微生物群落对河流底泥中锑含量变化的响应

doi:10.16258/j.cnki.1674-5906.2023.03.018

生态环境学报 ›› 2023, Vol. 32 ›› Issue (3): 609-618.DOI: 10.16258/j.cnki.1674-5906.2023.03.018

微生物群落对河流底泥中锑含量变化的响应

阳涅¹^,²(), 孙晓旭², 孔天乐¹^,², 孙蔚旻², 陈泉源¹, 高品¹^,²^,^*()

1.东华大学环境科学与工程学院，上海 201620
2.广东省科学院生态环境与土壤研究所，广东广州 510650

收稿日期:2023-01-16 出版日期:2023-03-18 发布日期:2023-06-02
通讯作者: *高品，E-mail: pingao@dhu.edu.cn
作者简介:阳涅（1999年生），男，硕士研究生，研究方向为金属污染环境下微生物的响应机制。E-mail: 1255948322@qq.com
基金资助:
国家自然科学基金项目(51978136);中央高校基本科研业务费专项(2232022A-10);广州市科技计划项目(201904010366);广东省科学院发展专项资金项目(2020GDASYL-20200102014);广东省科学院发展专项资金项目(2022GDASZH-2022010201);广东省科技计划项目(2020B1212060048)

Response of Microbial Communities to Changes in Antimony Pollution Concentrations in Fluvial Sediment

YANG Nie¹^,²(), SUN Xiaoxun², KONG Tianle¹^,², SUN Weimin², CHEN Quanyuan¹, GAO Pin¹^,²^,^*()

1. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, P. R. China
2. Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management/Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, P. R. China

Received:2023-01-16 Online:2023-03-18 Published:2023-06-02

摘要/Abstract

摘要：

锑（Sb）矿的开采会造成周边水环境发生Sb污染，对周边生态环境和人体健康造成潜在风险。微生物是河流中Sb污染物生物地球化学循环的主要驱动力之一，通过氧化/还原过程调控Sb的价态转化过程，从而改变Sb的毒性和流动性。以贵州独山县Sb污染河流底泥为研究对象，利用地球化学参数分析、高通量测序和统计分析等方法，研究Sb污染河流底泥中Sb的浓度分布规律及其对河流底泥微生物群落的选择性影响。结果表明，随着与Sb冶炼厂排水口距离的增加，河流底泥中Sb浓度逐渐降低，这可能是微生物还原产生的Sb(III) 在厌氧环境下易与铁锰化合物结合形成沉淀所导致的。基于随机森林分析发现，Sb是塑造河流底泥微生物群落结构的主要因素，河流底泥微生物多样性随Sb浓度的降低而呈现逐渐增加的变化趋势。通过LEfSe差异分析可知，Sb是河流底泥微生物群落结构组成演变的关键驱动因子，在不同Sb污染环境下富集了差异微生物。结合共现网络分析，发现栖泥沼杆菌属Paludibacter和糖发酵菌属Saccharofermentans是Sb污染河流底泥中的核心微生物，其相对丰度与Sb_tot、Sb(III) 浓度呈显著正相关，表明这些关键微生物对Sb生物地球化学循环发挥重要作用，尤其对调控Sb的还原过程具有重要潜力。

关键词: 锑, 河流底泥, 微生物群落, 关键微生物, 共现网络分析, 锑还原菌

Abstract:

With the development of our country's mining industry, antimony (Sb) mining process can cause Sb pollution in the surrounding water environments, posing potential risks to the surrounding ecological environment and human health. Microorganisms are one of the main driving forces of the biogeochemical cycle of Sb pollutants in rivers. They regulate the valence transformation process of Sb through oxidation/reduction process, thus changing the toxicity and mobility of Sb. In this paper, we took Sb polluted river sediment in Dushan County, Guizhou Province as the research object, and used the methods of geochemical parameter analysis, high-throughput sequencing and statistical analysis to study the distribution of Sb concentration in the river sediment and its selective effect on the river sediment microbial community. The results showed that the concentration of Sb in the river sediment gradually decreased with the increase of the distance from the sewage outlet of Sb smelting plant, which might be caused by the fact that Sb (III) produced by microbial reduction was easy to combine with iron and manganese compounds and precipitate under anaerobic environment. Based on the random forest analysis, it was found that Sb was the main factor shaping the microbial community structure of river sediment, and the microbial diversity of river sediment showed a gradual increasing trend with the decrease of Sb concentration. According to LEfSe difference analysis, Sb was the key driving factor of microbial community composition evolution in river sediment. Different microorganisms were enriched in different Sb polluted environments. Combined with co-occurrence network analysis, it was found that Paludibacter and Saccharofermentans were the keystone in Sb polluted river sediment. The relative abundance was significantly and positively correlated with the concentrations of Sb_tot and Sb(III), suggesting that these key microorganisms played an important role in the biogeochemical cycle of Sb, especially in regulating the reduction process of Sb.

Key words: antimony, river sediment, keystone, microbial community, co-occurrence network analysis, Antimony reducing bacteria

中图分类号:

X171.5
X172

阳涅, 孙晓旭, 孔天乐, 孙蔚旻, 陈泉源, 高品. 微生物群落对河流底泥中锑含量变化的响应[J]. 生态环境学报, 2023, 32(3): 609-618.

YANG Nie, SUN Xiaoxun, KONG Tianle, SUN Weimin, CHEN Quanyuan, GAO Pin. Response of Microbial Communities to Changes in Antimony Pollution Concentrations in Fluvial Sediment[J]. Ecology and Environment, 2023, 32(3): 609-618.

图/表 9

图1 采样点示意图

Figure 1 Schematic diagram of sampling points

图2 不同区域河流底泥中环境因子差异

Figure 2 Differences in environmental factors in river sediment in different regions

图3 不同区域下河流底泥微生物多样性对比

Figure 3 Comparison of microbial diversity in river sediment in different regions

图4 区域A中属一级相对丰度前10的微生物

Figure 4 Top 10 microorganisms with first order relative abundance in region A

图5 相对丰度前50中不同位置河流底泥差异微生物

Figure 5 Differences in microorganisms in river sediment at different positions in the top 50 relative abundances

图6 河流底泥微生物互作网络分析与关键微生物的鉴定

Figure 6 Analysis of microbial Interaction network in river sediment and identification of keystone

图7 环境因子与微生物群落丰富度的关系

Figure 7 The relationship between environmental factors and microbial community richness

图8 环境因子与微生物互作网络分析

Figure 8 Analysis of the interaction network between environmental factors and microorganisms

图9 不同区域河流底泥中关键微生物功能预测

Figure 9 Prediction of key microbial functions in river sediment at different locations

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微生物群落对河流底泥中锑含量变化的响应

Response of Microbial Communities to Changes in Antimony Pollution Concentrations in Fluvial Sediment

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