Analysis of Rhizosphere Soil Microbial Community Structure and Diversity of Different Vegetation Types in Liaohe Estuary Wetland

doi:10.16258/j.cnki.1674-5906.2025.02.005

Ecology and Environment ›› 2025, Vol. 34 ›› Issue (2): 222-232.DOI: 10.16258/j.cnki.1674-5906.2025.02.005

• Research Article【Ecology】 • Previous Articles Next Articles

Analysis of Rhizosphere Soil Microbial Community Structure and Diversity of Different Vegetation Types in Liaohe Estuary Wetland

YUE Hangyu¹^,²(), GUO Chengjiu¹, SU Fangli¹^,³^,⁴^,⁵^,^*(), WEI Chao¹^,³^,⁴

1. College of Water Conservancy, Shenyang Agricultural University, Shenyang 110866, P. R. China
2. Jilin Institute of Soil and Water Conservation Research, Changchun 130033, P. R. China
3. Liaoning Panjin Wetland Ecosystem National Observation and Research Station, Panjin 124000, P. R. China
4. Liaoning National Positioning Observation and Research Station of Shuangtai Estuary Wetland Ecosystem, Panjin 124000, P. R. China
5. Liaoning Provincial Key Laboratory of Soil Erosion Control and Ecological Restoration, Shenyang 110866, P. R. China

Received:2023-03-24 Online:2025-02-18 Published:2025-03-03
Contact: SU Fangli

辽河口湿地不同植被类型根际土壤微生物群落结构及多样性分析

岳航宇¹^,²(), 郭成久¹, 苏芳莉¹^,³^,⁴^,⁵^,^*(), 魏超¹^,³^,⁴

1.沈阳农业大学水利学院，辽宁沈阳 110866
2.吉林省水土保持科学研究院，吉林长春 130033
3.辽宁盘锦湿地生态系统国家野外科学观测研究站，辽宁盘锦 124000
4.辽宁双台河口湿地生态系统国家定位观测研究站，辽宁盘锦 124000
5.辽宁省水土流失与生态修复重点实验室，辽宁沈阳 110866

通讯作者: 苏芳莉
作者简介:岳航宇（1998年生），男，助理工程师，主要研究方向为水土保持与水生态研究。E-mail: yhy872437876@163.com
基金资助:
国家重点研发计划项目(2022YFF1301004);国家自然科学基金（青年）项目(32001370);辽宁省教育厅项目(JYTPT2024001)

Abstract

Abstract:

Rhizosphere microbial communities play a crucial role in wetland ecosystems by interacting with plant roots and influencing various aspects of plant growth, including nutrient uptake, resistance to environmental stressors, and the overall ecosystem function. These communities are complex and dynamic, driven by interactions between microorganisms and plants, and further influenced by soil physicochemical properties and environmental conditions. The rhizosphere is a hotspot for microbial activity, where microbes help plants acquire nutrients, degrade organic matter, and provide resistance to pathogens. Understanding the diversity, structure, and functional roles of these microbial communities across different wetland types is essential to understand the ecological processes that support wetland biodiversity, ecosystem stability, and resilience. Wetlands are valuable ecosystems that provide essential services such as carbon sequestration, water filtration, and habitat for biodiversity. In coastal wetlands where plants such as Phragmites australis and Suaeda salsa are commonly found, rhizosphere microbial communities are integral to the health of these ecosystems. Investigating the diversity and structure of these microbial communities across various wetland types, along with identifying the key factors that influence them, is crucial for understanding how these ecosystems function and are conserved in the face of ongoing environmental changes. The Liaohe Estuary Wetland, located in northern China, was selected as the research area for this study. This wetland encompasses a range of vegetation zones with distinct ecological characteristics. Soil samples were collected from five different wetland types: the Phragmites australis distribution zone (P. australis rhizosphere soil, D1), the interlaced P. australis and S. salsa zone (P. australis rhizosphere soil, D2), the interlaced P. australis and S. salsa zone (S. salsa rhizosphere soil, D3), the S. salsa distribution zone (S. salsa rhizosphere soil, D4), and tidal flat sediments (D5). These zones were selected based on their distinct vegetation types and potential to represent a variety of wetland conditions. To assess the microbial diversity and community structure in these soils, Illumina MiSeq PE300 high-throughput sequencing technology was employed based on 16S rRNA gene sequencing. This method is widely used in microbial ecology to obtain a comprehensive understanding of microbial community composition as it provides detailed taxonomic information at the species level. The sequencing data allowed for a detailed analysis of the microbial communities in the rhizosphere soils and a subsequent comparison of these communities across different wetland types. The results of this study revealed that different vegetation types significantly affected the physicochemical properties of the soil as well as the structure and function of the rhizosphere microbial communities. Soil samples from D1, which were dominated by P. australis, had a significantly higher organic matter content than the other samples. Conversely, soil from D5, which consisted of tidal flat sediments, exhibited a significantly higher total nitrogen content. These differences in soil properties are likely to influence the microbial community composition and functional potential, as different microbial groups may be adapted to thrive under specific environmental conditions. A total of 40 phyla, 95 classes, 184 orders, 311 families, 528 genera, and 888 species of soil microbes were identified across the rhizosphere soils of the five vegetation types in the Liaohe Estuary Wetland. These findings highlight the vast microbial diversity present in wetland rhizospheres. Among the identified phyla, Proteobacteria, Bacteroidetes, Actinobacteria, and Chloroflexi were dominant in all soil samples. Notably, Proteobacteria exhibited a significantly higher abundance than other phyla, making it the most dominant phylum in all samples. This is consistent with previous studies in other wetland ecosystems, where Proteobacteria have often been identified as a dominant group owing to their broad metabolic capabilities and adaptability to various environmental conditions. At the class level, microbial communities displayed greater specificity. For example, β-Proteobacteria were the dominant class in D1, whereas γ-Proteobacteria were dominant in the other four samples. This variation in microbial community composition at the class level suggests that environmental factors, such as soil moisture content, nutrient availability, and plant root exudates, can shape the microbial community structure in the rhizosphere. The relative abundances of the dominant and sub-dominant classes were relatively consistent across the samples, indicating a balanced microbial community without a clear absolute dominant class. This observation highlights the complexity of microbial interactions in the rhizosphere, where multiple groups may coexist and contribute to ecosystem function. No significant correlation was found between soil environmental factors and microbial diversity or richness across different wetland types. However, nitrite nitrogen content exhibited a stronger correlation with microbial diversity, whereas organic matter content was more strongly correlated with microbial richness. These results showed that microbial diversity and richness are influenced by a combination of factors; some environmental variables, such as nitrogen availability and organic matter, may play more significant roles in shaping the microbial community structure. This is consistent with the known role of nitrogen as a key nutrient for microbial growth and metabolism in soil environments. Redundancy analysis revealed that total nitrogen, organic matter, and chloride ion content were the primary factors influencing the microbial community structure in rhizosphere soils. These factors are crucial for the growth and development of microorganisms because they provide essential nutrients and affect the physical and chemical properties of the soil, which in turn affect microbial activity. Further analysis of soil environmental factors and their impact on the distribution of dominant microbial phyla indicated that microbial communities respond differently to environmental conditions. For example, increases in soil water content, oxidation-reduction potential, organic matter, and nitrite-nitrogen content enhance the availability of nutrients and moisture, which are necessary for microbial growth and development. These factors were found to significantly affect the microbial community structure, leading to changes in microbial composition and functional diversity. This underscores the importance of environmental factors in shaping the microbial communities in coastal wetland ecosystems. The findings of this study provide valuable insights into the ecological functions of the rhizosphere microbial communities in wetland ecosystems. The results demonstrate that vegetation type and soil physicochemical properties play critical roles in shaping microbial community structure and function. By understanding the factors influencing microbial communities, we can better understand the ecological processes that sustain wetland biodiversity and ecosystem stability. This knowledge is crucial for the conservation and management of coastal wetlands in northern China as it provides a theoretical foundation for maintaining ecological integrity and resilience in the face of environmental changes.

Key words: Liaohe Estuary Wetland, vegetation types, high-throughput sequencing, soil microbial, microbial diversity, community structure

摘要：

湿地是重要生态系统，微生物对维持其生态稳定发挥着重要作用。了解辽河口湿地不同植被类型根际土壤微生物群落结构和多样性特征对于揭示该区域植被与土壤的相互作用具有重要科学意义。以辽河口湿地为研究对象，选取芦苇分布区-芦苇根际土壤（D1）、芦苇-盐地碱蓬交错区-芦苇根际土壤（D2）、芦苇-盐地碱蓬交错区-盐地碱蓬根际土壤（D3）、盐地碱蓬分布区-盐地碱蓬根际土壤（D4）以及潮滩沉积物（D5），利用Illumina Miseq PE300高通量测序技术进行16S rRNA基因测序分析不同植被类型根际土壤微生物的多样性和群落结构特征，并探究土壤理化因子与其之间的关系。研究结果表明，1）辽河口湿地根际微生物检测出40门，95纲，184目，311科，528属，888种。2）在门水平上，变形菌门是最优势菌群。3）湿地各采样区域土壤微生物群落多样性分析表明，盐地碱蓬分布区-盐地碱蓬根际土壤微生物群落丰富度和多样性最高。4）冗余分析结果表明，土壤总氮、有机质、氯离子含量是影响土壤微生物群落结构的主要因素。研究成果可从微生态学角度为中国北方滨海湿地生态系统功能的研究和保护提供参考。

关键词: 辽河口湿地, 植被类型, 高通量测序, 土壤微生物, 微生物多样性, 群落结构

CLC Number:

X154.36
X172

YUE Hangyu, GUO Chengjiu, SU Fangli, WEI Chao. Analysis of Rhizosphere Soil Microbial Community Structure and Diversity of Different Vegetation Types in Liaohe Estuary Wetland[J]. Ecology and Environment, 2025, 34(2): 222-232.

岳航宇, 郭成久, 苏芳莉, 魏超. 辽河口湿地不同植被类型根际土壤微生物群落结构及多样性分析[J]. 生态环境学报, 2025, 34(2): 222-232.

Figures/Tables 10

Figure 1 Sampling point layout

Table 1 Main physicochemical properties of rhizosphere soil in different vegetation types

样品	pH	w_(水分)/%	氧化还原电位（ORP）/mV	w_(NO2−)/(mg∙kg⁻¹)	w_(TN)/(mg∙kg⁻¹)	w_(SOM)/(mg∙kg⁻¹)	w_(Cl−)/(mg∙kg⁻¹)
D1	7.79±0.21a	24.36±2.35a	0.91±0.10a	0.01±0.01a	236.53±33.47b	25.86±4.61a	2.84±0.92a
D2	7.71±0.34a	20.11±4.87a	1.54±0.17a	0.02±0.01a	232.04±25.86b	15.56±2.99b	3.12±1.27a
D3	8.09±0.53a	30.67±1.26a	1.59±0.05a	0.02±0.02a	248.52±99.56b	16.89±4.11b	2.84±0.54a
D4	7.82±0.29a	24.79±2.92a	1.37±0.12a	0.03±0.02a	939.57±161.56b	15.17±3.81b	4.11±0.73a
D5	7.81±0.15a	21.22±5.58a	0.42±0.08a	0.02±0.01a	1514.50±203.42a	15.51±2.63b	5.86±1.71a

Figure 2 Rarefaction curve of microbial communities in rhizosphere soil of different vegetation types

Figure 3 Composition of rhizosphere soil microbial OTU level in different vegetation types

Table 2 α-diversity of different vegetation types rhizosphere soil

样品	操作分类单元	Shannon 指数	Simpson 指数	ACE 指数	Chao1 指数	Coverage 指数
D1	661	5.133	0.0187	730.541	724.424	0.9969
D2	1427	6.154	0.0058	1511.769	1528.162	0.9944
D3	1313	5.775	0.0134	1418.338	1426.940	0.9938
D4	1448	6.154	0.0053	1541.361	1580.346	0.9938
D5	1319	5.884	0.0086	1429.491	1447.250	0.9937

Figure 4 Soil microbial community composition in rhizosphere soil of different vegetation types

Figure 5 Heatmap analysis of microbial gene level in rhizosphere soil of different vegetation types

Figure 6 Correlation analysis of soil environmental factors

Figure 7 Redundancy analysis of the relationship between soil microbial community structure and soil environmental factors on phylum level

Figure 8 Correlation analysis between soil microbial community structure and soil environmental factors in rhizosphere soil of different vegetation types on phylum level

References 44

[1]	ALAM M Z, BAKI BHUIYAN M A, ABDULLAH H M, et al., 2021. Changes of land use and land cover with the diversity of fishes, aquatic plants, and bird’s species at wetland ecosystem[J]. The Scientific World Journal, 2021: 7533119.
[2]	AN J X, LIU C, WANG Q, et al., 2019. Soil bacterial community structure in Chinese wetlands[J]. Geoderma, 337(2): 290-299.
[3]	SAN MIGUEL A, ROY J, GURY J, et al., 2014. Effects of organochlorines on microbial diversity and community structure in Phragmites australis rhizosphere[J]. Applied Microbiology and Biotechnology, 98: 4257-4266.
[4]	CHAPARRO J, BADRI D, VIVANCO J, 2013. Rhizosphere microbiome assemblage is affected by plant development[J]. The International Society for Microbial Ecology Journal, 8: 790-803.
[5]	CHEN T, HU W G, HE S B, et al., 2021a. Diversity and community structure of ammonia-oxidizing archaea in rhizosphere soil of four plant groups in Ebinur Lake Wetland[J]. Canadian Journal of Microbiology, 67(4): 271-280.
[6]	CHEN L, WANG Z W, DU S, et al., 2021b. Antimicrobial activity and functional genes of Actinobacteria from coastal wetland[J]. Current Microbiology, 78: 3058-3067.
[7]	COSTELLO E K, SCHMIDT S K, 2006. Microbial diversity in alpine tundra wet meadow soil: Novel Chloroflexi from a cold, water-saturated environment[J]. Environmental Microbiology, 8(8): 1471-1486. PMID
[8]	DAI T J, ZHANG Y, TANG Y S, et al., 2016. Identifying the key taxonomic categories that characterize microbial community diversity using full-scale classification: A case study of microbial communities in the sediments of Hangzhou Bay[J]. Federation of European Microbiological Societies Microbiology Ecology, 92(10): fiw150.
[9]	HUANG W, CHEN X, WANG K, et al., 2019. Comparison among the microbial communities in the lake, lake wetland, and estuary sediments of a plain river network[J]. Microbiology Open, 8(2): e00644.
[10]	KIRCHMAN D L, 2008. Microbial ecology of the oceans[M]. Second Edition. Hoboken: John Wiley & Sons, Inc.: 45-90.
[45]	赵美训, 丁杨, 于蒙, 2017. 中国边缘海沉积有机质来源及其碳汇意义[J]. 中国海洋大学学报(自然科学版), 47(9): 70-76.
	ZHAO M X, DING Y, YU M et al., 2017. Sources of sedimentary organic matter in China marginal sea surface sediments and implications of carbon sink[J]. Periodical of Ocean University of China, 47(9): 70-76.
[11]	LIM J M, KIM S J, HAMADA M, et al., 2014. Oryzihumus terrae sp. nov., isolated from soil and emended description of the genus Oryzihumus[J]. International Journal of Systematic and Evolutionary Microbiology, 64(Part 7): 2395-2399.
[12]	LIU F D, MO X, KONG W J, et al., 2020. Soil bacterial diversity, structure, and function of Suaeda salsa in rhizosphere and non-rhizosphere soils in various habitats in the Yellow River Delta, China[J]. Science of the Total Environment, 740: 140-144.
[13]	MITRA D, MONDAL R, KHOSHRU B, et al., 2022. Actinobacteria- enhanced plant growth, nutrient acquisition, and crop protection: Advances in soil, plant, and microbial multifactorial interactions[J]. Pedosphere, 32(1): 149-170.
[14]	NARSING RAO M P, LUO Z H, DONG Z Y, et al., 2022. Metagenomic analysis further extends the role of Chloroflexi in fundamental biogeochemical cycles[J]. Environmental Research, 209: 112888.
[15]	ODEDISHEMI AJIBADE F, WANG H C, GUADIE A, et al., 2021. Total nitrogen removal in biochar amended non-aerated vertical flow constructed wetlands for secondary wastewater effluent with low C/N ratio: Microbial community structure and dissolved organic carbon release conditions[J]. Bioresource Technology, 322: 124430.
[16]	WEI C, SU F L, YUE H Y, et al., 2024. Spatial distribution characteristics of denitrification functional genes and the environmental drivers in Liaohe Estuary Wetland[J]. Environmental Science and Pollution Research, 31(1): 1064-1078.
[17]	WU Y N, XU N, WANG H, et al., 2021. Variations in the diversity of the soil microbial community and structure under various categories of degraded wetland in Sanjiang Plain, northeastern China[J]. Land Degradation & Development, 32: 2143-2156.
[18]	YIN X B, WANG W T, WANG A H, et al., 2022. Microbial community structure and metabolic potential in the coastal sediments around the Yellow River Estuary[J]. Science of the Total Environment, 816(2): 151582.
[19]	陈登稳, 王孝国, 胡文革, 等, 2012. 艾比湖湿地氨氧化细菌数量空间分布及其与土壤环境相关性分析[J]. 微生物学通报, 39(3): 334-343.
	CHEN D W, WANG X G, HU W G, et al., 2012. Correlation analysis between the distribution of ammonia-oxidizing bacteria and soil environment in Ebinur Lake Wetland[J]. Microbiology China, 39(3): 334-343.
[20]	洪志锋, 张旎晨, 阿丹, 等, 2022. 共代谢作用下芦苇根际细菌多样性与群落组成[J]. 中国环境科学, 42(4): 1812-1818.
	HONG Z F, ZHANG N C, A D, et al., 2022. Bacterial diversity and community composition in the Phragmites australis rhizosphere by cometabolism[J]. China Environmental Science, 42(4): 1812-1818.
[21]	黄俊杰, 陆雅海, 2022. 土壤拟杆菌与梭菌分解多糖类有机物质的研究进展与展望[J]. 微生物学通报, 49(3): 1147-1157.
	HUANG J J, LU Y H, 2022. Decomposition of soil polymeric organic matter by Bacteroidetes and Clostridia: Progress and perspectives[J]. Microbiology China, 49(3): 1147-1157.
[22]	李金业, 陈庆锋, 李青, 等, 2021. 黄河三角洲滨海湿地微生物多样性及其驱动因子[J]. 生态学报, 41(15): 6103-6114.
	LI J Y, CHEN Q F, LI Q, et al., 2021. Analysis of microbial diversity and driving factors in coastal wetlands of the Yellow River Delta[J]. Acta Ecologica Sinica, 41(15): 6103-6114.
[23]	李善家, 王福祥, 从文倩, 等, 2022. 河西走廊荒漠土壤微生物群落结构及环境响应[J]. 土壤学报, 59(6): 1718-1728.
	LI S J, WANG F X, CONG W Q, et al., 2022. Microbial community structure and environmental response of desert soil in Hexi Corridor[J]. Acta Pedologica Sinica, 59(6): 1718-1728.
[24]	林少颖, 陈桂香, 刘旭阳, 等, 2020. 互花米草入侵对河口湿地土壤细菌群落结构及多样性影响[J]. 环境科学学报, 40(8): 3001-3012.
	LIN S Y, CHEN G X, LIU X Y, et al., 2020. Effects of Spartina alterniflora invasion on soil bacterial community structure and diversity in estuarine wetland[J]. Acta Scientiae Circumstantiae, 40(8): 3001-3012.
[25]	么秀颖, 闫丹丹, 戚丽萍, 等, 2022. 中国湿地生态系统碳库对环境变化的响应分析[J]. 环境科学学报, 42(1): 111-120.
	YAO X Y, YAN D D, QI L P, et al., 2022. Responses of wetland ecosystem carbon pools to multiple environmental change drivers in China[J]. Acta Scientiae Circumstantiae, 42(1): 111-120.
[26]	孟妍君, 秦鹏, 2020. 珠江三角洲滨海湿地土壤微生物群落多样性与养分的耦合关系[J]. 水土保持研究, 27(6): 77-84.
	MENG Y J, QIN P, 2020. Coupling relationship between microbial community diversity and soilnutrients in different wetlands in coastal area of Pearl River Delta[J]. Research of Soil and Water Conservation, 27(6): 77-84.
[27]	裴希超, 许艳丽, 魏巍, 2009. 湿地生态系统土壤微生物研究进展[J]. 湿地科学, 7(2): 181-186.
	PEI X C, XU Y L, WEI W, 2020. A review on soil microorganisms in wetland ecosystem[J]. Wetland Science, 7(2): 181-186.
[28]	钱凤魁, 周阳, 李婉宁, 等, 2021. 辽河口翅碱蓬湿地退化区土壤理化性质及生态阈值分析[J]. 土壤通报, 52(5): 1085-1094.
	QIAN F K, ZHOU W, LI W N, et al., 2021. Soil characteristics in wetland degradation areas and soil threshold calculation for Suaeda Salsa growth in Liaohe Estuary Wetland[J]. Chinese Journal of Soil Science, 52(5): 1085-1094.
[29]	沈琦, 郝雅荞, 徐潇航, 等, 2020. 基于高通量测序技术的盐地碱蓬根际细菌群落多样性分析[J]. 浙江理工大学学报(自然科学版), 43(5): 671-677.
	SHEN Q, HAO Y Q, XU X H, et al., 2020. Analysis of rhizosphere bacterial diversity in Suaeda glauca Bunge based on high-throughput sequencing[J]. Journal of Zhejiang Sci-Tech University, 43(5): 671-677.
[30]	孙建平, 刘雅辉, 左永梅, 等, 2020. 盐地碱蓬根际土壤细菌群落结构及其功能[J]. 中国生态农业学报(中英文), 28(10): 1618-1629.
	SUN J P, LIU Y H, ZUO Y M, et al., 2020. The bacterial community structure and function of Suaeda salsa rhizosphere soil[J]. Chinese Journal of Eco-Agriculture, 28(10): 1618-1629.
[31]	唐旖, 2021. 九龙江口及台湾海峡沉积物中细菌和古菌群落结构分析[D]. 上海: 上海大学.
	TANG Y, 2021. Community structure analysis of bacteriaand archaea in sediments of the Jiulong River Estuary and the Taiwan Strait[D]. Shanghai: Shanghai University.
[32]	汪仲琼, 王为东, 祝贵兵, 等, 2011. 人工和天然湿地芦苇根际土壤细菌群落结构多样性的比较[J]. 生态学报, 31(16): 4489-4498.
	WANG Z Q, WANG W D, ZHU G B, et al., 2011. A comparative study on the diversity of rhizospheric bacteria community structure in constructed wetland and natural wetland with reed domination[J]. Acta Ecologica Sinica, 31(16): 4489-4498.
[33]	王露莹, 孙慧珍, 杨雪 2022. 松花江下游滨岸带典型植被根际土壤细菌群落结构与功能多样性[J]. 环境科学, 43(4): 2182-2191.
	WANG L Y, SUN H Z, YANG X, et al., 2022. Structure and functional diversity of bacterial community in rhizosphere soil of typical vegetation in the riparian zone along the downstream of Songhua River[J]. Environmental Science, 43(4): 2182-2191.
[34]	王娜, 高婕, 魏静, 等, 2019. 三江平原湿地开垦对土壤微生物群落结构的影响[J]. 环境科学, 40(5): 2375-2381.
	WANG N, GAO J, WEI J, et al., 2019. Effects of wetland reclamation on soil microbial community structure in the Sanjiang Plain[J]. Environmental Science, 40(5): 2375-2381.
[35]	王婷, 焦健, 李朝周, 等, 2021. 不同生境芦苇根际土壤性质与根系生理的比较研究[J]. 中国草地学报, 43(4): 78-86.
	WANG T, JIAO J, LI C Z, et al., 2021. Comparative study on rhizosphere soil properties and root physiology of reedin different habitats[J]. Chinese Journal of Grassland, 43(4): 78-86.
[36]	王巍琦, 李变变, 张军, 等, 2019. 干旱区不同类型盐碱土壤细菌群落多样性[J]. 干旱区研究, 36(5): 1202-1211.
	WANG W Q, LI B B, ZHANG J, et al., 2019. Diversity of bacterium communities in saline or alkaline soil in arid area[J]. Arid Zone Research, 36(5): 1202-1211.
[37]	吴胜男, 王晓锋, 刘婷婷, 等, 2022. 基于CiteSpace的湿地恢复研究进展[J]. 生态学报, 42(3): 1224-1239.
	WU S N, WANG X F, LIU T T, et al., 2022. The research progress of wetland restoration based on CiteSpace[J]. Acta Ecologica Sinica, 42(3): 1224-1239.
[38]	武晓倩, 范保硕, 滕叶文, 等, 2022. 白洋淀流域河岸带草本植物群落分布特征与土壤环境因子的关系[J]. 应用与环境生物学报, 28(6): 1608-1614.
	WU X Q, FAN B S, TENG Y W, et al., 2022. Relationship between herbaceous community distribution and soil environmental factors in the riparian zone of the Baiyangdian River Basin[J]. Chinese Journal of Applied and Environmental Biology, 28(6): 1608-1614.
[39]	鲜文东, 张潇橦, 李文均, 2020. 绿弯菌的研究现状及展望[J]. 微生物学报, 60(9): 1801-1820.
	XIAN W D, ZHANG X T, LI W J, 2020. Research status and prospect on bacterial phylum Chloroflexi[J]. Acta Microbiologica Sinica, 60(9): 1801-1820.
[40]	杨阳, 陈克龙, 章妮, 等, 2022. 青海湖流域高寒湿地土壤微生物群落对不同降水梯度的响应[J]. 应用与环境生物学报, 28(2): 290-299.
	YANG Y, CHEN K L, ZHANG N, et al., 2022. Responses of soil microbial community to different precipitation gradients in the alpine wetlands of Qinghai Lake Basin[J]. Chinese Journal of Applied and Environmental Biology, 28(2): 290-299.
[41]	张甘霖, 龚子同, 2012. 土壤调查实验室分析方法[M]. 北京: 北京出版社: 38-116.
	ZHANG G L, GONG Z T, 2012. Methods for laboratory analysis of soil investigation[M]. Beijing: Science Press: 38-116.
[42]	张攀, 谢先军, 黎清华, 等, 2022. 东寨港红树林沉积物中微生物群落结构特征及其对环境的响应[J]. 地球科学, 47(3): 1122-1135.
	ZHANG P, XIE X J, LI Q H, et al., 2022. Microbial community structure and its response to environment in mangrove sediments of Dongzhai Port[J]. Earth Science, 47(3): 1122-1135.
[43]	张鑫磊, 金锐, 杨镇, 等, 2019. 长江口崇明东滩湿地微生物群落结构研究[J]. 土壤通报, 50(5): 1178-1184.
	ZHANG X L, JIN R, YANG Z, et al., 2019. Microbial community structure in the Chongming Eastern Wetland of the Yangtze Estuary[J]. Chinese Journal of Soil Science, 50(5): 1178-1184.
[44]	赵华显, 阎冰, 徐悦, 等, 2020. 北部湾红树林沉积物中微生物群落结构的时空变化分析[J]. 基因组学与应用生物学, 39(5): 2161-2169.
	ZHAO H X, YAN B, XUE X, et al., 2020. Spatiotemporal analysis of microbial community structure in the mangrove sediments in Beibu Gulf[J]. Genomics and Applied Biology, 39(5): 2161-2169.

Analysis of Rhizosphere Soil Microbial Community Structure and Diversity of Different Vegetation Types in Liaohe Estuary Wetland

辽河口湿地不同植被类型根际土壤微生物群落结构及多样性分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 44

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Yujia, LU Mei, ZHAO Xuyan, FENG Jun, LIU Guoqing, GUO Chuxiao, WANG Mingliu, HUANG Minchao, CHEN Zhiming. Response of Soil Bacterial Community Structure to Nitrogen Addition in Degraded Napahai Alpine Meadow [J]. Ecology and Environment, 2025, 34(2): 233-246.
[2]	ZHANG Jinglei, WANG Guoliang, WU Bo, JIA Chunlin, ZHANG Jinhong, ZHOU Yuan, MA Bing. The Effects of Alfalfa-Triticale Rotation on Soil Bacterial and Fungal Community Diversity and Co-occurrence Network in Coastal Saline-Alkaline Soil [J]. Ecology and Environment, 2024, 33(7): 1048-1062.
[3]	LI Chengyang, LIANG Zhihui, LI Zhenming, CAI Min, XU Ruiyao, CHEN Xiuyu, DING Jiayin, XU Qiuyun, PENG Fei. Plant Community Characteristics and Soil Characteristics of Degraded Alpine Meadows in the Beilu River Basin of the Yangtze River Source Area [J]. Ecology and Environment, 2024, 33(7): 1063-1071.
[4]	JIANG Yunfeng, YAN Ting, LIU Junnan, MA Bingzeng, WANG Haimeng, DOU Xiaomeng. Responses of Soil Mesofauna in Agricultural Fields to the Frequency of Corn Stover Mulching in Northeastern China’s Black Soil Region [J]. Ecology and Environment, 2024, 33(5): 699-707.
[5]	CHEN Hongjie, LIAO Hongkai, LONG JIAN, ZHAO Yuxin, ZHAN Kaixian, RAN Taishan, YANG Guomei. Effects of Reductive Soil Disinfestation on Soil Protist Community [J]. Ecology and Environment, 2024, 33(4): 539-547.
[6]	SONG Jiangqin, YIN Yali, ZHAO Wen, LIU Yan, SUI Qiqi, HUO Jiuyan, ZHENG Wenxian, LI Shixiong. Characteristics of Spatial Differentiation of Soil Microbial Communities in Degraded Grassland on the “Black Soil Beaches” of Qinghai Plateau [J]. Ecology and Environment, 2024, 33(11): 1696-1707.
[7]	ZHANG Chuanguang, SHEN Yan, ZHANG Shanshan, LI Yuwen, CHEN Jian, YANG Wenzhong. Analysis of Microbial Diversity of Rhizosphere Soil of Pinus wangii (Pinaceae) in In Situ and Ex Situ Conservation [J]. Ecology and Environment, 2024, 33(10): 1544-1553.
[8]	LI Qing, ZHANG Mengyue, YU Mingqiao, LI Xiaoxuan, CHANG Ming, CHEN Libin, DING Sen. Community Structure and Influencing Factors of Macroinvertebrate in Urban Rivers of Dongguan [J]. Ecology and Environment, 2024, 33(1): 101-110.
[9]	LI Xun, ZHANG Yan, SONG Simeng, ZHOU Yang, ZHANG Jian. Bacterial Community Characteristics during the Mixed Decomposition of Litter from Pinus massoniana and Indigenous Broad-leaved Tree Species in Southwestern China [J]. Ecology and Environment, 2024, 33(1): 12-27.
[10]	TANG Zhiwei, WENG Ying, ZHU Xiatong, CAI Hongmei, DAI Wenci, WANG Pengna, ZHENG Baoqiang, LI Jincai, CHEN Xiang. Meta-analysis of Soil Microbial Mass Carbon and Its Influencing Factors in Farmland in China under Straw Return [J]. Ecology and Environment, 2023, 32(9): 1552-1562.
[11]	SONG Simeng, LIN Dongmei, ZHOU Hengyu, LUO Zongzhi, ZHANG Lili, YI Chao, LIN Hui, LIN Xingsheng, LIU Bin, SU Dewei, ZHENG Dan, YU Shikui, LIN Zhanxi. Effects of Planting Cenchrus fungigraminus on Plant Species Diversity and Soil Physicochemical Properties in the Ulan Buh Desert [J]. Ecology and Environment, 2023, 32(9): 1595-1605.
[12]	LIU Han, WANG Ping, SUN Luyuan, QING Wenjing, CHEN Xiaofen, CHEN Jin, ZHOU Guopeng, LIANG Ting, LIU Jia, LI Yanli. Effects of Winter Green Manure Planting on Soil Microbial Biomass Carbon, Nitrogen, and Enzyme Activity in Red Soil Young Citrus Orchard [J]. Ecology and Environment, 2023, 32(9): 1623-1631.
[13]	GU Meiying, TANG Guangmu, ZHANG Yunshu, HUANG Jian, ZHANG Zhidong, ZHANG Lijuan, ZHU Jing, TANG Qiyong, CHU Min, XU Wanli. Effects of Organic Fertilizers and Biochar on Microorganism Community Characteristics in Saline-alkali Sandy Soil of Xinjiang [J]. Ecology and Environment, 2023, 32(8): 1392-1404.
[14]	LIANG Chuan, YANG Yanfang, YU Shanshan, ZHOU Li, ZHANG Jingwei, ZHANG Xiujuan. Differences of Microbial Biomass and Community Structure Characteristics in Sediments under Net-pen and Pond Fish Farming [J]. Ecology and Environment, 2023, 32(8): 1487-1495.
[15]	CHEN Dongdong, HUO Lili, ZHAO Liang, CHEN Xin, SHU Min, HE Fuquan, ZHANG Yukun, ZHANG Li, LI Qi. Contribution of Water and Heat Factors to Spatial Variability of Soil Microbial Biomass Carbon and Nitrogen in Qinghai Alpine Grassland: Based on Enhanced Regression Tree Model [J]. Ecology and Environment, 2023, 32(7): 1207-1217.