Distribution Characteristics and Influencing Factors of Enzyme Activities in Typical Farmland Soils

doi:10.16258/j.cnki.1674-5906.2021.08.010

Ecology and Environment ›› 2021, Vol. 30 ›› Issue (8): 1634-1641.DOI: 10.16258/j.cnki.1674-5906.2021.08.010

• Research Articles • Previous Articles Next Articles

Distribution Characteristics and Influencing Factors of Enzyme Activities in Typical Farmland Soils

LI Xin¹^,²(), CHEN Xiaohua², GU Hairong², QIAN Xiaoyong²^,^*(), SHEN Genxiang²^,^*(), ZHAO Qingjie², BAI Yujie²

1. East China University of Science and Technology, Shanghai 200237, China
2. Shanghai Academy of Environmental Sciences, Shanghai 200233, China

Received:2021-03-09 Online:2021-08-18 Published:2021-11-03
Contact: QIAN Xiaoyong,SHEN Genxiang

典型农田土壤酶活性分布特征及影响因素分析

李欣¹^,²(), 陈小华², 顾海蓉², 钱晓雍²^,^*(), 沈根祥²^,^*(), 赵庆节², 白玉杰²

1.华东理工大学资源与环境工程学院,上海 200237
2.上海市环境科学研究院,上海 200233

通讯作者: 钱晓雍,沈根祥
作者简介:李欣（1995年生）,女,硕士研究生,研究方向为农田土壤健康评价。E-mail: 18862394452@163.com
基金资助:
上海市科技兴农项目(沪农科推字(2020)第2-1号);上海市生态环境局科研项目(沪环科[2020]第11号)

Abstract

Abstract:

Soil enzymes have been considered as key soil components involved in organic matter decomposition and nutrient cycling. Their activity level could be regarded as important soil health indicator. It is important to identify the response of enzyme activities to different agricultural practices in evaluation of soil health status and establishment of sustainable land management mechanisms. Therefore, this study investigated the soil enzymes of catalase activity (S-CAT), urease activity (S-UE), hydrolase activity (FDA), neutral phosphatase activity (S-NP) and key soil environment factors. Three types of farmlands in Shanghai, i.e., orchard, plastic shed vegetable field and paddy field were chosen as case-study areas. This study conducted both on-site monitoring and lab analysis to explore the response relationships between the distribution characteristics of soil enzyme activities and the biotic/abiotic factors. Results showed that, soil enzyme activities differed significantly (P<0.05) among different farmland types. S-CAT increased following the order of plastic shed vegetable field<orchard<paddy field, S-UE following the order of plastic shed vegetable field<paddy field<orchard, FDA showed no significant difference (P>0.05), and S-NP following the order of paddy field<plastic shed vegetable field<orchard. The variation coefficient of the first three enzymes was greater than 16% as moderate variation, while S-NP had strong variation. Redundancy analysis (RDA) showed that, soil enzyme activities were influenced by soil total potassium (TK) (P=0.002), hydrolytic nitrogen (HN) (P=0.002), respiratory flux (SR) (P=0.002), pH (P=0.008), available phosphorus (AP) (P=0.016), total nitrogen (TN) (P=0.002), and cation exchange capacity (CEC) (P=0.040). Soil nutrients, SR, pH and CEC were the main factors affecting enzyme activities in farmland under general tillage patterns in Shanghai. Our results support the conclusion that when evaluating soil health of different farmland soil types, it is important to select the most appropriate enzyme activities as soil health indicators.

Key words: farmland, soil enzyme activity, RDA analysis, farmland use type, soil health

摘要：

土壤酶是参与有机物分解和养分循环的关键成分,其活性可以用作衡量土壤健康的指标。了解土壤酶活性对农田利用方式的响应,对评价土壤健康状况、建立可持续耕作管理模式具有重要意义。为探究不同农田生产方式下农田土壤酶活性分布及主要环境影响因素,以果园、蔬菜大棚、水田3种农田利用方式为研究对象,采用野外监测和室内分析相结合的方法,探讨了农田土壤中过氧化氢酶活性（S-CAT）、脲酶活性（S-UE）、水解酶活性（FDA）、中性磷酸酶活性（S-NP）分布特征与生物和非生物因子间的响应关系。结果表明,农田土壤酶活性因耕作管理方式不同,存在较大差异（P<0.05）。土壤过氧化氢酶活性表现为蔬菜大棚<果园<水田,土壤脲酶活性表现为蔬菜大棚<水田<果园,水解酶活性在不同耕作管理方式下无显著差异（P>0.05）,中性磷酸酶活性表现为水田<蔬菜大棚<果园。前3种土壤酶活性的变异系数大于16%,为中等变异,中性磷酸酶为强变异。冗余分析（RDA）表明,影响土壤酶活性的关键因子是土壤全钾TK（P=0.002）、水解性氮HN（P=0.002）、呼吸通量SR（P=0.002）、pH（P=0.008）、有效磷AP（P=0.016）、全氮TN（P=0.002）和阳离子交换量CEC（P=0.040）。在上海农田的主要耕作模式下,土壤养分、呼吸通量、pH和阳离子交换量是影响农田土壤酶活性的主要因素。因此,在对不同类型农田土壤进行土壤健康评价时,应合理选择针对性的酶活性指标。

关键词: 农田, 土壤酶活性, RDA分析, 农田利用方式, 土壤健康

CLC Number:

LI Xin, CHEN Xiaohua, GU Hairong, QIAN Xiaoyong, SHEN Genxiang, ZHAO Qingjie, BAI Yujie. Distribution Characteristics and Influencing Factors of Enzyme Activities in Typical Farmland Soils[J]. Ecology and Environment, 2021, 30(8): 1634-1641.

李欣, 陈小华, 顾海蓉, 钱晓雍, 沈根祥, 赵庆节, 白玉杰. 典型农田土壤酶活性分布特征及影响因素分析[J]. 生态环境学报, 2021, 30(8): 1634-1641.

Figures/Tables 7

References 38

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农田利用类型 Type of farmland use	样地 Sample	生产模式 Production model	肥料投入Fertilizer input quantity		翻耕次数 Number of tillage
农田利用类型 Type of farmland use	样地 Sample	生产模式 Production model	化肥 Chemical fertilizer/(kg∙hm^-2)	有机肥 Organic fertilizer/(kg∙hm^-2)	翻耕次数 Number of tillage
果园 Orchard	3	立架栽植 Rack cultivation	410-1500	7500-28800	0
蔬菜大棚 Plastic shed vegetable field	4	一年4—6茬 4-6 crops per year	859-1199	10645-59970	4-6
水田 Paddy field	3	水旱轮作 Water-dry rotation	262-324	7496-13493	1

农田利用类型 Type of farmland use	样地 Sample	生产模式 Production model	肥料投入Fertilizer input quantity		翻耕次数 Number of tillage
农田利用类型 Type of farmland use	样地 Sample	生产模式 Production model	化肥 Chemical fertilizer/(kg∙hm^-2)	有机肥 Organic fertilizer/(kg∙hm^-2)	翻耕次数 Number of tillage
果园 Orchard	3	立架栽植 Rack cultivation	410-1500	7500-28800	0
蔬菜大棚 Plastic shed vegetable field	4	一年4—6茬 4-6 crops per year	859-1199	10645-59970	4-6
水田 Paddy field	3	水旱轮作 Water-dry rotation	262-324	7496-13493	1

项目 Item	指标 Indicator	单位 Unit	果园 Orchard	蔬菜大棚 Plastic shed vegetable field	水田 Paddy field
理化性质 Physicochemical property	pH值 pH value (pH)	‒	7.08±0.91b	5.86±0.89c	7.86±0.09a
	有机质 Soil organic matter (SOM)	g∙kg^-1	35.8±15.3b	48.4±16.6a	24.4±1.8b
	阳离子交换量 Cation exchange capacity (CEC)	cmol(+)∙kg^-1	17.8±3.9a	17.8±2.7a	12.8±0.5b
	全盐量 Total salt content (TS)	g∙kg^-1	3.72±5.14a	3.17±2.29ab	0.52±0.14b
	全磷 Total phosphorus (TP)	g∙kg^-1	2.00±1.27a	2.31±0.36a	0.79±0.10b
	有效磷 Available phosphorus (AP)	mg∙kg^-1	320±261a	310±79a	23±11b
	总氮 Total nitrogen (TN)	g∙kg^-1	2.5±0.9b	3.27±0.97a	1.41±0.12c
	水解性氮 Hydrolysable nitrogen (HN)	mg∙kg^-1	445±464a	418±163a	99±5b
	全钾 Total potassium (TK)	g∙kg^-1	18.3±1.1a	17.7±0.7a	15.4±0.4b
	速效钾Available potassium (AK)	mg∙kg^-1	625±431a	618±375a	105±16b
	粘粒质量分数 w_{(<0.002mm, clay)}	%	28.7±0.61a	24.2±0.65a	24.3±1.0a
生物特性 Biological characteristic	呼吸通量 Soil respiration efflux (SR)	mg∙m^-2∙h^-1	1.59±0.5b	3.70±1.68a	2.05±0.71b
生物特性 Biological characteristic	孔平均颜色变化率 Average well color development (AWCD)	‒	0.33±0.08b	0.41±0.07b	0.56±0.61a

项目 Item	指标 Indicator	单位 Unit	果园 Orchard	蔬菜大棚 Plastic shed vegetable field	水田 Paddy field
理化性质 Physicochemical property	pH值 pH value (pH)	‒	7.08±0.91b	5.86±0.89c	7.86±0.09a
	有机质 Soil organic matter (SOM)	g∙kg^-1	35.8±15.3b	48.4±16.6a	24.4±1.8b
	阳离子交换量 Cation exchange capacity (CEC)	cmol(+)∙kg^-1	17.8±3.9a	17.8±2.7a	12.8±0.5b
	全盐量 Total salt content (TS)	g∙kg^-1	3.72±5.14a	3.17±2.29ab	0.52±0.14b
	全磷 Total phosphorus (TP)	g∙kg^-1	2.00±1.27a	2.31±0.36a	0.79±0.10b
	有效磷 Available phosphorus (AP)	mg∙kg^-1	320±261a	310±79a	23±11b
	总氮 Total nitrogen (TN)	g∙kg^-1	2.5±0.9b	3.27±0.97a	1.41±0.12c
	水解性氮 Hydrolysable nitrogen (HN)	mg∙kg^-1	445±464a	418±163a	99±5b
	全钾 Total potassium (TK)	g∙kg^-1	18.3±1.1a	17.7±0.7a	15.4±0.4b
	速效钾Available potassium (AK)	mg∙kg^-1	625±431a	618±375a	105±16b
	粘粒质量分数 w_{(<0.002mm, clay)}	%	28.7±0.61a	24.2±0.65a	24.3±1.0a
生物特性 Biological characteristic	呼吸通量 Soil respiration efflux (SR)	mg∙m^-2∙h^-1	1.59±0.5b	3.70±1.68a	2.05±0.71b
生物特性 Biological characteristic	孔平均颜色变化率 Average well color development (AWCD)	‒	0.33±0.08b	0.41±0.07b	0.56±0.61a

排序轴Axis	第Ⅰ轴Axis 1	第Ⅱ轴Axis 2	第Ⅲ轴Axis 3	第Ⅳ轴Axis 4
土壤酶活性特征解释量 Soil enzyme activities eigenvalues/%	50.7	20.4	7.5	1.6
土壤酶活性特征与环境因子相关性 Enzymes-environmental factors correlations	0.924	0.904	0.805	0.636
土壤酶活性特征累计解释量 Cumulative explained amount of soil enzyme activity characteristics/%	50.7	71.1	78.6	80.2
土壤酶活性特征-环境因子关系累计解释量 Cumulative percentage variance of enzymes-environmental factors/%	63.1	88.6	98.0	100.0
典范特征值 Sum of all eigenvalues	1.000
总特征值 Sum of all canonical eigenvalues	0.802

Distribution Characteristics and Influencing Factors of Enzyme Activities in Typical Farmland Soils

典型农田土壤酶活性分布特征及影响因素分析

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