Distribution Pattern of Antibiotic Resistant Bacteria in Water Supply Reservoirs of Central China

doi:10.16258/j.cnki.1674-5906.2021.05.014

Ecology and Environment ›› 2021, Vol. 30 ›› Issue (5): 1017-1022.DOI: 10.16258/j.cnki.1674-5906.2021.05.014

• Research Articles • Previous Articles Next Articles

Distribution Pattern of Antibiotic Resistant Bacteria in Water Supply Reservoirs of Central China

ZHANG Kai¹^,²(), GUO Ziwei¹^,², WANG Qian¹^,², HAN Ya¹^,², LI Kuangjia³, ZHANG Zhongshuai¹^,²

1. School of Geographic Sciences, Xinyang Normal University, Xinyang 464000, China
2. Henan Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution/Xinyang Normal University, Xinyang 464000, China
3. Development Research Center, Ministry of Water Resources of People’s Republic of China, Beijing 100038, China

Received:2020-12-09 Online:2021-05-18 Published:2021-08-06

华中地区水库型水源地抗生素抗性细菌的赋存特征研究

张凯¹^,²(), 郭紫微¹^,², 王倩¹^,², 韩雅¹^,², 李贶家³, 张中帅¹^,²

1.信阳师范学院地理科学学院,河南信阳 464000
2.河南省水土污染协同防治重点实验室/信阳师范学院,河南信阳 464000
3.水利部发展研究中心,北京 100038

作者简介:张凯（1987年生）,男,讲师,博士,研究方向为环境微生物。E-mail:kaizhang1014@163.com
基金资助:
国家自然科学基金项目(42007209);国家自然科学基金项目(41807038);信阳师范学院"南湖学者奖励计划"青年项目

Abstract

Abstract:

Bacterial drug resistance caused by antibiotic abuse has aroused widespread concern. Water supply reservoirs, which are closely related to human health, have become a hot spot in antibiotic resistant bacteria researches (ARB). This study selected 11 water supply reservoirs to investigate ARB distribution pattern in water supply reservoirs of central China. The distribution pattern of five types of ARB was analyzed both in the waterbody and sediment of the reservoirs. The results showed that the abundances of tetracycline and sulfanilamide ARB were the highest in both the water and sediment samples, while the abundance of quinolone ARB were the lowest. Mental analysis showed significant positive correlation (P<0.05) between the ARB abundances in sediments and overlying waterbody. The mass exchange between sediments and waterbody might be an important factor leading to this result. The Spearman correlation analysis showed certain correlation among ARBs, and the co-selection of antibiotics might be the underlying reason. The conditional pathogens screened in this study were resistant to a variety of antibiotics, which might pose potential threats to human health. In addition, the lower total heterotrophic bacteria number in reservoirs and the higher bacteria removal efficiency in drinking water plants can ensure the quality of water supply in central China, although the results of this study show that the reservoir sediments are polluted by fecal sources to a certain extent.

Key words: central China, water supply reservoirs, antibiotic resistance bacteria, distribution pattern, surface water, sediment

摘要：

抗生素的滥用导致了细菌耐药性的变化,而水库型水源地抗生素抗性细菌（Antibiotic resistant bacteria,ARB）与人类健康密切相关。为明确华中地区水库型水源地中ARB的赋存特征,选取华中地区11个大型水库型水源地为研究对象,分析了5类ARB在水和沉积物中的分布规律。结果表明,华中地区水库型水源地水和沉积物中,丰度最高的ARB均以四环素类ARB及磺胺类ARB的丰度最高,喹诺酮类ARB在本研究最低。Mantel分析结果表明,沉积物和上覆水中ARB丰度呈显著性正相关（P<0.05）,沉积物和水中的物质交换可能是导致这一结果的重要因素。Spearman相关性分析结果表明ARB之间存在一定的相关性,抗生素的共选择作用可能是造成这一结果的潜在因素。研究中筛选的条件致病菌分别对多种抗生素均具有抗性,可能会对人体健康产生潜在威胁。此外,虽然该研究的结果表明水库沉积物在一定程度上受到粪源污染,但华中地区水库环境较低的总异养菌数和饮用水厂较高的细菌去除效率在一定程度上能够保证供水质量。

关键词: 华中地区, 水库型水源地, 抗生素抗性细菌, 赋存特征, 上覆水, 沉积物

CLC Number:

X52
X171.5

ZHANG Kai, GUO Ziwei, WANG Qian, HAN Ya, LI Kuangjia, ZHANG Zhongshuai. Distribution Pattern of Antibiotic Resistant Bacteria in Water Supply Reservoirs of Central China[J]. Ecology and Environment, 2021, 30(5): 1017-1022.

张凯, 郭紫微, 王倩, 韩雅, 李贶家, 张中帅. 华中地区水库型水源地抗生素抗性细菌的赋存特征研究[J]. 生态环境学报, 2021, 30(5): 1017-1022.

Figures/Tables 5

References 34

[1]	ALLEN H, DONATO J, WANG H, et al., 2010. Call of the wild: Antibiotic resistance genes in natural environments[J]. Nature Reviews Microbiology, 8(4): 251-259. DOI URL
[2]	BOYD L B, MAYNARD M J, MORGAN-LINNELL S K, et al., 2009. Relationships among ciprofloxacin, gatifloxacin, levofloxacin, and norfloxacin MICs for fluoroquinolone-resistant Escherichia coli clinical isolates[J]. Antimicrobial Agents and Chemotherapy, 53(1): 229-234. DOI URL
[3]	CHEN Y H, SU J Q, ZHANG J Y, et al., 2019. High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system[J]. Water Research, 149: 179-189. DOI URL
[4]	DANG C Y, XIA Y, ZHENG M S, et al., 2020. Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir[J]. Environment International, 136: 105449. DOI URL
[5]	GARCÍA J, GARCÍA-GALÁN M, DAY J, et al., 2020. A review of emerging organic contaminants (EOCs), antibiotic resistant bacteria (ARB), and antibiotic resistance genes (ARGs) in the environment: Increasing removal with wetlands and reducing environmental impacts[J]. Bioresource Technology, DOI:10.1016/j.biortech.2020.123228. DOI
[6]	GAO P, MUNIR M, IRENE X, 2012. Correlation of tetracycline and sulfonamide antibiotics with corresponding resistance genes and resistant bacteria in a conventional municipal wastewater treatment plant[J]. Science of Total Environment, 421-422: 173-183.
[7]	HAN Z M, AN W, YANG M, et al., 2020. Assessing the impact of source water on tap water bacterial communities in 46 drinking water supply systems in China[J]. Water Research, DOI:10.1016/j.watres.2020. 115469. DOI
[8]	HARNISZ M, KORZENIEWSKA M, GOLAS I, 2015. The impact of a freshwater fish farm on the community of river resistant bacteria and the structure of tetracycline resistance genes in water tetra cycline resistant bacteria and the structure of tetracycline resistance genes in river water[J]. Chemosphere, 128(1): 134-141. DOI URL
[9]	HARTHUG S, JUREEN R, MOHN S C, et al., 2002. The prevalence of faecal carriage of ampicillin-resistant and high-level gentamicin-resistant enterococci among inpatients at 10 major Norwegian hospitals[J]. Journal of Hospital Infection, 50(2): 145-154. DOI URL
[10]	JIANG H Y, ZHOU R J, YANG Y, et al., 2018. Characterizing the antibiotic resistance genes in a river catchment: Influence of anthropogenic activities[J]. Journal of Environmental Sciences, 69(7): 125-132. DOI URL
[11]	LIN M, LIANG J J, ZHANG X, et al., 2015. Genetic diversity of three classes of integrons in antibiotic-resistant bacteria isolated from Jiulong River in southern China[J]. Environmental Science and Pollution Research, 22(15): 11930-11939. DOI URL
[12]	LUO Y, WANG Q, LU Q, et al., 2014. An ionic liquid facilitates the proliferation of antibiotic resistance genes mediated by class I integrons[J]. Environmental Science & Technology Letters, 1(5): 266-270.
[13]	NNADOZIE C, ODUME O, 2019. Freshwater environments as reservoirs of antibiotic resistant bacteria and their role in the dissemination of antibiotic resistance genes[J]. Environmental Pollution, DOI:10.1016/j.envpol.2019.113067. DOI
[14]	PANG Y C, XI J Y, LI G Q, et al., 2015. Prevalence of antibiotic-resistant bacteria in a lake for the storage of reclaimed water before and after usage as cooling water[J]. Environmental Science: Processes & Impacts, 17(6): 1182-1189.
[15]	QIAO M, YING G G, SINGER A C, et al., 2018. Review of antibiotic resistance in China and its environment[J]. Environment International, 10(16): 160-172.
[16]	QIU J R, ZHAO T, LIU Q Y, et al., 2006. Residual veterinary antibiotics in pig excreta after oral administration of sulfonamides[J]. Environmental Geochemistry and Health, 38(2): 549-556. DOI URL
[17]	IWANE T, URASE T, YAMAMOTO K, 2001. Possible impact of treated wastewater discharge on incidence of antibiotic resistant bacteria in river water[J]. Water Science and Technology, 43(2): 91-99.
[18]	YANG Y, ZHOU R J, CHEN B W, et al., 2018. Characterization of airborne antibiotic resistance genes from typical bioaerosol emission sources in the urban environment using metagenomic approach[J]. Chemosphere, 213: 463-471. DOI URL
[19]	ZHANG K, NIU Z G, LV Z W, et al., 2017. Occurrence and distribution of antibiotic resistance genes in water supply reservoirs in Jingjinji area, China[J]. Ecotoxicology, 26(9): 1284-1292. DOI URL
[20]	ZHANG K, XIN R, ZHAO Z, et al., 2020. Antibiotic Resistance Genes in drinking water of China: Occurrence, distribution and influencing factors[J]. Ecotoxicology and Environmental Safety, DOI:10.1016/ j.ecoenv.2019.109837. DOI
[21]	ZHANG Q Q, YING G, PAN C G, et al., 2015. Comprehensive Evaluation of Antibiotics Emission and Fate in the River Basins of China: Source Analysis, Multimedia Modeling, and Linkage to Bacterial Resistance[J]. Environmental Science & Technology, 49(11): 6772-6782. DOI URL
[22]	姜春霞, 黎平, 李森楠, 等, 2019. 海南东寨港海水和沉积物中抗生素抗性基因污染特征研究[J]. 生态环境学报, 28(1): 132-139.
	JIANG C X, LIN P, LI S N, et al., 2019. Pollution characteristics of antibiotic resistance genes in seawater and sediment of Dongzhai Harbor, Hainan Province[J]. Ecology and Environmental Sciences, 28(1): 128-135.
[23]	廖杰, 魏晓琴, 肖燕琴, 等, 2020. 莲花水库水体中抗生素污染特征及生态风险评价[J]. 环境科学, 41(9): 4081-4087.
	LIAO J, WEI X Q, XIAO Y Q, et al., 2020. Pollution characteristics and risk assessment of antibiotics in Lianhua reservoir[J]. Environmental Science, 41(9): 4081-4087.
[24]	刘珊珊, 杨栋, 张坤明, 等, 2018. 天津市某地区自来水中抗生素耐药菌及其相关基因的调查[J]. 环境与健康杂志, 35(2): 166-169.
	LIU S S, YANG D, ZHANG K M, et al., 2018. Antibiotic resistance bacterias and related genes in tap water in an area in Tianjin[J]. Journal of Environment and Health, 35(2): 166-169.
[25]	陆桂华, 马倩, 2009. 太湖水域“湖泛”及其成因研究[J]. 水科学进展, 20(3): 130-134.
	LU G H, MA Q, 2009. Research on “Lake Flooding” of Taihu Lake and the related reasons[J]. Advances in Water Sciences, 20(3): 130-134.
[26]	孟磊, 杨兵, 薛南冬, 2015. 氟喹诺酮类抗生素环境行为及其生态毒理研究进展[J]. 生态毒理学报, 10(2): 76-88.
	MENG L, YANG B, XUE N D, 2015. A review on environmental behaviors and ecotoxicology of fluoroquinolone antibiotics[J]. Asian Journal of Ecotoxicology, 10(2): 76-88.
[27]	缪羽晨, 刘尧成, 2019. 我国华中地区区域经济发展差异比较研究[J]. 经济视角, 279(6): 73-79.
	MIAO Y C, LIU Y C, 2019. A comparative study on the differences of regional economic development in central China[J]. Economic Vision, 279(6): 73-79.
[28]	彭佳雯, 黄贤金, 钟太洋, 等, 2011. 中国经济增长与能源碳排放的脱钩研究[J]. 资源科学, 33(4): 34-41.
	PENG J W, HUANG X J, ZHONG T Y, et al., 2011. Research on the decoupling of China’s economic growth and energy carbon emissions[J]. Resource Science, 33(4): 34-41.
[29]	钱岩, 满江红, 王先良, 等, 2013. 山东主要河湖地表水微生物学指标调查[J]. 环境与健康杂志, 30(3): 241-243.
	QIAN Y, MAN J H, WANG X L, et al., 2013. Microbial pollution evaluation of main surface water in Shandong[J]. Journal of Environment and Health, 30(3): 241-243.
[30]	孙增灵, 范喜梅, 阮嘉玲, 等, 2011. 金银湖水质细菌学检测与分析[J]. 安徽农业科学, 39(34): 21238-21239.
	SUN Z L, FAN X M, RUAN J L, et al., 2011. Bacteriological detection and analysis of water quality of Jinyin Lake[J]. Journal of Anhui Agricultural Sciences, 39(34): 21238-21239.
[31]	叶繁, 冯时欢, 吴佳佳, 2019. 养殖虾塘常见耐药菌的分离鉴定与耐药基因检测[J]. 生态环境学报, 28(9): 135-141.
	YE F, FENG S H, WU J J, et al., 2019. Antibiotic resistant bacterial isolation and identification from shrimp ponds and their antibiotic resistance genes detection[J]. Ecology and Environmental Sciences, 28(9): 1843-1849.
[32]	伊丽丽, 焦文涛, 陈卫平, 2013. 不同抗生素在剖面土壤中的吸附特征[J]. 环境化学, 32(12): 2357-2363.
	YI L L, JIAO W T, CHEN W P, 2013. Adsorption characteristics of different antibiotics in soil profile[J]. Environmental Chemistry, 32(12): 2357-2363.
[33]	张皓清, 贾永刚, 王凯歌, 等, 2020. 水体沉积物中内源污染物释放研究进展[J]. 应用化工, 49(337): 228-232.
	ZHANG H Q, JIA Y G, WANG K G, et al., 2020. Research progress on release of endogenous pollutants in water sediments[J]. Applied Chemical Industry, 49(337): 228-232.
[34]	朱永官, 陈青林, 苏建强, 等, 2018. 环境中抗生素与抗性基因组的研究[J]. 科学观察, 12(6): 60-63.
	ZHU Y G, CHEN Q L, SU J Q, et al., 2018. Research on antibiotics and antibiotic resisomes in the environment[J]. Science Focus, 12(6): 60-63.

抗生素类别 Category	名称 Name	ρ/(mg∙L^-1)	参考文献 References
磺胺类 Sulfonamides	磺胺甲恶唑 Sulfamethoxazole	50	Gao et al., 2012
β-内酰胺类 β-lactem	氨苄西林 Ampicillin	32	Harthug et al., 2002
四环素类 Tetracycline	四环素 Tetracycline	16	Harnisz et al., 2015
喹诺酮类 Quinolone	诺氟沙星 Norfloxacin	10	Lin et al., 2015
氨基糖苷类 Aminoglycosides	链霉素 Streptomycin	30	Luo et al., 2014

抗生素类别 Category	名称 Name	ρ/(mg∙L^-1)	参考文献 References
磺胺类 Sulfonamides	磺胺甲恶唑 Sulfamethoxazole	50	Gao et al., 2012
β-内酰胺类 β-lactem	氨苄西林 Ampicillin	32	Harthug et al., 2002
四环素类 Tetracycline	四环素 Tetracycline	16	Harnisz et al., 2015
喹诺酮类 Quinolone	诺氟沙星 Norfloxacin	10	Lin et al., 2015
氨基糖苷类 Aminoglycosides	链霉素 Streptomycin	30	Luo et al., 2014

水库 Reservoir	水样 Water/(CFUs∙mL^-1)	沉积物 Sediment/(CFUs∙g^-1)
HN1	9.00×10³	8.33×10⁴
HN2	4.25×10³	3.67×10⁵
HN3	1.88×10³	8.33×10⁵
HN4	5.93×10³	1.34×10⁵
HB1	3.37×10³	9.33×10⁴
HB2	3.95×10³	1.10×10⁵
HB3	2.75×10³	9.33×10⁴
HU1	2.50×10³	1.28×10⁵
HU2	1.18×10³	1.18×10⁵
HU3	3.50×10³	3.57×10⁴
HU4	4.87×10³	7.00×10⁵

水库 Reservoir	水样 Water/(CFUs∙mL^-1)	沉积物 Sediment/(CFUs∙g^-1)
HN1	9.00×10³	8.33×10⁴
HN2	4.25×10³	3.67×10⁵
HN3	1.88×10³	8.33×10⁵
HN4	5.93×10³	1.34×10⁵
HB1	3.37×10³	9.33×10⁴
HB2	3.95×10³	1.10×10⁵
HB3	2.75×10³	9.33×10⁴
HU1	2.50×10³	1.28×10⁵
HU2	1.18×10³	1.18×10⁵
HU3	3.50×10³	3.57×10⁴
HU4	4.87×10³	7.00×10⁵

Distribution Pattern of Antibiotic Resistant Bacteria in Water Supply Reservoirs of Central China

华中地区水库型水源地抗生素抗性细菌的赋存特征研究

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