Correlation Analysis of the Occurrence of Bacterial Wilt and Physicochemical Properties and Enzyme Activity of Root-Zone Soil of Casuarina spp.

doi:10.16258/j.cnki.1674-5906.2022.01.009

Ecology and Environment ›› 2022, Vol. 31 ›› Issue (1): 70-78.DOI: 10.16258/j.cnki.1674-5906.2022.01.009

• Research Articles • Previous Articles Next Articles

Correlation Analysis of the Occurrence of Bacterial Wilt and Physicochemical Properties and Enzyme Activity of Root-Zone Soil of Casuarina spp.

SUN Zhan¹^,²(), WANG Shengjie¹^*(), YANG Jinchang¹, WEI Yongcheng¹, LIN Chunhua², MA Haibin¹^,^**()

1. Key Laboratory of State Forestry and Prairie Administration on Tropical Forestry Research/Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, P. R. China
2. Hainan University College of Plant Protection, Haikou 570228, P. R. China

Received:2021-11-01 Online:2022-01-18 Published:2022-03-10
Contact: MA Haibin

木麻黄根区土壤理化特性及酶活性与青枯病发生关联分析

孙战¹^,²(), 王圣洁¹^*(), 杨锦昌¹, 魏永成¹, 林春花², 马海宾¹^,^**()

1.中国林业科学研究院热带林业研究所/热带林业研究国家林业和草原局重点实验室,广东广州 510520
2.海南大学植物保护学院,海南海口 570228

通讯作者: 马海宾
作者简介:孙战（1994年生）,男,硕士,主要从事植物保护研究。E-mail: damonzhan10@163.com;
王圣洁（1989年生）,男,助理研究员,博士,主要从事植物病理研究。E-mail: ritfwsj@caf.ac.cn第一联系人：*孙战与王圣洁对本工作具有同等贡献,共享第一作者
基金资助:
广东省林业科技创新项目(2018KJCX010);广东省地方标准制（修）订林业项目(2016-DB-03)

Abstract

Abstract:

In order to investigate the relationship between the occurrence of bacterial wilt in Casuarina spp. forest and soil physicochemical properties and enzymatic activity, this study selected diseased and healthy plants of Casuarina spp. from Dacheng Town, Raoping County, Chaozhou City of Guangdong Province, Tangwei Town, Wuchuan City and Qianshan Town, Xuwen County of Zhanjiang City. A total of 54 soil samples were collected and the physicochemical properties and enzyme activity in the soil were measured. The results showed that the disease index was 1.33 in Raoping, 5.47 in Wuchuan, and 10.53 in Xuwen. As part of the data analysis, the three sampling plots were classified according to the disease index, from light to severe, as Raoping plot (level 1), Wuchuan plot (level 2), and Xuwen plot (level 3). Soil pH value, SOM, TN, TP, TK, AN, AP, and AK content in the root-zone of diseased plants of Casuarina spp. were lower than those of healthy plants. There were significant differences in TN contents (P<0.05), and the differences in SOM, AP, TK, AK and AN contents were highly significant (P<0.01). The contents of URE, ACP, CAT, POD and PPO in the roots of healthy plants of Casuarina spp. were higher than those in diseased plants. The the contents of INV in the root had highly significant negative correlations with the TK and AP content in the soil (P<0.01), and highly significant positive correlation with the content of PPO (P<0.01), and negatively correlations with ACP, INV, CAT, and POD activity. The soil PPO activity of different disease degrees were as follows: level 1 (23.45 mg∙g^-1∙h^-1), level 2 (30.43 mg∙g^-1∙h^-1), and level 3 (59.63 mg∙g^-1∙h^-1). In summary, PPO activity can be used as an indicator of the degree of bacterial wilt infection of Casuarina spp.. The mechanism of soil physicochemical properties and enzymatic activity against bacterial wilt is complex and thus needs to be further investigated. Future studies may prove it at the molecular and physiological levels, explore its optimal concentration to resist bacterial wilt, take into account other elements, and examine combined effects of multiple elements. From the perspective of soil microbes, future research should examine the prevention of plant diseases in collaboration with biocontrol bacteria.

Key words: Casuarina spp., bacterial wilt, disease degree, physicochemical properties, enzyme activity

摘要：

为探究木麻黄（Casuarina spp.）根区土壤理化特性及酶活性与青枯病发生的关系,进而为加强生物防治提供参考。选取位于广东省潮州市饶平县大埕镇、湛江市吴川市塘尾镇和湛江市徐闻县前山镇的3个木麻黄人工林为研究对象,在每个林分下分别设置3个样地,每个样地采集木麻黄青枯病感病植株和健康植株根区土壤各3份,共54个土壤样品,并测定部分土壤理化指标和酶活性。结果表明,饶平样地感病指数为1.33,吴川样地为5.47,徐闻样地为10.53。为了便于分析,根据感病指数将这3个研究样地进行青枯病发病等级的人为划分,由轻及重划分为饶平样地（1级）、吴川样地（2级）和徐闻样地（3级）。木麻黄青枯病感病植株根区土壤pH,土壤有机质（SOM）、全氮（TN）、全磷（TP）、全钾（TK）含量以及碱解氮（AN）、有效磷（AP）、速效钾（AK）含量均较健康植株低,且TN含量差异显著（P<0.05）,SOM、AP、TK、AK和AN含量差异极显著（P<0.01）。木麻黄健康植株根区土壤脲酶（URE）、酸性磷酸酶（ACP）、过氧化氢酶（CAT）、过氧化物酶（POD）以及多酚氧化酶（PPO）活性均高于感病植株;而蔗糖酶（INV）活性低于感病植株。此外,木麻黄青枯病发病等级与土壤中TK含量和AP含量呈极显著负相关关系（P<0.01）,与PPO活性呈极显著正相关关系（P<0.01）,与ACP、INV、CAT、POD活性均呈负相关关系。不同发病等级土壤PPO活性分别为：1级23.45 mg∙g^-1∙h^-1、2级30.43 mg∙g^-1∙h^-1、3级59.63 mg∙g^-1∙h^-1。综上,PPO活性可以作为反映木麻黄感染青枯病程度的酶学指标。土壤理化特性和酶活性对木麻黄起到的抗病机理是复杂的,仍需进一步从分子和生理水平上进行研究和考证;探究其抵御木麻黄青枯病的最适浓度,研究与其他元素的作用,将多种元素结合协同发挥作用。从土壤微生物角度,与生防菌协同预防植物病害的发生是未来可研究的方向。

关键词: 木麻黄, 青枯病, 发病等级, 理化特性, 酶活性

CLC Number:

S718.5
X173

SUN Zhan, WANG Shengjie, YANG Jinchang, WEI Yongcheng, LIN Chunhua, MA Haibin. Correlation Analysis of the Occurrence of Bacterial Wilt and Physicochemical Properties and Enzyme Activity of Root-Zone Soil of Casuarina spp.[J]. Ecology and Environment, 2022, 31(1): 70-78.

孙战, 王圣洁, 杨锦昌, 魏永成, 林春花, 马海宾. 木麻黄根区土壤理化特性及酶活性与青枯病发生关联分析[J]. 生态环境学报, 2022, 31(1): 70-78.

Figures/Tables 8

References 35

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采样区Sampling plots	经纬度 Latitude and longitude	气候 Climate	年均降雨量 The average annual rainfall/mm	年平均气温 The annual average temperature/℃	土壤类型 Soil types	pH	林分类型 Stand types	林龄 Age of forest/ a
RP	23°35′20″N, 117°7′22″E	亚热带海洋性季风	1745.9	21.4	滨海潮沙土	5.90	纯林	8
WC	21°21′55″N, 110°43′23″E	亚热带季风	1597.8	22.5	滨海潮沙土	6.46	纯林	6
XW	20°28′7″N, 110°31′20″E	热带季风	1400	23.6	滨海潮沙土	5.16	纯林	7

采样区Sampling plots	经纬度 Latitude and longitude	气候 Climate	年均降雨量 The average annual rainfall/mm	年平均气温 The annual average temperature/℃	土壤类型 Soil types	pH	林分类型 Stand types	林龄 Age of forest/ a
RP	23°35′20″N, 117°7′22″E	亚热带海洋性季风	1745.9	21.4	滨海潮沙土	5.90	纯林	8
WC	21°21′55″N, 110°43′23″E	亚热带季风	1597.8	22.5	滨海潮沙土	6.46	纯林	6
XW	20°28′7″N, 110°31′20″E	热带季风	1400	23.6	滨海潮沙土	5.16	纯林	7

分级标准 Grading standard	病级 Disease degree
树木长势极好,小枝浓绿,全株健康无病 The trees are in excellent condition, with dense green twigs, healthy and disease free throughout	0
树木长势良好,小枝枯梢 Trees in good condition, twigs with dead tips	1
树木长势一般,1/3小枝凋萎 Trees of average growth, 1/3 of twigs withered	2
树木长势较差,1/3至2/3小枝凋萎 Poor tree growth, 1/3 to 2/3 of twigs withered	3
树木长势差,2/3以上小枝凋萎 Poor tree growth, with more than 2/3 of twigs withered	4
病株枯死,小枝全部凋萎 The disease plant dead and all twigs withered	5

分级标准 Grading standard	病级 Disease degree
树木长势极好,小枝浓绿,全株健康无病 The trees are in excellent condition, with dense green twigs, healthy and disease free throughout	0
树木长势良好,小枝枯梢 Trees in good condition, twigs with dead tips	1
树木长势一般,1/3小枝凋萎 Trees of average growth, 1/3 of twigs withered	2
树木长势较差,1/3至2/3小枝凋萎 Poor tree growth, 1/3 to 2/3 of twigs withered	3
树木长势差,2/3以上小枝凋萎 Poor tree growth, with more than 2/3 of twigs withered	4
病株枯死,小枝全部凋萎 The disease plant dead and all twigs withered	5

病级 Disease degree		0	1	2	3	4		5
感病株数 Number of diseased plants	RP	49	0	0	1	0		0
		49	0	0	0	0		1
		49	0	1	0	0		0
	WC	47	0	0	1	0		2
		46	0	1	1	0		2
		47	0	0	1	0		2
	XW	45	0	1	0	0		4
		43	0	1	1	0		5
		44	0	1	0	0		5
感病指数 Disease index	RP		WC				XW
感病指数 Disease index	1.33±0.88c		5.47±0.67b				10.53±2.33a

Correlation Analysis of the Occurrence of Bacterial Wilt and Physicochemical Properties and Enzyme Activity of Root-Zone Soil of Casuarina spp.

木麻黄根区土壤理化特性及酶活性与青枯病发生关联分析

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土壤指标 Indicators of soil	地区 Regions	DRS	HRS
pH	RP	5.29±0.12a	5.50±0.06a
	WC	6.17±0.10 bB	6.70±0.11aA
	XW	5.04±0.07a	5.16±0.07a
EC/ (μS∙cm^-1)	RP	30.70±7.60a	33.30±7.61a
	WC	40.27±7.70a	30.00±6.23a
	XW	40.43±2.08a	32.47±5.61a
w_(SOM)/ (g∙kg^-1)	RP	3.07±0.30bB	4.37±0.36aA
	WC	5.44±0.14b	6.21±0.19a
	XW	2.41±0.18bB	3.63±0.15aA
w_(TN)/ (g∙kg^-1)	RP	0.17±0.01b	0.22±0.03a
	WC	0.21±0.02a	0.26±0.04a
	XW	0.14±0.01a	0.16±0.02a
w_(AN)/ (mg∙kg^-1)	RP	29.23±2.15a	33.69±0.98a
	WC	17.37±0.57bB	26.79±1.74aA
	XW	23.00±1.22bB	27.83±1.01aA
w_(TP)/ (g∙kg^-1)	RP	0.12±0.01bB	0.21±0.01aA
	WC	0.14±0.04a	0.21±0.02a
	XW	0.07±0.01a	0.07±0.01a
w_(AP)/ (mg∙kg^-1)	RP	12.01±1.97a	16.31±1.66a
	WC	5.73±1.63bB	18.23±1.05aA
	XW	1.02±0.15bB	1.86±0.08aA
w_(TK)/ (g∙kg^-1)	RP	8.90±0.02a	9.33±0.19a
	WC	5.00±0.37a	5.71±0.20a
	XW	2.55±0.61a	2.83±0.57a
w_(AK)/ (mg∙kg^-1)	RP	41.41±0.82bB	55.37±1.52aA
	WC	18.85±2.52bB	27.83±0.81aA
	XW	20.97±1.85bB	37.61±2.41aA

类别 Category	发病等级 Disease degree	pH	SOM	TN	TP	TK	EC	AP	AK	AN
发病等级 Disease degree	1.000
pH	-0.423	1.000
SOM	-0.264	0.762^*	1.000
TN	-0.474	0.611	0.883^**	1.000
TP	-0.503	0.571	0.653	0.527	1.000
TK	-0.949^**	0.318	0.250	0.517	0.485	1.000
EC	0.211	0.025	0.483	0.600	0.000	-0.050	1.000
AP	-0.896^**	0.561	0.350	0.500	0.644	0.800^**	-0.183	1.000
AK	-0.609	-0.374	-0.285	0.067	0.172	0.720^*	-0.084	0.477	1.000
AN	-0.476	-0.420	-0.527	-0.318	0.118	0.452	-0.460	0.385	0.739^*	1.000

土壤酶活性 Enzyme activity of soil	地区 Regions	DRS	HRS
URE/ (mg∙kg^-1∙h^-1)	RP	155.60±0.60a	161.08±4.09a
	WC	157.23±2.65a	163.54±0.98a
	XW	156.79±0.31b	165.25±0.86a
ACP/ (μg∙g^-1∙h^-1)	RP	36.60±4.83a	53.67±5.30a
	WC	10.56±0.18a	20.98±4.80a
	XW	24.49±4.53bB	66.73±11.94aA
INV/ (0.1 mg∙kg^-1∙h^-1)	RP	1.05±0.09a	0.77±0.13a
	WC	0.83±0.17a	0.46±0.20a
	XW	1.20±0.32b	0.49±0.10a
CAT/ (0.1 mg∙g^-1∙h^-1)	RP	0.65±0.01b	0.76±0.07a
	WC	0.71±0.01b	0.82±0.01a
	XW	0.56±0.02a	0.62±0.01a
POD/ (mg∙g^-1∙h^-1)	RP	0.80±0.01a	0.91±0.04a
	WC	0.39±0.04a	0.43±0.02a
	XW	0.44±0.02bB	0.75±0.15aA
PPO/ (mg∙g^-1∙h^-1)	RP	23.45±1.80bB	74.97±3.24aA
	WC	30.43±3.22bB	59.17±9.46aA
	XW	59.63±8.87b	79.70±4.30a

类别 Category	发病等级 Disease degree	土壤酶活性Enzyme activity
类别 Category	发病等级 Disease degree	URE	ACP	INV	CAT	POD		PPO
发病等级Disease degree	1.000
URE	0.316	1.000
ACP	-0.369	-0.200	1.000
INV	-0.053	0.133	-0.050	1.000
CAT	-0.476	-0.151	-0.485	-0.335	1.000
POD	-0.580	0.050	0.767^*	0.100	-0.243		1.000
PPO	0.896^**	-0.067	-0.200	-0.017	-0.519		-0.500		1.000

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