Effect of Monoculture and Mixed Plantation with Coniferous and Broadleaved Tree Species on Soil Microbial Carbon Cycle Functional Gene Abundance

doi:10.16258/j.cnki.1674-5906.2023.10.001

Ecology and Environment ›› 2023, Vol. 32 ›› Issue (10): 1719-1731.DOI: 10.16258/j.cnki.1674-5906.2023.10.001

• Research Articles • Next Articles

Effect of Monoculture and Mixed Plantation with Coniferous and Broadleaved Tree Species on Soil Microbial Carbon Cycle Functional Gene Abundance

QIN Jiaqi¹(), XIAO Zhirou¹, MING Angang²^,³, ZHU Hao¹, TENG Jinqian¹, LIANG Zeli¹, TAO Yi²^,⁴, QIN Lin¹^,^*()

1. Guangxi Key Laboratory of Forest Ecology and Conservation/College of Forestry, Guangxi University, Nanning 530004, P. R. China
2. Experiment Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, P. R. China
3. Guangxi Youyiguan Forest Ecosystem Research Station, Pingxiang 532600, P. R. China
4. Youyiguan Forest Ecosystem Observation and Research Station of Guangxi, Pingxiang 532600, P. R. China

Received:2023-08-23 Online:2023-10-18 Published:2024-01-16
Contact: QIN Lin

针阔人工混交林及其纯林对土壤微生物碳循环功能基因丰度的影响

秦佳琪¹(), 肖指柔¹, 明安刚²^,³, 朱豪¹, 滕金倩¹, 梁泽丽¹, 陶怡²^,⁴, 覃林¹^,^*()

1.广西大学林学院/广西森林生态与保育重点实验室，广西南宁 530004
2.中国林业科学研究院热带林业实验中心，广西凭祥 532600
3.广西友谊关森林生态系统定位观测研究站，广西凭祥 532600
4.崇左凭祥友谊关森林生态系统广西野外科学观测站，广西凭祥 532600

通讯作者: 覃林
作者简介:秦佳琪（1998年生），女（壮族），硕士研究生，主要研究方向为森林生态学。E-mail: 18176721617@163.com
基金资助:
国家自然科学基金项目(31560109);国家自然科学基金项目(32071764);广西自然科学基金项目(2020GXNSFAA297208)

Abstract

Abstract:

Exploring the abundance of carbon cycle functional genes in soil microorganisms is important for understanding the mechanism of soil carbon cycling. However, the response characteristics of soil microbial carbon cycle functional gene abundance to different plantation types are still unclear. Based on the metagenomic sequencing data, soil physicochemical properties, and organic carbon components of soil samples from different soil layers (0-20, 20-40, 40-60 cm) under the mixed species plantation with Pinus massoniana and Erythrophleum fordii, as well as their pure plantations in south subtropical China, the differences in microbial functional gene abundance related to soil carbon cycling (carbon fixation, carbon degradation and methane metabolism) between different soil layers of different stands, and the dominant soil environmental factors were analyzed. The results showed that the abundance of soil microbial carbon fixation functional genes (rcbL, MUT and PCCA) in the Pinus massoniana stand was significantly higher than that in the other two stands. This difference was attributed to higher soil total phosphorus (TP) content and significantly lower contents of microbial biomass carbon (MBC), easily oxidizable organic carbon (EOC), particulate organic carbon (POC), and recalcitrant organic carbon (ROC) (P < 0.05). The abundance of carbon degradation functional genes (MAN2C1 and bglB) was significantly higher in the Pinus massoniana stand than that in mixed plantation. These differences were mainly affected by the low content of soil organic carbon (SOC), MBC, dissolved organic carbon (DOC), EOC and ROC in Pinus massoniana plantation. The highest abundance of methane metabolism functional genes (pmoA-amoA, pmoB-amoB and pmoC-amoC) was observed in the Pinus massoniana plantation, which was related to significant negative effects of SOC, MBC, DOC, EOC, and ROC. Additionally, the abundance of microbial carbon cycle functional genes in all three planted forests increased with increasing soil depth, mainly due to the decrease in SOC, C/N ratio, MBC, DOC, EOC and ROC contents. Overall, the Pinus massoniana stand exhibited a relatively high potential for soil microbial carbon cycling, while the soil microbial carbon cycle potential in all three stands increased with soil depth. The composition of soil organic carbon was identified as an important factor regulating the difference in functional gene abundance of soil microbial carbon cycling in these plantations.

Key words: soil microbial carbon cycle functional genes, soil organic carbon components, metagenomic sequencing, planted forest, south subtropical China

摘要：

探究土壤微生物碳循环功能基因丰度对深刻理解土壤碳循环机制具有重要作用，然而土壤微生物碳循环功能基因丰度对不同人工林类型的响应特征尚不清楚。以南亚热带马尾松 (Pinus massoniana)-格木（Erythrophleum fordii）人工混交林及其纯林为研究对象，基于林地不同土层（0－20、20－40、40－60 cm）土壤样品的宏基因组测序数据以及土壤理化性质和有机碳组分，解析不同林分不同土层间土壤微生物碳循环（碳固定、碳降解和甲烷代谢）功能基因丰度的差异特征及其主导的土壤环境因子。结果表明：马尾松林土壤微生物碳固定功能基因（rcbL、MUT和PCCA）丰度显著高于其他2个林分，这与其土壤总磷（TP）含量较高且微生物生物量碳（MBC）、易氧化有机碳（EOC）、颗粒有机碳（POC）和惰性有机碳（ROC）含量显著较低的影响有关；土壤微生物碳降解功能基因（MAN2C1和bglB）丰度在马尾松林中显著高于混交林（P<0.05），主要受到马尾松林土壤有机碳（SOC）、MBC、可溶性有机碳（DOC）、EOC和ROC含量低的显著影响；马尾松林甲烷代谢功能基因（pmoA-amoA、pmoB-amoB和pmoC-amoC）丰度显著最高，这与土壤SOC、MBC、DOC、EOC和ROC的显著负作用有关。另外，3个人工林土壤微生物碳循环功能基因丰度基本随土壤深度增加而增加，主要与土壤SOC、C/N、MBC、DOC、EOC和ROC含量随土壤深度加深而降低密切相关。总之，马尾松林土壤微生物具有较高碳循环潜力，但3个林分土壤微生物碳循环潜力均随土壤深度增加而增强，土壤有机碳组分是主导3个人工林土壤微生物碳循环功能基因丰度差异的重要因素。

关键词: 土壤微生物碳循环功能基因, 土壤有机碳组分, 宏基因组测序, 人工林, 南亚热带

CLC Number:

QIN Jiaqi, XIAO Zhirou, MING Angang, ZHU Hao, TENG Jinqian, LIANG Zeli, TAO Yi, QIN Lin. Effect of Monoculture and Mixed Plantation with Coniferous and Broadleaved Tree Species on Soil Microbial Carbon Cycle Functional Gene Abundance[J]. Ecology and Environment, 2023, 32(10): 1719-1731.

秦佳琪, 肖指柔, 明安刚, 朱豪, 滕金倩, 梁泽丽, 陶怡, 覃林. 针阔人工混交林及其纯林对土壤微生物碳循环功能基因丰度的影响[J]. 生态环境学报, 2023, 32(10): 1719-1731.

Figures/Tables 7

References 50

[1]	BANI A, PIOLI S, VENTURA M, et al., 2018. The role of microbial community in the decomposition of leaf litter and deadwood[J]. Applied Soil Ecology, 126: 75-84. DOI URL
[2]	COOLEN M J, ORSI W D, 2015. The transcriptional response of microbial communities in thawing Alaskan permafrost soils[J]. Frontiers in Microbiology, 6: 197. DOI PMID
[3]	CUI L L, LI X, LIN J, et al., 2022. The mineralization and sequestration of soil organic carbon in relation to gully erosion[J]. Catena, 214: 106218. DOI URL
[4]	DENG J J, ZHU W X, ZHOU Y B, et al., 2019. Soil organic carbon chemical functional groups under different revegetation types are coupled with changes in the microbial community composition and the functional genes[J]. Forests, 10(3): 240. DOI URL
[5]	DENG L, SHANGGUAN Z-P, 2017. Afforestation drives soil carbon and nitrogen changes in China[J]. Land Degradation and Development, 28(1): 151-165. DOI URL
[6]	DING J J, ZHANG Y, WANG M, et al., 2015. Soil organic matter quantity and quality shape microbial community compositions of subtropical broadleaved forests[J]. Molecular Ecology, 24(20): 5175-5185. DOI PMID
[7]	HU Y G, ZHANG Z S, HUANG L, et al., 2019. Shifts in soil microbial community functional gene structure across a 61-year desert revegetation chronosequence[J]. Geoderma, 347: 126-134 DOI URL
[8]	HUANG J X, LIN T C, XIONG D C, et al., 2019. Organic carbon mineralization in soils of a natural forest and a forest plantation of southeastern China[J]. Geoderma, 344: 119-126. DOI URL
[9]	KUYPERS M M M, MARCHANT H K, KARTAL B, 2018. The microbial nitrogen-cycling network[J]. Nature Reviews Microbiology, 16(5): 263-276. DOI PMID
[10]	KOSUGI Y, MATSUURA N, LIANG Q C, et al., 2020. Wastewater treatment using the “sulfate reduction, denitrification anammox and partial nitrification (SRDAPN)” process[J]. Chemosphere, 256: 127092. DOI URL
[11]	LI H, YANG S, XU Z W, et al., 2017. Responses of soil microbial functional genes to global changes are indirectly influenced by aboveground plant biomass variation[J]. Soil Biology and Biochemistry, 104: 18-29. DOI URL
[12]	LIAO C, LI D, HUANG L, et al., 2020. Higher carbon sequestration potential and stability for deep soil compared to surface soil regardless of nitrogen addition in a subtropical forest[J]. Peer Journal, 8: e9128. DOI URL
[13]	LIAO J J, DOU Y X, YANG X, et al., 2023. Soil microbial community and their functional genes during grassland restoration[J]. Journal of Environmental Management, 325(Part A): 116488. DOI URL
[14]	LUO X Z, WEN D Z, HOU E Q, et al., 2022. Changes in the composition of soil microbial communities and their carbon-cycle genes following the conversion of primary broadleaf forests to plantations and secondary forests[J]. Land Degradation and Development, 33(6): 974-985. DOI URL
[15]	LYNN T M, GE T D, YUAN H Z, et al., 2017. Soil carbon-fixation rates and associated bacterial diversity and abundance in three natural ecosystems[J]. Microbial Ecology, 73(3): 645-657. DOI PMID
[16]	MA X Y, WANG T X, SHI Z, et al., 2022. Long-term nitrogen deposition enhances microbial capacities in soil carbon stabilization but reduces network complexity[J]. Microbiome, 10(1): 144. DOI
[17]	PAN Y D, BIRDSEY R A, FANG J Y, et al., 2011. A large and persistent carbon sink in the world’s forests[J]. Science, 333(6045): 988-993. DOI URL
[18]	PENG X Q, WANG W, 2016. Stoichiometry of soil extracellular enzyme activity along a climatic transect in temperate grasslands of northern China[J]. Soil Biology and Biochemistry, 98: 74-84. DOI URL
[19]	ROVIRA P, VALLEEJO V R, 2000. Examination of thermal and acid hydrolysis procedures in characterization of soil organic matter[J]. Communications in Soil Science and Plant Analysis, 31(1-2): 81-100. DOI URL
[20]	SOKOL N W, SANDERMAN J, BRADFORD M A, 2019. Pathways of mineral-associated soil organic matter formation: Integrating the role of plant carbon source, chemistry, and point of entry[J]. Global Change Biology, 25(1): 12-24. DOI PMID
[21]	TAN J L, WANG R, 2021. Research on evaluation and influencing factors of regional ecological efficiency from the perspective of carbon neutrality[J]. Journal of Environmental Management, 294: 113030. DOI URL
[22]	TRIVEDI P, DELGADO-BAQUERIZO M, TRIVEDI C, et al., 2016. Microbial regulation of the soil carbon cycle: evidence from gene-enzyme relationships[J]. Isme Journal, 10(11): 2593-2604. DOI PMID
[23]	WU H Q, DU S Y, ZHANG Y L, et al., 2019. Effects of irrigation and nitrogen fertilization on greenhouse soil organic nitrogen fractions and soil-soluble nitrogen pools[J]. Agricultural Water Management, 216: 415-424. DOI URL
[24]	WANG X Y, LI W, XIAO Y T, et al., 2021. Abundance and diversity of carbon-fixing bacterial communities in karst wetland soil ecosystems[J]. Catena, 204: 105418. DOI URL
[25]	WANG Y, ZHENG H, CHEN F L, et al., 2019. Forest restoration approaches affect soil compositions of lignin, substituted fatty acids, and lignin degradation-associated genes[J]. Applied Soil Ecology, 138: 213-219. DOI URL
[26]	WANG Y, ZHENG H, CHEN F L, et al., 2020. Stabilities of soil organic carbon and carbon cycling genes are higher in natural secondary forests than in artificial plantations in southern China[J]. Land Degradation and Development, 31(18): 2986-2995. DOI URL
[27]	XUE K, YUAN M M, SHI Z J, et al., 2016. Tundra soil carbon is vulnerable to rapid microbial decomposition under climate warming[J]. Nature Climate Change, 6(6): 595-600. DOI
[28]	YAN B S, SUN L P, LI J J, et al., 2020. Change in composition and potential functional genes of soil bacterial and fungal communities with secondary succession in Quercus liaotungensis forests of the Loess Plateau, western China[J]. Geoderma, 364: 114199. DOI URL
[29]	YANG X D, NI K, SHI Y Z, et al., 2023. Metagenomics reveals N-induced changes in carbon-degrading genes and microbial communities of tea (Camellia sinensis L.) plantation soil under long-term fertilization[J]. Science of the Total Environment, 856: 159231. DOI URL
[30]	YUE H W, WANG M M, WANG S P, et al., 2015. The microbe-mediated mechanisms affecting topsoil carbon stock in Tibetan grasslands[J]. Isme Journal, 9(9): 2012-2020. DOI PMID
[31]	ZHANG M K, HE Z L, 2004. Long-term changes in organic carbon and nutrients of an Ultisol under rice cropping in southeast China[J]. Geoderma, 118(3-4): 167-179. DOI URL
[32]	ZHENG Y, LIU X Z, ZHANG L M, et al., 2010. Do land utilization patterns affect methanotrophic communities in a Chinese upland red soil?[J]. Journal of Environmental Sciences, 22(12): 1936-1943. PMID
[33]	鲍士旦, 2000. 土壤农化分析[M]. 北京: 中国农业出版社: 1-120.
	BAO S D, 2000. Analysis for soil agriculture chemistry[M]. Beijing: China Agriculture Press: 1-120.
[34]	陈科屹, 王建军, 何友均, 等, 2022. 黑龙江大兴安岭重点国有林区森林碳储量及固碳潜力评估[J]. 生态环境学报, 31(9): 1725-1734. DOI
	CHEN K Y, WANG J J, HE Y J, et al., 2022. Estimations of forest carbon storage and carbon sequestration potential of key state-owned forest region in Daxing’anling, Heilongjiang Province[J]. Ecology and Environmental Sciences, 31(9): 1725-1734.
[35]	陈晓娟, 吴小红, 简燕, 等, 2014. 农田土壤自养微生物碳同化潜力及其功能基因数量、关键酶活性分析[J]. 环境科学, 35(3): 1144-1150.
	CHEN X J, WU X H, JIAN Y, et al., 2014. Carbon dioxide assimilation potential, functional gene amount and RubisCO activity of autotrophic microorganisms in agricultural soils[J]. Environmental Science, 35(3): 1144-1150.
[36]	胡明慧, 赵建琪, 王玄, 等, 2022. 自然增温对南亚热带森林土壤微生物群落与有机碳代谢功能基因的影响[J]. 生态学报, 42(1): 359-369.
	HU M H, ZHAO J Q, WANG X, et al., 2022. Effects of natural warming on soil microorganisms communities and functional genes of soil organic carbon metabolism in subtropical forests[J]. Acta Ecologicala Sinica, 42(1): 359-369.
[37]	李毳, 乔沙沙, 刘晋仙, 等, 2020. 温带亚高山针叶林土壤碳循环微生物群落的时空动态[J]. 中国环境科学, 40(10): 4540-4548.
	LI Q, QIAO S S, LIU J X, et al., 2020. Spatiotemporal dynamics of microbial communities involved in soil carbon cycling of subalpine temperate coniferous forest[J]. China Environmental Science, 40(10): 4540-4548.
[38]	梁艳, 明安刚, 何友均, 等, 2021. 南亚热带马尾松-红椎混交林及其纯林土壤细菌群落结构与功能[J]. 应用生态学报, 32(3): 878-886. DOI
	LIANG Y, MING A G, HE Y J, et al., 2021. Structure and function of soil bacterial communities in the monoculture and mixed plantation of Pinus massoniana and Castanopsis hystrix in southern subtropical China[J]. Chinese Journal of Applied Ecology, 32(3): 878-886. DOI
[39]	刘合明, 杨志新, 刘树庆, 2008. 不同粒径土壤活性有机碳测定方法的探讨[J]. 生态环境, 17(5): 2046-2049.
	LIU H M, YANG Z X, LIU S Q, 2008. Methods for determining labile orange matter in different sized soil particles of different soils[J]. Ecology and Environment, 17(5): 2046-2049.
[40]	刘茗, 曹林桦, 刘彩霞, 等, 2021. 亚热带4种典型森林植被土壤固碳细菌群落结构及数量特征[J]. 土壤学报, 58(4): 1028-1039.
	LIU M, CAO L H, LIU C X, et al., 2021. Characterization of population and community structure of carbon sequestration bacteria in soils under four types of forest vegetations typical of subtropical zone[J]. Acta Pedologica Sinica, 58(4): 1028-1039.
[41]	刘世荣, 代力民, 温远光, 等, 2015. 面向生态系统服务的森林生态系统经营: 现状、挑战与展望[J]. 生态学报, 35(1): 1-9.
	LIU S R, DAI L M, WEN Y G, et al., 2015. A review on forest ecosystem management towards ecosystem services: Status, challenges, and future perspectives[J]. Acta Ecologica Sinica, 35(1): 1-9.
[42]	刘世荣, 杨予静, 王晖, 2018. 中国人工林经营发展战略与对策: 从追求木材产量的单一目标经营转向提升生态系统服务质量和效益的多目标经营[J]. 生态学报, 38(1): 1-10.
	LIU S R, YANG Y J, WANG H, 2018. Development strategy and management countermeasures of planted forests in China: Transforming from timber-centered single objective management towards multi-purpose management for enhancing quality and benefits of ecosystem services[J]. Acta Ecologica Sinica, 38(1): 1-10. DOI URL
[43]	刘洋荧, 王尚, 厉舒祯, 等, 2017. 基于功能基因的微生物碳循环分子生态学研究进展[J]. 微生物学通报, 44(7): 1676-1689.
	LIU Y Y, WANG S, LI S Z, et al., 2017. Advances in molecular ecology on microbial functional genes of carbon cycle[J]. Microbiology China, 44(7): 1676-1689.
[44]	鲁如坤, 2000. 土壤农业化学分析方法[M]. 北京: 中国农业科技出版社:60-73.
	LU R K, 2000. Methods of agrochemical analysis of soil[M]. 北Beijing: China Agricultural Science and Technology Press:60-73.
[45]	罗达, 史作民, 唐敬超, 等, 2014. 南亚热带乡土树种人工纯林及混交林土壤微生物群落结构[J]. 应用生态学报, 25(9): 2543-2550.
	LUO D, SHI Z M, TANG J C, et al., 2014. Soil microbial community structure of monoculture and mixed plantation stands of native tree species in south subtropical China[J]. Chinese Journal of Applied Ecology, 25(9): 2543-2550.
[46]	宋瑞朋, 杨起帆, 郑智恒, 等, 2022. 3种林下植被类型对杉木人工林土壤有机碳及其组分特征的影响[J]. 生态环境学报, 31(12): 2283-2291. DOI
	SONG R P, YANG Q F, ZHENG Z H, et al., 2022. Effects of three understory vegetation types on soil organic carbon and its components in Cunninghamia lanceolata plantation[J]. Ecology and Environmental Sciences, 31(12): 2283-2291.
[47]	王芸, 郑华, 陈法霖, 等, 2012. 湿地松和马尾松人工林土壤甲烷代谢微生物群落的结构特征[J]. 生态学报, 32(8): 2458-2465.
	WANG Y, ZHENG H, CHEN F L, et al., 2012. Research of methane metabolic microbial community in soils of slash pine plantation and masson pine plantation[J]. Acta Ecologica Sinica, 32(8): 2458-2465. DOI URL
[48]	吴金水, 林启美, 黄巧云, 等, 2006. 土壤微生物生物量测定方法及其应用[M]. 北京: 气象出版社:57-60.
	WU J S, LIN Q M, HUANG Q Y, et al., 2006. Methods for determination of soil microbial biomass and their applications[M]. Beijing: China Agricultural Science and Technology Press: 57-60.
[49]	袁红朝, 秦红灵, 刘守龙, 等, 2011. 固碳微生物分子生态学研究[J]. 中国农业科学, 44(14): 2951-2958. DOI
	YUAN H C, QIN H L, LIU S L, et al., 2011. Advances in research of molecular ecology of carbon fixation microorganism[J]. Scientia Agricultura Sinica, 44(14): 2951-2958.
[50]	赵姣, 马静, 朱燕峰, 等, 2023. 植被类型对黄土高原露采矿山复垦土壤碳循环功能基因的影响[J]. 环境科学, 44(6): 3386-3395.
	ZHAO J, MA J, ZHU Y F, et al., 2023. Effects of vegetation types on carbon cycle functional genes in reclaimed soil from open pit mines in the Loess Plateau[J]. Environmental Science, 44(6): 3386-3395.

碳循环功能基因	基因	KO编号
碳固定
卡尔文循环
核酮糖-二磷酸羧化酶大链	rbcL	K01601
核糖5-磷酸异构酶	rpiA	K01807
磷酸甘油酸激酶	PGK	K00927
还原乙酰辅酶A途径
乙酰辅酶a合成酶	acs	K01895
乙酰乙酰辅酶a合成酶	acsA	K01907
还原三羧酸循环
2-氧代戊二酸/2-氧酸铁氧还蛋白氧化还原酶亚单位	korA	K00174
2-氧代戊二酸/2-氧代酸铁氧还蛋白氧化还原酶亚基	korB	K00175
3-烃基丙酸双循环
乙酰辅酶a羧化酶羧基转移酶亚单位	accA	K01962
丙酰辅酶a羧化酶α链	PCCA	K01965
3-烃基丙酸/4-烃基丁酸循环
甲基丙二酰辅酶a变位酶	MUT	K01847
二羧酸/4-烃基丁酸循环
磷酸烯醇丙酮酸羧化酶	ppc	K01595
丙酮酸，正磷酸二激酶	ppdK	K01006
碳降解
淀粉
Α-淀粉酶	amyA	K01176
支链淀粉酶	pulA	K01200
4-α-葡聚糖转移酶	malQ	K00705
果胶
果胶酯酶果胶裂解酶	pectinesterase pel	K01051 K01728
半纤维素
Α-L-阿拉伯呋喃糖苷酶	abfA	K01209
木酮糖激酶	xylB	K00854
Α-甘露糖苷酶	MAN2C1	K01191
木糖异构酶	xylA	K01805
纤维素
内切葡聚糖酶	endoglucanase	K01179
Β-葡萄糖苷酶	bglB	K05350
Β-葡萄糖苷酶	bglX	K05349
几丁质
几丁质酶	chitinase	K01183
N-乙酰氨基葡萄糖-6-磷酸脱乙酰酶	nagA	K01443
木质素
儿茶酚2, 3-双加氧酶甲烷代谢	catE	K07104
甲烷氧化
甲烷/氨单加氧酶亚单位	pmoA-amoA	K10944
甲烷/氨单加氧酶亚单位	pmoB-amoB	K10945
甲烷/氨单加氧酶亚单位	pmoC-amoC	K10946
甲烷生成
四氢甲蝶呤S-甲基转移酶亚基	mtrA	K00577

碳循环功能基因	基因	KO编号
碳固定
卡尔文循环
核酮糖-二磷酸羧化酶大链	rbcL	K01601
核糖5-磷酸异构酶	rpiA	K01807
磷酸甘油酸激酶	PGK	K00927
还原乙酰辅酶A途径
乙酰辅酶a合成酶	acs	K01895
乙酰乙酰辅酶a合成酶	acsA	K01907
还原三羧酸循环
2-氧代戊二酸/2-氧酸铁氧还蛋白氧化还原酶亚单位	korA	K00174
2-氧代戊二酸/2-氧代酸铁氧还蛋白氧化还原酶亚基	korB	K00175
3-烃基丙酸双循环
乙酰辅酶a羧化酶羧基转移酶亚单位	accA	K01962
丙酰辅酶a羧化酶α链	PCCA	K01965
3-烃基丙酸/4-烃基丁酸循环
甲基丙二酰辅酶a变位酶	MUT	K01847
二羧酸/4-烃基丁酸循环
磷酸烯醇丙酮酸羧化酶	ppc	K01595
丙酮酸，正磷酸二激酶	ppdK	K01006
碳降解
淀粉
Α-淀粉酶	amyA	K01176
支链淀粉酶	pulA	K01200
4-α-葡聚糖转移酶	malQ	K00705
果胶
果胶酯酶果胶裂解酶	pectinesterase pel	K01051 K01728
半纤维素
Α-L-阿拉伯呋喃糖苷酶	abfA	K01209
木酮糖激酶	xylB	K00854
Α-甘露糖苷酶	MAN2C1	K01191
木糖异构酶	xylA	K01805
纤维素
内切葡聚糖酶	endoglucanase	K01179
Β-葡萄糖苷酶	bglB	K05350
Β-葡萄糖苷酶	bglX	K05349
几丁质
几丁质酶	chitinase	K01183
N-乙酰氨基葡萄糖-6-磷酸脱乙酰酶	nagA	K01443
木质素
儿茶酚2, 3-双加氧酶甲烷代谢	catE	K07104
甲烷氧化
甲烷/氨单加氧酶亚单位	pmoA-amoA	K10944
甲烷/氨单加氧酶亚单位	pmoB-amoB	K10945
甲烷/氨单加氧酶亚单位	pmoC-amoC	K10946
甲烷生成
四氢甲蝶呤S-甲基转移酶亚基	mtrA	K00577

土层	林分类型	w_(MBC)/(mg∙kg⁻¹)	w_(DOC)/(mg∙kg⁻¹)	w_(EOC)/(mg∙kg⁻¹)	w_(POC)/(mg∙kg⁻¹)	w_(ROC)/(mg∙kg⁻¹)
0－20 cm	PMP	11.80±1.80Ab	17.67±3.68Aa	301.45±38.14Aa	1091.00±86.09ABb	12802.03±1472.29Ab
	EFP	19.01±3.99Bb	22.51±11.12Aa	586.24±28.39Cb	1235.58±210.61Bb	13574.62±1225.81Ab
	MPE	16.36±0.74ABb	21.29±4.50Aa	436.65±65.99Bb	837.25±96.56Ab	13238.25±611.61Aab
20－40 cm	PMP	11.56±2.03Ab	14.45±4.24Aa	266.81±43.12Aa	573.92±122.68Aa	7761.56±1254.92Aa
	EFP	14.55±6.30Aab	15.69±3.26Aa	282.44±58.75Aa	1296.46±101.68Bb	10663.02±1148.89Ba
	MPE	13.73±3.99Ab	17.17±4.99Aa	175.81±50.45Aa	478.28±32.78Aa	13611.27±417.10Cb
40－60 cm	PMP	6.37±0.83Aa	15.47±3.28Aa	265.60±44.39Aa	677.66±43.70Aa	8484.44±464.20Aa
	EFP	9.03±0.59Ba	10.79±2.30Aa	260.93±44.75Aa	878.92±143.22Ba	10793.59±1019.76Aa
	MPE	6.18±0.77Aa	13.56±6.45Aa	210.74±59.12Aa	588.06±53.65Aa	10879.13±2044.52Aa
双因素方差分析	林分	0.025^{* 1)}	NS	0.000^**	0.000^**	0.000^**
	土层	0.000^{** 2)}	0.035^*	0.000^**	0.000^**	0.000^**
	林分×土层	NS³⁾	NS	0.001^**	0.001^**	0.013^*

土层	林分类型	w_(MBC)/(mg∙kg⁻¹)	w_(DOC)/(mg∙kg⁻¹)	w_(EOC)/(mg∙kg⁻¹)	w_(POC)/(mg∙kg⁻¹)	w_(ROC)/(mg∙kg⁻¹)
0－20 cm	PMP	11.80±1.80Ab	17.67±3.68Aa	301.45±38.14Aa	1091.00±86.09ABb	12802.03±1472.29Ab
	EFP	19.01±3.99Bb	22.51±11.12Aa	586.24±28.39Cb	1235.58±210.61Bb	13574.62±1225.81Ab
	MPE	16.36±0.74ABb	21.29±4.50Aa	436.65±65.99Bb	837.25±96.56Ab	13238.25±611.61Aab
20－40 cm	PMP	11.56±2.03Ab	14.45±4.24Aa	266.81±43.12Aa	573.92±122.68Aa	7761.56±1254.92Aa
	EFP	14.55±6.30Aab	15.69±3.26Aa	282.44±58.75Aa	1296.46±101.68Bb	10663.02±1148.89Ba
	MPE	13.73±3.99Ab	17.17±4.99Aa	175.81±50.45Aa	478.28±32.78Aa	13611.27±417.10Cb
40－60 cm	PMP	6.37±0.83Aa	15.47±3.28Aa	265.60±44.39Aa	677.66±43.70Aa	8484.44±464.20Aa
	EFP	9.03±0.59Ba	10.79±2.30Aa	260.93±44.75Aa	878.92±143.22Ba	10793.59±1019.76Aa
	MPE	6.18±0.77Aa	13.56±6.45Aa	210.74±59.12Aa	588.06±53.65Aa	10879.13±2044.52Aa
双因素方差分析	林分	0.025^{* 1)}	NS	0.000^**	0.000^**	0.000^**
	土层	0.000^{** 2)}	0.035^*	0.000^**	0.000^**	0.000^**
	林分×土层	NS³⁾	NS	0.001^**	0.001^**	0.013^*

Effect of Monoculture and Mixed Plantation with Coniferous and Broadleaved Tree Species on Soil Microbial Carbon Cycle Functional Gene Abundance

针阔人工混交林及其纯林对土壤微生物碳循环功能基因丰度的影响

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 50

Related Articles 0

Recommended Articles

Metrics

Comments