Characterization of Arbuscular Mycorrhizal Fungal Community Diversity in the Rhizosphere Soils of Prunus mongolica Scrub of Helan Mountain

doi:10.16258/j.cnki.1674-5906.2023.05.004

Ecology and Environment ›› 2023, Vol. 32 ›› Issue (5): 857-865.DOI: 10.16258/j.cnki.1674-5906.2023.05.004

• Research Articles • Previous Articles Next Articles

Characterization of Arbuscular Mycorrhizal Fungal Community Diversity in the Rhizosphere Soils of Prunus mongolica Scrub of Helan Mountain

HOU Hui¹^,²(), YAN Peixuan³, XIE Qinmi¹^,², ZHAO Hongliang³, PANG Danbo¹^,²^,⁴, CHEN Lin¹^,²^,⁴, LI Xuebin¹^,², HU Yang¹^,²^,⁴, LIANG Yongliang⁵, NI Xilu¹^,²^,⁴^,^*()

1. Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China/School of Ecological and Environment, Ningxia University, Yinchuan 750021, P. R. China
2. Key Lab. of Restoration and Reconstruction of Degraded Ecosystems in northwestern China of Ministry of Education/Ningxia University, Yinchuan 750021, P. R. China
3. College of Agriculture, Ningxia University, Yinchuan 750021, P. R. China
4. Ningxia Helan Mountain Forest Ecosystem Orientation Observation Research Station, Yinchuan 750021, P. R. China
5. Ningxia Helan Mountain National Nature Reserve Management Bureau, Yinchuan 750021, P. R. China

Received:2022-12-10 Online:2023-05-18 Published:2023-08-09
Contact: NI Xilu

贺兰山蒙古扁桃灌丛根际土壤AM真菌群落多样性特征研究

侯晖¹^,²(), 颜培轩³, 谢沁宓¹^,², 赵宏亮³, 庞丹波¹^,²^,⁴, 陈林¹^,²^,⁴, 李学斌¹^,², 胡杨¹^,²^,⁴, 梁咏亮⁵, 倪细炉¹^,²^,⁴^,^*()

1.宁夏大学生态环境学院/西北土地退化与生态恢复国家重点实验室培育基地，宁夏银川 750021
2.宁夏大学/西北退化生态系统恢复与重建教育部重点实验室，宁夏银川 750021
3.宁夏大学农学院，宁夏银川 750021
4.宁夏贺兰山森林生态系统定位观测研究站，宁夏银川 750021
5.宁夏贺兰山国家级自然保护区管理局，宁夏银川 750021

通讯作者: 倪细炉
作者简介:侯晖（1998年生），男，硕士研究生，研究方向为山地生态，植物生态等。E-mail: 2447508658@qq.com
基金资助:
宁夏重点研发项目(2020BFG03006);宁夏重点研发项目(2021BFG02005);宁夏自然科学基金项目(2020AAC03107);国家自然科学基金项目(41761066);国家自然科学基金项目(42067022)

Abstract

Abstract:

Drought-tolerant shrubs and herbs were important vegetation types in the low-to-medium altitude of the Helan Mountain, and it is important to reveal the diversity of rhizosphere soil arbuscular mycorrhizal fungal (AMF) communities and their influencing factors for stability of ecosystem function, growth and development of vegetation and healthy development in the arid and semi-arid areas of Helan Mountain. We selected a typical Prunus mongolica-Stipa breviflora communities (G×S-S，G×S-G) in the low altitude of Helan Mountain, and we set up two controlled treatments, in which S. breviflora (G) had shrubs removed and P. mongolica (S) had herbs removed, and investigated differences in the structure and diversity of rhizosphere soil AMF communities and interrelationships with environmental factors in the P. mongolica-S. breviflora communities and single vegetation by high-throughput sequencing methods. The results showed that soil pH, organic matter, total N, available P and available K content were the largest under G×S-G and G×S-S; soil alkali-hydrolyzable N and total P content were second to G; α diversity index of rhizosphere soil AMF community were the largest and all the differences were significant except for total N (P<0.05), which was consistent with the "fertile island" effect of the shrubland. A total of 174 operational taxonomic units (OTUs) were obtained from rhizosphere soil AMF communities. These OTUs could be classified into 1 phylum, 4 class, 6 orders, 10 families, 10 genera and 63 species, and the dominant genus of AMF was Glomus, accounting for 99.26% on average, The Ambispora among four treatments (P<0.05) was significantly different. Non-metric multidimensional scaling analysis (NMDS) indicated that rhizosphere soil AMF community structure showed differences at the OTU level. Correlation and redundancy analysis (RDA) indicated that rhizosphere soil available K, available P and N were significantly correlated with AMF community α-diversity and species abundance (P<0.05, P<0.01). This study aims to provide theoretical references for the conservation of ecosystem functions and vegetation restoration in arid and semi-arid regions.

Key words: arbuscular mycorrhizal fungi, community structure, Prunus mongolica, Stipa breviflora, Helan Mountain

摘要：

耐旱灌木和草本植物是贺兰山中低海拔的重要植被类型，揭示其根际土壤丛枝菌根真菌（arbuscular mycorrhizal fungi，AMF）群落多样性及其影响因子，对于贺兰山干旱半干旱地区生态系统功能的稳定，植被的生长发育及健康发展具有重要意义。以贺兰山低海拔区蒙古扁桃-短花针茅群落（G×S-S，G×S-G）为研究对象，设置群落内去除灌木蒙古扁桃（Prunus mongolica）的短花针茅（Stipa breviflora）（G）和去除草本植物的蒙古扁桃（S）两种对照处理，利用高通量测序方法研究其根际土壤AMF群落结构与多样性差异及其与环境因子间的相互关系。结果表明：G×S-G和G×S-S的根际土壤pH、有机质、全氮、速效磷和速效钾含量最大，碱解氮和全磷含量次于G，根际土壤AMF群落α多样性指数最大，除全氮外呈显著性差异（P<0.05），符合灌丛“沃岛”效应。根际土壤AMF共获得174个操作分类单元（operational taxonomic unit，OTU），隶属于1门4纲6目10科10属63种，优势属为球囊霉属（Glomus），平均占比为99.26%，两性球囊霉属（Ambispora）在4种处理间呈显著性差异（P<0.05）。非度量多维尺度分析（non-metric multidimensional scaling，NMDS）分析表示，根际土壤AMF群落结构在OTU水平上呈差异性。相关性及冗余分析（redundancy analysis，RDA）表明，根际土壤速效钾、磷、氮等与AMF群落α多样性和物种丰度显著相关（P<0.05，P<0.01）。该研究旨为干旱半干旱地区生态系统功能的养护、植被的修复提供理论参考。

关键词: 丛枝菌根真菌, 群落结构, 蒙古扁桃, 短花针茅, 贺兰山

CLC Number:

S154.36
X172

HOU Hui, YAN Peixuan, XIE Qinmi, ZHAO Hongliang, PANG Danbo, CHEN Lin, LI Xuebin, HU Yang, LIANG Yongliang, NI Xilu. Characterization of Arbuscular Mycorrhizal Fungal Community Diversity in the Rhizosphere Soils of Prunus mongolica Scrub of Helan Mountain[J]. Ecology and Environment, 2023, 32(5): 857-865.

侯晖, 颜培轩, 谢沁宓, 赵宏亮, 庞丹波, 陈林, 李学斌, 胡杨, 梁咏亮, 倪细炉. 贺兰山蒙古扁桃灌丛根际土壤AM真菌群落多样性特征研究[J]. 生态环境学报, 2023, 32(5): 857-865.

Figures/Tables 9

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样地	经度	纬度	植被类型	海拔/m
G1	105.944649°E	38.749245°N	草丛	1703.48
G2	105.943912°E	38.749996°N	草丛	1720.72
G3	105.941699°E	38.753279°N	草丛	1748.35
G4	105.943152°E	38.754502°N	草丛	1764.84
G5	105.943190°E	38.754588°N	草丛	1763.29
S1	105.942007°E	38.753104°N	灌木	1754.69
S2	105.941848°E	38.753473°N	灌木	1743.33
S3	105.941730°E	38.753524°N	灌木	1742.79
S4	105.941796°E	38.753541°N	灌木	1744.21
S5 G×S 1 G×S 2 G×S 3 G×S 4 G×S 5	105.941943°E 105.945033°E 105.944993°E 105.944751°E 105.944692°E 105.943945°E	38.753060°N 38.749928°N 38.749652°N 38.749302°N 38.749134°N 38.749854°N	灌木灌草灌草灌草灌草灌草	1742.15 1722.47 1717.13 1714.55 1702.56 1720.32

样地	经度	纬度	植被类型	海拔/m
G1	105.944649°E	38.749245°N	草丛	1703.48
G2	105.943912°E	38.749996°N	草丛	1720.72
G3	105.941699°E	38.753279°N	草丛	1748.35
G4	105.943152°E	38.754502°N	草丛	1764.84
G5	105.943190°E	38.754588°N	草丛	1763.29
S1	105.942007°E	38.753104°N	灌木	1754.69
S2	105.941848°E	38.753473°N	灌木	1743.33
S3	105.941730°E	38.753524°N	灌木	1742.79
S4	105.941796°E	38.753541°N	灌木	1744.21
S5 G×S 1 G×S 2 G×S 3 G×S 4 G×S 5	105.941943°E 105.945033°E 105.944993°E 105.944751°E 105.944692°E 105.943945°E	38.753060°N 38.749928°N 38.749652°N 38.749302°N 38.749134°N 38.749854°N	灌木灌草灌草灌草灌草灌草	1742.15 1722.47 1717.13 1714.55 1702.56 1720.32

样地类型	pH	w_(有机质)/(g·kg^-1)	w_(全氮)/(mg·kg^-1)	w_(碱解氮)/(mg·kg^-1)	w_(全磷)/(mg·kg^-1)	w_(速效磷)/(mg·kg^-1)	w_(速效钾)/(mg·kg^-1)
G	8.22±0.04b¹⁾	20.51±1.24ab	30.80±9.00a	56.91±1.79a	242.54±8.42a	7.39±1.73a	168.19±5.34b
S	8.21±0.01b	20.04±0.42b	29.87±3.37a	27.72±2.55c	123.89±1.48b	5.98±0.46b	94.80±14.43c
G×S-G G×S-S	8.30±0.01a 8.22±0.02b	22.79±0.43a 21.71±0.64ab	41.07±9.74a 31.73±5.20a	37.80±0.63b 37.80±0.96b	167.11±2.25ab 184.94±3.97ab	8.97±0.22a 7.56±0.26a	234.90±16.18a 192.21±19.38ab

样地类型	pH	w_(有机质)/(g·kg^-1)	w_(全氮)/(mg·kg^-1)	w_(碱解氮)/(mg·kg^-1)	w_(全磷)/(mg·kg^-1)	w_(速效磷)/(mg·kg^-1)	w_(速效钾)/(mg·kg^-1)
G	8.22±0.04b¹⁾	20.51±1.24ab	30.80±9.00a	56.91±1.79a	242.54±8.42a	7.39±1.73a	168.19±5.34b
S	8.21±0.01b	20.04±0.42b	29.87±3.37a	27.72±2.55c	123.89±1.48b	5.98±0.46b	94.80±14.43c
G×S-G G×S-S	8.30±0.01a 8.22±0.02b	22.79±0.43a 21.71±0.64ab	41.07±9.74a 31.73±5.20a	37.80±0.63b 37.80±0.96b	167.11±2.25ab 184.94±3.97ab	8.97±0.22a 7.56±0.26a	234.90±16.18a 192.21±19.38ab

变量	pH	有机质	全氮	碱解氮	全磷	速效磷	速效钾
Shannon指数	0.482*	0.338	-0.695**	0.493*	-0.234	0.446*	0.545**
Simpson指数	-0.469*	-0.266	0.586**	-0.477*	0.166	-0.308	-0.523**
ACE指数	0.267	0.496*	-0.329	0.105	-0.210	0.437*	0.463*
Chao1指数	0.254	0.492*	-0.352	0.003	-0.255	0.389*	0.482*
球囊霉属 Glomus	-0.238	-0.246	0.244	-0.170	0.445*	-0.447*	-0.545**
近明球囊霉属 Claroideoglomus	0.225	0.101	-0.278	0.161	-0.499*	0.337	0.445*
其他属 Others	0.041	0.434*	0.087	0.030	0.112	0.340	0.310

Characterization of Arbuscular Mycorrhizal Fungal Community Diversity in the Rhizosphere Soils of Prunus mongolica Scrub of Helan Mountain

贺兰山蒙古扁桃灌丛根际土壤AM真菌群落多样性特征研究

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