhizosphere soil, while the relative abundance of Proteobacteria in rhizosphere was lower than non-rhizosphere soil. Similarly, the dominant soil fungal phylum in rhizosphere and non-rhizosphere soil were Ascomycota and Basidiomycota, followed by Zygomycota, Chytridiomycota, Neocallimastigomycota, Glomeromycota, Blastocladiomycota. (4) Principal component analysis (PCA) analysis showed that bacterial and fungal communities of potato in rhizosphere and non-rhizosphere soil had good similarity, and bacterial communities in rhizosphere and non-rhizosphere had obvious separation effect. Pearson correlation analysis showed that there was no significant correlation between soil bacteria and fungus Coverage and ACE and soil nutrients (P>0.05). Soil pH was negatively correlated with soil bacterial and fungal diversity, while soil electrical conductivity and total phosphorus were not significantly correlated with soil bacterial and fungal diversity (P>0.05). And (5) redundancy analysis showed that 7 soil environmental factors explained 86% of the total characteristic values of bacteria and 82% of fungi, respectively, indicating that soil environmental factors had a significant impact on the diversity of bacteria and fungi in potato soil. Among them, SOC and TN had great influence on soil bacterial and fungal diversity, while pH had negative influence on soil bacterial and fungal diversity.