Earthworms are known to play an important role in improving soil, decomposing agricultural litter, and enhancing soil fertility and crop yield. The acute toxicity of 17 herbicides to Eisenia fetida was determined by a contact filter paper toxicity bioassay and an artificial soil toxicity bioassay. Results of 48 h by the contact filter paper toxicity bioassay, metamifop was high toxicity to E. fetida with LC50 (median lethal concentration) value of 7.6 μg∙cm-2; pretilachlor, haloxyfop-R-methyl, and bentazone were moderate toxicity to E. fetida with LC50 values of 10.7, 12.7, and 61.3 μg∙cm-2, respectively; quinclorac, MCPA-Na, topramezone, glufosinate ammonium, nicosulfuron, and bispyribac-sodium were low toxicity to E. fetida with LC50 values of 143.0, 198.2, 211.1, 466.9, 433.7, and 649.2 μg∙cm-2, respectively; pyrazosulfuron-ethyl, penoxsulam, pyribenzoxim, cyhalofop-butyl, fluoroglycofen, pendimethalin, and mesotrione were slight toxicity to E. fetida with LC50 values greater than 1000 μg∙cm-2. Results of 14 d by the artificial soil toxicity bioassay, haloxyfop-R-methyl showed the highest toxicity to E. fetida with LC50 value of 148.9 mg∙kg-1; followed by pretilachlor, MCPA-Na, pendimethalin, and metamifop, the LC50 values were 211.5, 335.0, 342.4, and 345.7 mg∙kg-1, respectively; the other tested herbicides were low toxicity to E. fetida with LC50 values greater than 500 mg∙kg-1. According to the guidelines of environmental safety evaluation for chemical pesticides, the 17 herbicides determined by an artificial soil toxicity bioassay were low toxicity to E. fetida. The results of this study can provide new data information for assessing the ecological risk of herbicides to earthworm, and also provide technical guidance for the safe use of herbicides in agricultural production.