生态环境学报 ›› 2026, Vol. 35 ›› Issue (8): 1267-1275.DOI: 10.16258/j.cnki.1674-5906.2026.08.010
贺立琨(
), 王宇东, 刘汝海, 王云旭, 王艳(
), 郑浩, 李锋民
收稿日期:2025-04-14
修回日期:2026-06-28
接受日期:2026-07-29
出版日期:2026-08-18
发布日期:2026-08-17
通讯作者:
E-mail: 作者简介:贺立琨(2000年生),男,硕士研究生,研究方向为土壤修复。E-mail: helikun619@163.com
基金资助:
He Likun(
), Wang Yudong, Liu Ruhai, Wang Yunxu, Wang Yan(
), Zheng Hao, Li Fengmin
Received:2025-04-14
Revised:2026-06-28
Accepted:2026-07-29
Online:2026-08-18
Published:2026-08-17
摘要:
【目的】铁是作物必需的微量元素,铁的氧化物影响土壤团聚体的形成和理化性质,本研究旨在通过田间试验验证生物炭和铁基材料的田间联合施用能否发挥对酸化花生田土壤的协同改良效应。【方法】在酸化花生田开展生物炭与铁基材料联合施用(BC+Fe0;BC+Fe3O4)田间试验,分析了联合施用对土壤理化性质、微生物群落特征以及花生产量的影响,探究联合施用对土壤改良的潜力。【结果】研究发现,联合施用显著提高了土壤pH值(从初始的5.48-5.83增至5.93-6.37)、TOC含量(从8.47-9.11 g·kg−1提升至11.35-13.55 g·kg−1),同时大粒径(>0.25 mm)团聚体比例增加,土壤容重降低。铁基材料单独施用增加了土壤中游离态、络合态氧化铁与二价铁离子的含量(4.56%-35.09%)。联合施用后土壤中游离态、无定型氧化铁和二价铁离子的含量增加;土壤Shannon和Chao1指数较对照组提高,3种优势菌门增加0.75%-1.59%。BC和Fe3O4联合施用组的单位产量、百果质量最大,这与土壤性质的改变、可利用性铁等营养物质的增加有关。【结论】生物炭与铁基材料的联合施用是一种有效的土壤改良策略,它能够提高土壤质量,增加微生物多样性,并增强花生的生长表现。该研究为花生田土壤改良提供了新的策略和科学依据,并有助于指导生物炭与铁基材料联合施用在农田土壤改良中的实际应用。
中图分类号:
贺立琨, 王宇东, 刘汝海, 王云旭, 王艳, 郑浩, 李锋民. 生物炭和铁基材料联合施用对花生田土壤改良效果研究[J]. 生态环境学报, 2026, 35(8): 1267-1275.
He Likun, Wang Yudong, Liu Ruhai, Wang Yunxu, Wang Yan, Zheng Hao, Li Fengmin. Effect of the Combined Application of Biochar and Iron-Based Materials on Soil Improvement in Peanut Fields[J]. Ecology and Environmental Sciences, 2026, 35(8): 1267-1275.
图1 花生田各处理组根际土与非根际土pH、TOC含量、总氮、电导率 n=9,下同
Figure 1 pH, total organic carbon (TOC) content, total nitrogen, and electrical conductivity of rhizosphere and bulk soils in each treatment group of the peanut field
| 土壤理化性质及产量 | pH | 电导率 | TN | TOC | 游离铁 | 无定型铁 | 络合态铁 | Fe2+ | 花生单位面积产量 |
|---|---|---|---|---|---|---|---|---|---|
| pH | 1 | ||||||||
| 电导率 | −0.24 | 1 | |||||||
| TN | 0.35 | −0.42 | 1 | ||||||
| TOC | 0.71*** | −0.45 | 0.76*** | 1 | |||||
| 游离铁 | 0.53* | −0.19 | 0.18 | 0.33 | 1 | ||||
| 无定型铁 | 0.74*** | −0.52* | 0.49* | 0.82*** | 0.45 | 1 | |||
| 络合态铁 | 0.33 | −0.48* | 0.37 | 0.52* | 0.13 | 0.59** | 1 | ||
| Fe2+ | 0.75*** | −0.41 | 0.51* | 0.60** | 0.57* | 0.62** | 0.54* | 1 | |
| 花生单位面积产量 | 0.61** | −0.036 | 0.041 | 0.37 | 0.21 | 0.34 | 0.15 | 0.35 | 1 |
表1 根际土壤理化性质及产量相关性分析
Table 1 Correlation analysis of physicochemical properties of rhizosphere soil
| 土壤理化性质及产量 | pH | 电导率 | TN | TOC | 游离铁 | 无定型铁 | 络合态铁 | Fe2+ | 花生单位面积产量 |
|---|---|---|---|---|---|---|---|---|---|
| pH | 1 | ||||||||
| 电导率 | −0.24 | 1 | |||||||
| TN | 0.35 | −0.42 | 1 | ||||||
| TOC | 0.71*** | −0.45 | 0.76*** | 1 | |||||
| 游离铁 | 0.53* | −0.19 | 0.18 | 0.33 | 1 | ||||
| 无定型铁 | 0.74*** | −0.52* | 0.49* | 0.82*** | 0.45 | 1 | |||
| 络合态铁 | 0.33 | −0.48* | 0.37 | 0.52* | 0.13 | 0.59** | 1 | ||
| Fe2+ | 0.75*** | −0.41 | 0.51* | 0.60** | 0.57* | 0.62** | 0.54* | 1 | |
| 花生单位面积产量 | 0.61** | −0.036 | 0.041 | 0.37 | 0.21 | 0.34 | 0.15 | 0.35 | 1 |
图4 花生田各处理土壤微生物相对丰度以及相互关系 变形菌门包括Alphaproteobacteria、Gammaproteobacteria,厚壁菌门包括Actinobacteria、Bacilli、Blastocatellia,酸杆菌门包括Acidobacteriae、Thermoleophilia
Figure 4 Relative abundance of soil microorganisms in each treatment of the peanut field, and their interrelationships
| 处理组 | 单位产量/ (kg·hm-2) | 百果质量/ g | 双仁果占比/% | 每公顷植株 鲜质量/kg |
|---|---|---|---|---|
| CK | 8176.35±195.60 | 231.96±5.36 | 82±3.78 | 21421.05±1260 |
| Fe0 | 8199.60±184.05 | 234.07±5.31 | 86 ±3.05 | 21361.05±1620.9 |
| Fe3O4 | 8168.85±174.60 | 236.77±12.48 | 82 ±2.08 | 21481.05±1377.75 |
| BC | 8479.35±375.90 | 235.57±14.47 | 83 ±6.92 | 21691.05±2967.45 |
| BC+ Fe0 | 8427.75±309.75 | 240.43±8.34 | 84 ±4.16 | 21661.05±453 |
| BC+Fe3O4 | 8500.35±429.75 | 252.03±11.21 | 89 ±1.73 | 21631.05±2349.3 |
表2 花生田各处理组花生指标
Table 2 Peanut indices in different treatment groups of peanut fields
| 处理组 | 单位产量/ (kg·hm-2) | 百果质量/ g | 双仁果占比/% | 每公顷植株 鲜质量/kg |
|---|---|---|---|---|
| CK | 8176.35±195.60 | 231.96±5.36 | 82±3.78 | 21421.05±1260 |
| Fe0 | 8199.60±184.05 | 234.07±5.31 | 86 ±3.05 | 21361.05±1620.9 |
| Fe3O4 | 8168.85±174.60 | 236.77±12.48 | 82 ±2.08 | 21481.05±1377.75 |
| BC | 8479.35±375.90 | 235.57±14.47 | 83 ±6.92 | 21691.05±2967.45 |
| BC+ Fe0 | 8427.75±309.75 | 240.43±8.34 | 84 ±4.16 | 21661.05±453 |
| BC+Fe3O4 | 8500.35±429.75 | 252.03±11.21 | 89 ±1.73 | 21631.05±2349.3 |
| [1] |
Bolan S, Sharma S, Mukherjee S, et al., 2024. Biochar modulating soil biological health: A review[J]. Science of The Total Environment, 914: 169585.
DOI URL |
| [2] |
Coomes O T, Miltner B C, 2017. Indigenous charcoal and biochar production: potential for soil improvement under shifting cultivation systems[J]. Land Degradation & Development, 28: 811-821.
DOI URL |
| [3] |
De Souza Junior J C, Monteiro F A, Xin X, et al., 2022. Nitrate supply affects copper nanoparticle accumulation by maize plant and availability of nutrients in rhizosphere and bulk soil[J]. Journal of Soil Science and Plant Nutrition, 22: 3598-3610.
DOI |
| [4] |
Fierer N, Jackson R B, 2006. The diversity and biogeography of soil bacterial communities[J]. Proceedings of the National Academy of Sciences, 103: 626-631.
DOI URL |
| [5] |
Geng N, Kang X, Yan X, et al., 2022. Biochar mitigation of soil acidification and carbon sequestration is influenced by materials and temperature[J]. Ecotoxicology and Environmental Safety, 232: 113241.
DOI URL |
| [6] |
Han L, Sun K, Yang Y, et al., 2020. Biochar’s stability and effect on the content, composition and turnover of soil organic carbon[J]. Geoderma, 364: 114184.
DOI URL |
| [7] |
He X L, Yang Y R, Huang B S, et al., 2024. An overview of characteristic factors of biochar as a soil improvement tool in rice growth- A review[J]. Environmental Research, 242: 117794.
DOI URL |
| [8] |
Kang M W, Yibeltal M, Kim Y H, et al., 2022. Enhancement of soil physical properties and soil water retention with biochar-based soil amendments[J]. Science of The Total Environment, 836: 155746.
DOI URL |
| [9] | Li S M, Tasnady D, 2023. Biochar for soil carbon sequestration: current knowledge, mechanisms, and future perspectives[J]. Journal of Carbon Research, 9(3): 67. |
| [10] |
Li T, Zhang H C, Wang X Y, et al., 2019. Soil erosion affects variations of soil organic carbon and soil respiration along a slope in Northeast China[J]. Ecological Processes, 8: 28.
DOI |
| [11] |
Liu D D, Ju W L, Jin X L, et al., 2021. Associated soil aggregate nutrients and controlling factors on aggregate stability in semiarid grassland under different grazing prohibition timeframes[J]. Science of The Total Environment, 777: 146104.
DOI URL |
| [12] |
Liu Z W, Zhu M T, Wang J M, et al., 2019. The responses of soil organic carbon mineralization and microbial communities to fresh and aged biochar soil amendments[J]. GCB Bioenergy, 11(12): 1408-1420.
DOI URL |
| [13] |
Murgia I, Marzorati F, Vigani G, et al., 2022. Plant iron nutrition: The long road from soil to seeds[J]. Journal of Experimental Botany, 73(6): 1809-1824.
DOI URL |
| [14] | Regelink I C, Voegelin A, Weng L P, et al., 2014. Characterization of Colloidal Fe from Soils Using Field-Flow Fractionation and Fe K-Edge X-ray Absorption Spectroscopy[J]. Environmental Science & Technology 48(8): 4307-4316. |
| [15] |
Rui M M, Ma C C, Hao Y, et al., 2016. Iron Oxide Nanoparticles as a Potential Iron Fertilizer for Peanut (Arachis hypogaea)[J]. Frontiers in Plant Science, 7: 815.
DOI PMID |
| [16] |
Sorrenti G, Masiello C A, Toselli M, 2016. Biochar interferes with kiwifruit Fe-nutrition in calcareous soil[J]. Geoderma, 272: 10-19.
DOI URL |
| [17] |
Sun J N, Yang R Y, Zhu J J, et al., 2019. Contrasting effects of corn straw biochar on soil water infiltration and retention at tilled and compacted bulk densities in the Yellow River Delta[J]. Canadian Journal of Soil Science, 99: 357-366.
DOI URL |
| [18] |
Sun Q, Meng J, Lan Y, et al., 2021. Long-term effects of biochar amendment on soil aggregate stability and biological binding agents in brown earth[J]. CATENA, 205: 105460.
DOI URL |
| [19] |
Tao E, Ji C, Cheng Y, et al., 2024. Effect of waste leather dander biochar on soil organic carbon sequestration[J]. Journal of Environmental Chemical Engineering, 12(3): 112633.
DOI URL |
| [20] |
Totsche K U, Amelung W, Gerzabek M H, et al., 2018. Microaggregates in soils[J]. Journal of Plant Nutrition and Soil Science, 181(1): 104-136.
DOI URL |
| [21] |
Wang T Q, Wang N Q, Lu Q F, et al., 2023. The active Fe chelator proline-2′-deoxymugineic acid enhances peanut yield by improving soil Fe availability and plant Fe status[J]. Plant, Cell & Environment, 46(1): 239-250.
DOI URL |
| [22] |
Wang X J, Jia Z K, Liang L Y, et al., 2018. Changes in soil characteristics and maize yield under straw returning system in dryland farming[J]. Field Crops Research, 218: 11-17.
DOI URL |
| [23] |
Widdel F, Schnell S, Heising S, et al., 1993. Ferrous iron oxidation by anoxygenic phototrophic bacteria[J]. Nature, 362: 834-836.
DOI |
| [24] |
Xu X, Pang D, Chen J, et al., 2018. Straw return accompany with low nitrogen moderately promoted deep root[J]. Field Crops Research, 221: 71-80.
DOI URL |
| [25] |
Zhang J, Shen J L, 2022. Effects of biochar on soil microbial diversity and community structure in clay soil[J]. Annals of Microbiology, 72: 35.
DOI |
| [26] |
Zhang P, Sheng G Y, Feng Y C, et al., 2005. Role of wheat-residue-derived char in the biodegradation of benzonitrile in soil: Nutritional stimulation versus adsorptive inhibition[J]. Environmental Science & Technology, 39(14): 5442-5448.
DOI URL |
| [27] |
Zhang S, Cui J W, Wu H, et al., 2021. Organic carbon, total nitrogen, and microbial community distributions within aggregates of calcareous soil treated with biochar[J]. Agriculture, Ecosystems & Environment, 314: 107408.
DOI URL |
| [28] |
Zheng H, Zhang Q, Liu G C, et al., 2019. Characteristics and mechanisms of chlorpyrifos and chlorpyrifos-methyl adsorption onto biochars: Influence of deashing and low molecular weight organic acid (LMWOA) aging and co-existence[J]. Science of The Total Environment, 657: 953-962.
DOI |
| [29] |
Zheng T Y, Hou J, Wu T, et al., 2024. Ferric oxide nanomaterials and plant-rhizobacteria symbionts cogenerate iron plaque for removing highly chlorinated contaminants in dryland soils[J]. Environmental Science & Technology, 58(25): 11063-11073.
DOI URL |
| [30] |
Zuo Y M, Zhang F S, 2011. Soil and crop management strategies to prevent iron deficiency in crops[J]. Plant Soil, 339: 83-95.
DOI URL |
| [31] | 高欣, 赵雪淞, 赵凤艳, 等, 2023. 有机培肥对连作花生土壤肥力及活性有机碳库的影响[J]. 土壤通报, 54(1): 67-76. |
| Gao X, Zhao X S, Zhao F Y, et al., 2023. Effects of organic fertilization on soil fertility and labile organic carbon pools under continuous peanut cropping[J]. Chinese Journal of Soil Science, 54(1): 67-76. | |
| [32] | 李霞, 周娅, 罗丽卉, 等, 2025. 油菜秸秆生物炭对成都平原土壤理化性质和水稻镉吸收的影响[J]. 西南农业学报, 38(1): 1-12. |
| Li X, Zhou Y, Luo L H, et al., 2025. Effects of rape-straw biochar on soil physicochemical properties and cadmium uptake by rice in the Chengdu Plain[J]. Southwest China Journal of Agricultural Sciences, 38(1): 1-12. | |
| [33] | 李雪菲, 靳拓, 张凯, 等, 2022. 微生物菌剂对设施辣椒秸秆原位堆肥土壤理化性质及细菌群落的影响[J]. 中国农业大学学报, 27(3): 33-43. |
| Li X F, Jin T, Zhang K, et al., 2022. Effects of microbial inoculants on soil physicochemical properties and bacterial communities during in-situ composting of pepper straw in protected cultivation[J]. Journal of China Agricultural University, 27(3): 33-43. | |
| [34] | 刘永秀, 左元梅, 1999. 玉米-花生混作对改善花生铁营养及固氮的影响[J]. 土壤通报, 30(2): 8-9, 12. |
| Liu Y X, Zuo Y M, 1999. Effects of maize-peanut intercropping on iron nutrition and nitrogen fixation of peanut[J]. Chinese Journal of Soil Science, 30(2): 8-9, 12. | |
| [35] | 沈云亭, 索炎炎, 张翔, 等, 2019. 河南省花生主产区土壤养分状况评价及施肥改进建议[J]. 河南农业科学, 48(9): 67-73. |
| Shen Y T, Suo Y Y, Zhang X, et al., 2019. Evaluation of soil nutrient status and fertilization improvement recommendations in the main peanut-producing areas of Henan Province[J]. Journal of Henan Agricultural Sciences, 48(9): 67-73. | |
| [36] | 宋旭昕, 刘同旭, 2021. 土壤铁矿物形态转化影响有机碳固定研究进展[J]. 生态学报, 41(20): 7928-7938. |
| Song X X, Liu T X, 2021. Research progress on the influence of iron mineral transformation on organic carbon sequestration in soils[J]. Acta Ecologica Sinica, 41(20): 7928-7938. | |
| [37] | 王瑞昕, 杨静, 方正, 等, 2021. 水分管理对水稻籽粒硒积累及根际土壤细菌群落多样性的影响[J]. 土壤学报, 58(6): 1574-1584. |
| Wang R X, Yang J, Fang Z, et al., 2021. Effects of water management on selenium accumulation in rice grains and rhizosphere soil bacterial community diversity[J]. Acta Pedologica Sinica, 58(6): 1574-1584. | |
| [38] | 杨继芬, 李永梅, 李春培, 等, 2023. 不同种植模式对坡耕地红壤团聚体中酶活性及养分含量的影响[J]. 土壤, 55(4): 787-794. |
| Yang J F, Li Y M, Li C P, et al., 2023. Effects of different cropping patterns on enzyme activities and nutrient contents in red-soil aggregates of sloping farmland[J]. Soils, 55(4): 787-794. | |
| [39] | 杨同荣, 崔贤, 徐婷, 等, 2021. 花生田土壤酸化改良集成技术研究与应用[J]. 农业与技术, 41(22): 70-72. |
| Yang T R, Cui X, Xu T, et al., 2021. Integrated technology for ameliorating soil acidification in peanut fields: Research and application[J]. Agriculture and Technology, 41(22): 70-72. | |
| [40] | 赵桂茹, 安曈昕, 欧阳铖人, 等, 2021. 青贮玉米氮投入对坡耕地土壤侵蚀及水稳性团聚体的影响[J]. 水土保持学报, 35(5): 72-79. |
| Zhao G R, An T X, Ouyang C R, et al., 2021. Effects of nitrogen input in silage maize on soil erosion and water-stable aggregates on sloping cropland[J]. Journal of Soil and Water Conservation, 35(5): 72-79. |
| [1] | 吴宇晴, 郭洁, 吴嘉慧, 刘谞承, 赵建刚. 土壤改良剂与植物复合对离子型稀土矿Pb污染的修复效果研究[J]. 生态环境学报, 2026, 35(3): 469-477. |
| [2] | 陈岩, 石成龙, 李璞君, 肖江, 陈光才. 含重金属林木生物质与骨粉共水热液相产物:解析及应用潜力初步评价[J]. 生态环境学报, 2025, 34(4): 642-652. |
| [3] | 卢聪. 生物炭负载纳米零价铁对沉积物中十溴二苯乙烷去除效果及机制[J]. 生态环境学报, 2024, 33(8): 1279-1288. |
| [4] | 赵乐依, 朱雪强, 刘健, 路平. 碳球负载纳米零价铁活化过硫酸盐降解水中恩诺沙星的性能研究[J]. 生态环境学报, 2024, 33(5): 757-770. |
| [5] | 丛鑫, 曹平, 王晓博. 生物炭负载纳米铁活化过硫酸盐去除土壤中的五氯联苯[J]. 生态环境学报, 2024, 33(2): 282-290. |
| [6] | 李璞君, 唐丽, 赵博, 邸东柳, 陈岩, 肖江, 陈光才. 生物炭基土壤改良剂对锑矿区土壤质量及亮叶桦生长的影响[J]. 生态环境学报, 2024, 33(12): 1953-1963. |
| [7] | 李晓晖, 艾仙斌, 李亮, 王玺洋, 辛在军, 孙小艳. 新型改性稻壳生物炭材料对镉污染土壤钝化效果的研究[J]. 生态环境学报, 2022, 31(9): 1901-1908. |
| [8] | 张义, 朱吉颖, 张聪, 王柔, 邹羿菱云, 吴振斌. 硅藻土在环境领域的研究和应用[J]. 生态环境学报, 2022, 31(12): 2441-2448. |
| [9] | 丛鑫, 李瑶, 王宇, 郑力. 生物炭基针铁矿复合材料对水中莠去津吸附特性研究[J]. 生态环境学报, 2021, 30(10): 2067-2075. |
| 阅读次数 | ||||||
|
全文 |
|
|||||
|
摘要 |
|
|||||