纳米银对玉米幼苗傅里叶红外光谱特性的影响

doi:10.16258/j.cnki.1674-5906.2023.07.011

生态环境学报 ›› 2023, Vol. 32 ›› Issue (7): 1285-1292.DOI: 10.16258/j.cnki.1674-5906.2023.07.011

纳米银对玉米幼苗傅里叶红外光谱特性的影响

赵海英¹(), 刘致远², 袁梦仙², 张卿雯², 张琼², 曹际玲²^,^*()

1.通化师范学院历史与地理学院，吉林通化 134001
2.江西农业大学国土资源与环境学院/江西省鄱阳湖流域农业资源与生态重点实验室，江西南昌 330045

收稿日期:2022-12-15 出版日期:2023-07-18 发布日期:2023-09-27
通讯作者: * 曹际玲。E-mail: jlcao2008@163.com
作者简介:赵海英（1979年生），女，讲师，博士，主要从事污染评价与修复方面的研究工作。E-mail: hyzgeography@163.com
基金资助:
吉林省科技发展计划项目(YDZJ202201ZYTS512);国家自然科学基金项目(31800520)

Effects of Silver Nanoparticles on FTIR Spectroscopic Characterization of Maize Seedlings

ZHAO Haiying¹(), LIU Zhiyuan², YUAN Mengxian², ZHANG Qingwen², ZHANG Qiong², CAO Jiling²^,^*()

1. School of History and Geography, Tonghua Normal University, Tonghua 134001, P. R. China
2. School of Land Resources and Environment, Jiangxi Agricultural University/Innovative Integration Center of Industry and Education in Resource Utilization of Agricultural Waste, Prevention and Control of Non-point Pollution of Jiangxi Province, Nanchang 330045, P. R. China

Received:2022-12-15 Online:2023-07-18 Published:2023-09-27

摘要/Abstract

摘要：

随着纳米科技的飞速发展和纳米产品的广泛使用，纳米颗粒不可避免地进入土壤环境成为一种潜在环境污染物，对土壤生态系统产生潜在威胁。傅里叶变换红外光谱是一种基于有机物分子中极性键振动简便快速和高分辨率分析物质组成和含量的技术。为明确纳米颗粒对植物生长和主要代谢物的影响，以纳米银为供试材料，以玉米（Zea may）为供试植物，基于盆栽试验探究不同浓度纳米银（0、1.0、5.0、10.0 mg·kg^-1）的植物生物效应，分析了不同浓度纳米银对玉米幼苗生物量的影响，利用傅里叶红外光谱法研究了不同浓度纳米银对玉米幼苗根系和叶片有机物质的影响。研究结果表明，随着纳米银浓度增加玉米幼苗地上部和地下部生物量均呈降低趋势，高浓度纳米银（10.0 mg·kg^-1）显著降低了玉米幼苗地上部和地下部生物量，降幅分别达16.5%和22.2%。不同浓度纳米银对玉米幼苗根系和叶片有机物质产生了不同程度的影响。低浓度纳米银（1.0、5.0 mg·kg^-1）提高了玉米幼苗根系羧基、羟基等基团和有机酸、蛋白质等有机物质含量，有助于吸附和固定银离子，降低纳米银对玉米幼苗生长的影响。高浓度纳米银（10.0 mg·kg^-1）降低了玉米幼苗根系和叶片4000-400 cm^-1波数范围内官能团相关有机物质含量。由此可见，低浓度纳米银下植物可通过调节羟基、羧基等基团相关有机物质含量的变化抵抗纳米银对植物的影响，但高浓度纳米银不利于植物有机物质的合成，进而影响植物生长。该结果可为揭示纳米银对玉米幼苗生长和生理代谢的影响机理提供理论依据。

关键词: 纳米银, 玉米, 生物量, 傅里叶变换红外光谱, 物质成分和结构

Abstract:

With the rapid development of nanotechnology and the extensive usage of nanoproducts, it is inevitable for nanoparticles to enter into the soil environment and become a potential environmental contaminant, which poses a threat to soil ecosystems. In order to study the effects of nanoparticles on the growth and the main metabolites of plants, silver nanoparticles (AgNPs) were used as the test material, maize (Zea may L.) was used as the experimental plant, the effects of different levels of AgNPs (0 mg·kg^-1, 1.0 mg·kg^-1, 5.0 mg·kg^-1, and 10.0 mg·kg^-1) on the biomasses and the spectral characteristics of maize roots and leaves with Fourier transform infrared spectroscopy were investigated based on a pot experiment. Results showed that AgNPs at high concentration (10.0 mg·kg^-1) significantly decreased the aboveground and underground biomasses of maize seedlings by 16.5% and 22.2%, respectively. Different concentrations of AgNPs exerted distinct influences on the organic matter in the roots and leaves of maize seedlings. AgNPs at low concentrations (1.0 mg·kg^-1, 5.0 mg·kg^-1) increased the contents of hydroxyl and carboxyl groups, organic acids, and proteins in the maize roots, which might combine with Ag ions and thus reduce the stress of AgNPs on maize. AgNPs at high concentrations (10.0 mg·kg^-1) decreased organic matter contents related to functional groups at 4000-400 cm^-1 in the maize roots and leaves. Therefore, a low concentration of AgNPs may induce plants to resist AgNPs by adjusting the contents of hydroxyl and carboxyl groups, organic acids, and proteins. AgNPs at high concentrations may inhibit the synthesis of organic matter and thus inhibit plant growth. These results may provide a theoretical basis for revealing the mechanism of the effects of AgNPs on the growth and physiological metabolism of maize seedlings.

Key words: Silver nanoparticles, Maize, Biomass, Fourier transform infrared spectroscopy, Material composition and structure

中图分类号:

X171.5

赵海英, 刘致远, 袁梦仙, 张卿雯, 张琼, 曹际玲. 纳米银对玉米幼苗傅里叶红外光谱特性的影响[J]. 生态环境学报, 2023, 32(7): 1285-1292.

ZHAO Haiying, LIU Zhiyuan, YUAN Mengxian, ZHANG Qingwen, ZHANG Qiong, CAO Jiling. Effects of Silver Nanoparticles on FTIR Spectroscopic Characterization of Maize Seedlings[J]. Ecology and Environment, 2023, 32(7): 1285-1292.

图/表 8

图1 不同浓度纳米银处理下玉米幼苗地上部和地下部生物量不同小写字母表示处理间差异显著（P<0.05）

Figure 1 Shoot dry biomass and root dry biomass of maize seedlings under different concentrations of AgNPs

表1 红外光谱中有机物、官能团及其对应的FTIR特征波长

Table 1 Main organic matter, functional groups and their corresponding FTIR characteristic wavelengths

波数/ cm^-1	主要基团	有机物
3405	O-H和N-H的伸缩振动	蛋白质和碳水化合物
2925	-CH₂的反对称伸缩振动	脂类和蛋白质
2850	-CH₂的对称伸缩振动	脂类和蛋白质
1729	C=O的伸缩振动	细胞壁果胶成分中的脂类化合物
1637	C=O的伸缩振动	蛋白质酰胺I带
1565	N-H的弯曲振动	蛋白质酰胺II带
1384	C-H弯曲振动	膜和胞壁含油脂化合物
1250	C=O的伸缩振动或NH₂变形	蛋白质酰胺III带
1158	C-O-C的伸缩振动	蛋白质分子氨基酸残基、纤维素糖苷
1110	C-O的伸缩振动	碳水化合物
1060	C-O的伸缩振动	碳水化合物
1050	C-C和C-O的伸缩振动	碳水化合物

图2 不同浓度纳米银处理下玉米幼苗根系的傅里叶红外光谱特征（4000-400 cm-1）

Figure 2 FTIR spectra of the roots of maize seedlings under different concentration of AgNPs (4000-400 cm-1)

表2 不同浓度纳米银处理下玉米根系特征吸收峰波数

Table 2 Characteristic peak wave number of FTIR in maize root under different concentration of AgNPs

处理	波数/cm^-1
处理	3405	2925	1731	1647	1637	1603	1559	1516	1490	1417	1384	1256	1201	1159	1039
L	3396	2922	1731	1647	1637	1604	1559	1516	1489	1417	1384	1255	1201	1159	1039
M	3400	2922	1731	1647	1633	1604	1559	1516	1490	1417	1384	1254	1201	1157	1037
H	3406	2924	1731	1647	1637	1604	1559	1516	1490	1417	1384	1256	1201	1156	1037

图3 不同浓度纳米银处理下玉米幼苗根系的傅里叶红外光谱图（1800-800 cm-1）

Figure 3 FTIR spectra of the roots of maize seedlings under different concentration of AgNPs (1800-800 cm-1)

图4 不同浓度纳米银处理下玉米幼苗叶片的傅里叶红外光谱图（4000-400 cm-1）

Figure 4 FTIR spectra of the leaves of maize seedlings under different concentration of AgNPs (4000-400 cm-1)

图5 不同浓度纳米银处理下玉米幼苗叶片的傅里叶红外光谱图（1800-800 cm-1）

Figure 5 FTIR spectra of the leaves of maize seedlings under different concentration of AgNPs (1800-800 cm-1)

表3 不同浓度纳米银处理下玉米叶片特征吸收峰波数

Table 3 Characteristic peak wave number of FTIR in maize leaves under different concentration of AgNPs

处理	波数/cm^-1
处理	3405	2918	2850	1731	1633	1559	1518	1490	1384	1320	1258	1202	1160	1104	1056	1038
L	3415	2918	2850	1731	1633	-	-	-	1384	1320	1257	1203	1160	1104	1057	1038
M	3406	2918	2850	1731	1633	1559	1517	1490	1384	1320	1256	1203	1160	1105	1056	1038
H	3407	2919	2850	1731	1647	1559	-	1490	1384	1320	1257	1203	1160	1104	1057	1038

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纳米银对玉米幼苗傅里叶红外光谱特性的影响

Effects of Silver Nanoparticles on FTIR Spectroscopic Characterization of Maize Seedlings

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