Effect of Calcium Modification on the Binding of Biochar-derived Dissolved Organic Matter with Cd(II)

doi:10.16258/j.cnki.1674-5906.2025.07.012

Ecology and Environmental Sciences ›› 2025, Vol. 34 ›› Issue (7): 1121-1132.DOI: 10.16258/j.cnki.1674-5906.2025.07.012

• Research Article [Environmental Science] • Previous Articles Next Articles

Effect of Calcium Modification on the Binding of Biochar-derived Dissolved Organic Matter with Cd(II)

HE Huan¹^,²(), ZHOU Dandan¹^,²^,^*(), MA Zhixuan¹^,², LI Fangfang¹^,², QIN Shanshan¹^,², DOU Sixian¹^,²

1. Faculty of Environmental Science & Engineering, Kunming University of Science & Technology, Kunming 650500, P. R. China
2. Yunnan Key Laboratory of Soil Carbon Sequestration and Pollution Control, Kunming 650500, P. R. China

Received:2025-01-08 Online:2025-07-18 Published:2025-07-11

钙改性对生物炭中溶解性有机质与Cd(Ⅱ)结合的影响

贺环¹^,²(), 周丹丹¹^,²^,^*(), 马芷萱¹^,², 李芳芳¹^,², 秦珊珊¹^,², 豆思娴¹^,²

1.昆明理工大学环境科学与工程学院，云南昆明 650500
2.云南省土壤固碳与污染控制重点实验室，云南昆明 650500

通讯作者: *E-mail: 01yongheng@163.com
作者简介:贺环（1994年生），女，硕士研究生，主要研究方向为生物炭环境效应及污染物环境行为研究。E-mail: 1639477032@qq.com
基金资助:
国家自然科学基金青年科学基金项目(41703121);云南省基础研究计划项目(202301AT070472);昆明理工大学人才启动项目(KKSY201722006)

Abstract

Abstract:

Calcium-modified biochar (CaBC) has a large specific surface area, a rich variety of functional groups, and a high content of mineral elements, which promote the effective remediation of cadmium pollution. However, the release of dissolved organic matter (BDOM) with significant fluidity during biochar application affects the environmental behavior of Cd(II). Currently, the release characteristics of calcium-modified BDOM and its binding to Cd(II) remain largely unknown. In this study, we investigated the effects of calcium modification on the BDOM characteristics using parallel factor analysis and two-dimensional correlation spectroscopy. The results indicated that 1) calcium modification significantly increased BDOM release, especially for aromatic, hydrophobic, and larger molecular weight components; 2) calcium modification enhanced the relative abundance of humic-like substances and promoted their first release, but inhibited the release rate of protein-like components, leading to a reduction in their content and relative abundance. The experimental results on the complexation of BDOM with Cd(II) indicated that: 1) the fulvic-like fraction of calcium-modified BDOM binds to Cd(II) at the fastest rate, which is related to its highest relative distribution; 2) calcium modification enhances the complexation ability of protein-like substances with Cd(II), but the proportion of binding with Cd(II) decreases, which is associated with a lower content and relative distribution of protein-like substances after modification; and 3) the increase in molecular weight and aromaticity enhances the complexation capacity of BDOM with Cd(II) and the stability of the formed complexes. This study is helpful in understanding the effect of calcium modification on the binding characteristics of BDOM and its Cd(II), and provides a reference for the application of calcium-modified biochar in cadmium pollution remediation.

Key words: calcium modified biochar, dissolved organic matter, spectral analysis, cadmium, complexation

摘要：

钙改性生物炭（CaBC）具有比表面积大、官能团种类丰富、矿质元素含量高等特性，对镉污染修复效果好。然而，生物炭施用过程中释放具有显著流动性的溶解性有机质（BDOM）会影响Cd(Ⅱ) 的环境行为。目前，关于钙改性生物炭中BDOM的释放特性及与其Cd(Ⅱ) 的结合特征尚不清晰。采用平行因子和二维相关光谱分析研究钙改性对生物炭BDOM特性的影响，其结果表明：1）钙改性使生物炭BDOM释放量显著增加，尤其是芳香性、疏水性及较大分子量组分；2）钙改性增加了类腐殖酸的相对分布并使其最先释放，但其抑制了类蛋白质的释放速度且类蛋白质含量和相对分布减少。BDOM与Cd(Ⅱ) 的络合实验结果表明：1）钙改性生物炭BDOM中类富里酸与Cd(Ⅱ) 结合速度最快，这与类富里酸相对分布最高有关；2）钙改性提高类蛋白质与Cd(Ⅱ) 的络合能力，但与Cd(Ⅱ) 的结合比例降低，这与改性后类蛋白质含量和相对分布较少有关；3）分子量和芳香性的增加促使BDOM与Cd(Ⅱ) 络合能力及形成的络合物稳定性增加。该研究有助于了解钙改性对BDOM与Cd(Ⅱ) 的结合特征的影响，并为钙改性生物炭在镉污染修复方面的应用提供参考依据。

关键词: 钙改性生物炭, 溶解性有机质, 光谱分析, 镉, 络合

CLC Number:

HE Huan, ZHOU Dandan, MA Zhixuan, LI Fangfang, QIN Shanshan, DOU Sixian. Effect of Calcium Modification on the Binding of Biochar-derived Dissolved Organic Matter with Cd(II)[J]. Ecology and Environmental Sciences, 2025, 34(7): 1121-1132.

贺环, 周丹丹, 马芷萱, 李芳芳, 秦珊珊, 豆思娴. 钙改性对生物炭中溶解性有机质与Cd(Ⅱ)结合的影响[J]. 生态环境学报, 2025, 34(7): 1121-1132.

Figures/Tables 11

References 63

[1]	ALBRECHT R, LE PETIT J, TERROM G, et al., 2011. Comparison between UV spectroscopy and nirs to assess humification process during sewage sludge and green wastes co-composting[J]. Bioresource Technology, 102(6): 4495-4500. DOI PMID
[2]	BAI H C, JIANG Z M, HE M J, et al., 2018. Relating Cd²⁺ binding by humic acids to molecular weight: A modeling and spectroscopic study[J]. Journal of Environmental Sciences, 70: 154-165.
[3]	BIAN R J, JOSEPH S, SHI W, et al., 2019. Biochar DOM for plant promotion but not residual biochar for metal immobilization depended on pyrolysis temperature[J]. Science of The Total Environment, 662: 571-580.
[4]	BRO R, 1997. PARAFAC. Tutorial and applications[J]. Chemometrics and Intelligent Laboratory Systems, 38(2): 149-171.
[5]	CHEN K, YANG Y M, ZHAO H, et al., 2023. Study on the cadmium and copper binding characteristics of dissolved organic matter released from human-feces-biochar (HFDOM) using parallel factor analysis (PARAFAC) and two-dimensional correlation spectroscopy (2D-COS)[J]. Environmental Science and Pollution Research, 30: 46900-46912.
[6]	CUI M, XU D Y, LIU X B, et al., 2024. Influence of spectral and molecular composition of dissolved organic matter on labile Cd mobility in riparian soils in the Three Gorges Reservoir, China[J]. Science of The Total Environment, 955: 176736.
[7]	ELBISHLAWI H, JAFFE P R, 2015. Characterization of dissolved organic matter from a restored urban marsh and its role in the mobilization of trace metals[J]. Chemosphere, 127: 144-151. DOI PMID
[8]	FENG D D, SUN H L, MA Y, et al., 2020. Catalytic mechanism of K and Ca on the volatile-biochar interaction for rapid pyrolysis of biomass: Experimental and simulation studies[J]. Energy & Fuels, 34: 9741-9753.
[9]	GAO Z Y, SHAN D X, HE J H, et al., 2023. Effects and mechanism on cadmium adsorption removal by CaCl₂-modified biochar from selenium-rich straw[J]. Bioresource Technology, 370: 128563.
[10]	GUI X Y, LIU C, LI F Y, et al., 2020. Effect of pyrolysis temperature on the composition of DOM in manure-derived biochar[J]. Ecotoxicology and Environmental Safety, 197: 110597.
[11]	GUO X J, PENG Y Y, LI N X, et al., 2022. Effect of biochar-derived DOM on the interaction between Cu(II) and biochar prepared at different pyrolysis temperatures[J]. Journal of Hazardous Materials, 421: 126739.
[12]	HAMEED R, LI G L, SON Y H, et al., 2023. Structural characteristics of dissolved black carbon and its interactions with organic and inorganic contaminants: A critical review[J]. Science of The Total Environment, 872: 162210.
[13]	HE C J, HE X W, LI J J, et al., 2021. The spectral characteristics of biochar-derived dissolved organic matter at different pyrolysis temperatures[J]. Journal of Environmental Chemical Engineering, 9(5): 106075.
[14]	HE C J, HE X W, YUAN R, et al., 2022. Binding characteristics of Pb and Zn to low-temperature feces-based biochar-derived DOM revealed by EEM-PARAFAC combined with general and moving-window two-dimensional correlation analyses[J]. Environmental Science and Pollution Research, 30: 27525-27538.
[15]	HE W, HUR J, 2015. Conservative behavior of fluorescence EEM-PARAFAC components in resin fractionation processes and its applicability for characterizing dissolved organic matter[J]. Water Research, 83: 217-226. DOI PMID
[16]	HUANG M, LI Z W, CHEN M, et al., 2020. Dissolved organic matter released from rice straw and straw biochar: Contrasting molecular composition and lead binding behaviors[J]. Science of The Total Environment, 739: 140378.
[17]	HUANG M, LI Z W, LUO N L, et al., 2019. Application potential of biochar in environment: Insight from degradation of biochar-derived DOM and complexation of DOM with heavy metals[J]. Science of The Total Environment, 646: 220-228.
[18]	HUANG M, LIAO Z, LI Z W, et al., 2022. Effects of pyrolysis temperature on proton and cadmium binding properties onto biochar-derived dissolved organic matter: Roles of fluorophore and chromophore[J]. Chemosphere, 299: 134313.
[19]	JI Y N, ZHENG N, AN Q R, et al., 2024. Enhanced immobilization of cadmium and lead in contaminated soil using calcium alginate-modified HAP biochar: Improvements in soil health and microbial diversity[J]. Environmental Pollution, 357: 124445.
[20]	JIANG S J, DAI G L, RASHID M S, et al., 2024. Effects of BC on metal uptake by crops (availability) and the vertical migration behavior in soil: A 3-year field experiments of crop rotation[J]. Chemosphere, 350: 141075.
[21]	JIN C S, LI Z W, HURSTHOUSE A S, et al., 2023. Manganese oxides mediated dissolve organic matter compositional changes in lake sediment and cadmium binding characteristics[J]. Ecotoxicology and Environmental Safety, 256: 114916.
[22]	LENG E W, WANG Y, GONG X, et al., 2017. Effect of KCl and CaCl₂ loading on the formation of reaction intermediates during cellulose fast pyrolysis[J]. Proceedings of the Combustion Institute, 36(2): 2263-2270.
[23]	LI D Q, LI C, FAN M J, et al., 2023a. Investigation of property of biochar in staged pyrolysis of cellulose[J]. Journal of Analytical and Applied Pyrolysis, 172: 105999.
[24]	LI D M, WANG Z Y, YANG Y X, et al., 2023b. Characterization of copper binding to different molecular weight fractions of dissolved organic matter in surface water[J]. Journal of Environmental Management, 341: 118067.
[25]	LI G, KHAN S, IBRAHIM M, et al., 2018. Biochars induced modification of dissolved organic matter (DOM) in soil and its impact on mobility and bioaccumulation of arsenic and cadmium[J]. Journal of Hazardous Materials, 348: 100-108. DOI PMID
[26]	LI H B, DONG X L, DA SILVA E B, et al., 2017. Mechanisms of metal sorption by biochars: Biochar characteristics and modifications[J]. Chemosphere, 178: 466-478. DOI PMID
[27]	LI L P, LIU Y H, REN D, et al., 2022. Characteristics and chlorine reactivity of biochar-derived dissolved organic matter: Effects of feedstock type and pyrolysis temperature[J]. Water Research, 211: 118044.
[28]	LIANG T, ZHOU G P, CHANG D N, et al., 2024. The dissolved organic matter from the co-decomposition of Chinese milk vetch and rice straw induces the strengthening of Cd remediation by Fe-modified biochar[J]. Biochar, 6: 27.
[29]	LIU C H, CHU W Y, LI H, et al., 2019. Quantification and characterization of dissolved organic carbon from biochars[J]. Geoderma, 335: 161-169.
[30]	LIU J B, YANG W T, ZHOU H, et al., 2024a. Exploring the mechanisms of organic fertilizers on Cd bioavailability in rice fields: Environmental behavior and effect factors[J]. Ecotoxicology and Environmental Safety, 285: 117094.
[31]	LIU X R, WEI L H, JIANG J Y, et al., 2024b. New insights into the effect of pyrolysis temperature on the spectroscopy properties of dissolved organic matter in manure-based biochar[J]. Environmental Science and Pollution Research, 31: 18527-18539.
[32]	LIU Y C, GAO Z L, JI X G, et al., 2023. Efficient Adsorption of tebuconazole in aqueous solution by calcium modified water hyacinth-based biochar: Adsorption kinetics, mechanism, and feasibility[J]. Molecules, 28(8): 3478.
[33]	MURPHY K R, BUTLER K D, SPENCER R G M, et al., 2010. Measurement of dissolved organic matter fluorescence in aquatic environments: An interlaboratory comparison[J]. Environmental Science & Technology, 44(24): 9405-9412.
[34]	NAN H Y, YIN J X, YANG F, et al., 2021. Pyrolysis temperature-dependent carbon retention and stability of biochar with participation of calcium: Implications to carbon sequestration[J]. Environmental Pollution, 287: 117566.
[35]	NODA I, 2012. Close-up view on the inner workings of two-dimensional correlation spectroscopy[J]. Vibrational Spectroscopy, 60: 146-153.
[36]	OU Q, XU Y H, HE Q, et al., 2021. Deposition behavior of dissolved black carbon on representative surfaces: Role of molecular conformation[J]. Journal of Environmental Chemical Engineering, 9(5): 105921.
[37]	REN N N, TANG Y Y, LI M, 2018. Mineral additive enhanced carbon retention and stabilization in sewage sludge-derived biochar[J]. Process Safety and Environmental Protection, 115: 70-78.
[38]	STEDMON C A, BRO R, 2008. Characterizing dissolved organic matter fluorescence with parallel factor analysis: A tutorial[J]. Limnology and Oceanography: Methods, 6(11): 572-579.
[39]	SUN D Z, LI F Y, JIN J W, et al., 2022. Qualitative and quantitative investigation on adsorption mechanisms of Cd(II) on modified biochar derived from co-pyrolysis of straw and sodium phytate[J]. Science of The Total Environment, 829: 154599.
[40]	WANG C C, ZHANG Q C, YAN C A, et al., 2023a. Heavy metal(loid)s in agriculture soils, rice, and wheat across China: Status assessment and spatiotemporal analysis[J]. Science of The Total Environment, 882: 163361.
[41]	WANG J B, KANG Y X, DUAN H T, et al., 2022. Remediation of Cd²⁺ in aqueous systems by alkali-modified (Ca) biochar and quantitative analysis of its mechanism[J]. Arabian Journal of Chemistry, 15: 103750.
[42]	WANG S Y, WANG Y J, WANG X Y, et al., 2024. Study on adsorption of Cd in solution and soil by modified biochar-calcium alginate hydrogel[J]. Gels, 10(6): 388.
[43]	WANG X S, YU G, LIN H, et al., 2020. Advances in bioremediation of cadmium-contaminated soils[J]. IOP Conference Series: Earth and Environmental Science, 508: 012006.
[44]	WANG Y F, LI J E, XU L, et al., 2023b. The effect and spectral response mechanism of dissolved organic matter (DOM) in Pb(II) adsorption onto biochar[J]. Journal of Environmental Chemical Engineering, 11(5): 111115.
[45]	WU J, ZHANG H, SHAO L M, et al., 2012. Fluorescent characteristics and metal binding properties of individual molecular weight fractions in municipal solid waste leachate[J]. Environmental Pollution, 162: 63-71. DOI PMID
[46]	WU W Z, YAN B B, ZHONG L, et al., 2021. Combustion ash addition promotes the production of K-enriched biochar and K release characteristics[J]. Journal of Cleaner Production, 311: 127557.
[47]	XIAO R, WANG J J, GASTON L A, et al., 2018. Biochar produced from mineral salt-impregnated chicken manure: Fertility properties and potential for carbon sequestration[J]. Waste Management, 78: 802-810. DOI PMID
[48]	XIAO Y, WIESNER M R, 2013. Transport and retention of selected engineered nanoparticles by porous media in the presence of a biofilm[J]. Environmental Science & Technology, 47(5): 2246-2253.
[49]	XIU L Q, GU W Q, SUN Y Y, et al., 2023. The fate and supply capacity of potassium in biochar used in agriculture[J]. Science of The Total Environment, 902: 165969.
[50]	XU Y G, QI F J, YAN Y B, et al., 2023. The interaction of different chlorine-based additives with swine manure during pyrolysis: Effects on biochar properties and heavy metal volatilization[J]. Waste Management, 169: 52-61. DOI PMID
[51]	YU S H, ZHANG H Y, NI J Z, et al., 2023. Spectral characteristics coupled with self-organizing maps analysis on different molecular size-fractionated water-soluble organic carbon from biochar[J]. Science of The Total Environment, 857(Part 2): 159424.
[52]	ZEPP R G, SHELDON W M, MORAN M A, 2004. Dissolved organic fluorophores in southeastern US coastal waters: Correction method for eliminating Rayleigh and Raman scattering peaks in excitation-emission matrices[J]. Marine Chemistry, 89(1-4): 15-36.
[53]	ZHANG H Y, QIAN W, WU L, et al., 2022b. Spectral characteristics of dissolved organic carbon (DOC) derived from biomass pyrolysis: Biochar-derived DOC versus smoke-derived DOC, and their differences from natural DOC[J]. Chemosphere, 302: 134869.
[54]	ZHANG X Q, LI Y, YE J, et al., 2022a. The spectral characteristics and cadmium complexation of soil dissolved organic matter in a wide range of forest lands[J]. Environmental Pollution, 299: 118834.
[55]	ZHANG X Y, SU C, LIU X Y, et al., 2020. Periodical changes of dissolved organic matter (DOM) properties induced by biochar application and its impact on downward migration of heavy metals under flood conditions[J]. Journal of Cleaner Production, 275: 123787.
[56]	ZHAO C, WANG C C, LI J Q, et al., 2017. Interactions between copper(II) and DOM in the urban stormwater runoff: Modeling and characterizations[J]. Environmental Technology, 39(1): 120-129.
[57]	ZHOU L, ZHOU L, WU H B, et al., 2024. Effects of applying biochar on soil cadmium immobilisation and cadmium pollution control in lettuce (Lactuca sativa L.)[J]. Agriculture, 14(7): 1068.
[58]	ZHU Y, YI B J, HU H Y, et al., 2020. The relationship of structure and organic matter adsorption characteristics by magnetic cattle manure biochar prepared at different pyrolysis temperatures[J]. Journal of Environmental Chemical Engineering, 8(5): 104112.
[59]	范行程, 葛俊杰, 谢越, 等, 2024. 蘑菇渣和稻秸堆肥中DOM与Cu²⁺的络合机制[J]. 生态与农村环境学报, 40(2): 285-292.
	FAN X C, GE J J, XIE Y, et al., 2024. The binding properties of Cu(II) onto dissolved organic matter from mushroom residue and rice straw compost[J]. Journal of Ecology and Rural Environment, 40(2): 285-292.
[60]	刘玉灿, 高中鲁, 徐心怡, 等, 2024. 钙改性水葫芦基生物炭吸附水中敌草隆的效能与机理[J]. 化工进展, 43(8): 4630-4641. DOI
	LIU Y C, GAO Z L, XU X Y, et al., 2024. Adsorption performance and mechanism of diuron from water by calcium-modified water hyacinth-based biochar[J]. Chemical Industry and Engineering Progress, 43(8): 4630-4641. DOI
[61]	袁冬海, 崔骏, 洪志强, 等, 2016. 白洋淀沉水植物腐解溶解性有机物与重金属的相互作用[J]. 环境工程学报, 10(5): 2184-2192.
	YUAN D H, CUI J, HONG Z Q, et al., 2016. Interaction between dissolved organic matter released by macrophyte decomposition and heavy metal in Lake Baiyangdian[J]. Chinese Journal of Environmental Engineering, 10(5): 2184-2192.
[62]	张威宇, 李伟峻, 刘玉玲, 等, 2024. 人工湿地中沉积物溶解性有机质与Cd结合机制——以株洲市某人工湿地为例[J]. 农业环境科学学报, 43(6): 1377-1388.
	ZHANG W Y, LI W J, LIU Y L, et al., 2024. Mechanism of binding between dissolved organic matter and cadmium in constructed wetland sediments: Taking a constructed wetland in Zhuzhou City as an example[J]. Journal of Agro-Environment Science, 43(6): 1377-1388.
[63]	赵雄威, 吴东明, 李勤奋, 等, 2022. 基于紫外-可见光光谱法研究长期不同施肥对砖红壤溶解性有机质化学性质的影响[J]. 光谱学与光谱分析, 42(10): 3210-3216.
	ZHAO X W, WU D M, LI Q F, et al., 2022. Response of dissolved organie matter chemical properties to long term different fertilization in latosol: Insight from ultraviolet-visible spectroscopy[J]. Spectroscopy and Spectral Analysis, 42(10): 3210-3216.

生物炭	基本理化性质				元素组成/%
生物炭	pH	灰分质量分数/%	比表面积/(m²·g⁻¹)	孔体积/(cm³·g⁻¹)	C	H	O	N	H/C	O/C	(O+N)/C
CaBC	6.80	21.07	4.36	0.012	53.08B	4.28A	19.95A	1.37B	0.97A	0.28A	0.30A
BC	7.55	21.02	3.08	0.010	57.84A	4.23A	16.67B	1.71A	0.88B	0.22B	0.24B

生物炭	基本理化性质				元素组成/%
生物炭	pH	灰分质量分数/%	比表面积/(m²·g⁻¹)	孔体积/(cm³·g⁻¹)	C	H	O	N	H/C	O/C	(O+N)/C
CaBC	6.80	21.07	4.36	0.012	53.08B	4.28A	19.95A	1.37B	0.97A	0.28A	0.30A
BC	7.55	21.02	3.08	0.010	57.84A	4.23A	16.67B	1.71A	0.88B	0.22B	0.24B

生物炭	准一级动力学 ln(Q_e−Q_t)=lnQ_e−K₁t			准二级动力学 t/Q_t=1/K₂×Q_e²+t/Q_e			Elovich Q_t=a+blnt
生物炭	Q_e/(mg·g⁻¹)	K₁/(mg·g⁻¹·h⁻¹)	r²	Q_e/(mg·g⁻¹)	K₂/(mg·g⁻¹·h⁻¹)	r²	a/(mg·g⁻¹)	b/(mg·g⁻¹·h⁻¹)	r²
CaBC	10.54	0.88	0.791	11.04	0.13	0.957	6.86	1.13	0.981
BC	3.78	0.61	0.735	4.34	0.15	0.892	1.84	0.69	0.999

生物炭	准一级动力学 ln(Q_e−Q_t)=lnQ_e−K₁t			准二级动力学 t/Q_t=1/K₂×Q_e²+t/Q_e			Elovich Q_t=a+blnt
生物炭	Q_e/(mg·g⁻¹)	K₁/(mg·g⁻¹·h⁻¹)	r²	Q_e/(mg·g⁻¹)	K₂/(mg·g⁻¹·h⁻¹)	r²	a/(mg·g⁻¹)	b/(mg·g⁻¹·h⁻¹)	r²
CaBC	10.54	0.88	0.791	11.04	0.13	0.957	6.86	1.13	0.981
BC	3.78	0.61	0.735	4.34	0.15	0.892	1.84	0.69	0.999

生物炭	时间	pH值	离子态钙质量浓度/ (mg·L⁻¹)	络合态钙质量浓度/ (mg·L⁻¹)
CaBC	1	6.00	160.90	175.79
CaBC	127	7.59	61.54	15.73
BC	1	6.73	6.27	2.13
BC	127	8.15	3.80	2.67

Effect of Calcium Modification on the Binding of Biochar-derived Dissolved Organic Matter with Cd(II)

钙改性对生物炭中溶解性有机质与Cd(Ⅱ)结合的影响

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Figures/Tables 11

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生物炭	t/h	BDOM荧光组分	lgK	f/%	r²
CaBC	1	C1	4.67	43.07	0.903
		C2	4.43	7.45	0.962
		C3	4.38	13.99	0.900
	127	C1	5.04	30.19	0.831
		C2	4.45	2.93	0.886
		C3	4.63	5.82	0.816
BC	1	C1	4.27	53.21	0.993
		C2	4.42	7.58	0.976
		C3	4.45	35.52	0.970
	127	C1	4.63	23.46	0.968
		C2	No	No	No
		C3	4.62	5.95	0.974

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