生态环境学报 ›› 2022, Vol. 31 ›› Issue (11): 2225-2233.DOI: 10.16258/j.cnki.1674-5906.2022.11.014

• 研究论文 • 上一篇    下一篇

赤霉素浸种对2种矿区修复先锋植物种子萌发和幼苗生长的影响

俞龙生1,2(), 李卫2, 许铭宇2,3,*(), 林泽帆1   

  1. 1.广州草木蕃环境科技有限公司/广东省湿地修复工程技术研究中心，广东 广州510520
    2.仲恺农业工程学院园艺园林学院，广东 广州 510225
    3.华南理工大学环境与能源学院，广东 广州 510006
  • 收稿日期:2022-09-27 出版日期:2022-11-18 发布日期:2022-12-22
  • 通讯作者: *许铭宇（1991年生），男，博士研究生，主要从事土地复垦与生态修复。E-mail: xmy4200@126.com
  • 作者简介:俞龙生（1984年生），男，高级工程师，主要从事土壤修复工程技术研究。E-mail: sysulss@163.com
  • 基金资助:
    广东省重点领域研发计划项目(2019B110207001);广州市重点研发计划项目(202206010176);韶关市生态环境局项目(ZL17GZ016)

Effect of Gibberellin Soaking on Seed Germination and Seedling Growth of Two Pioneer Plants for Ecological Restoration in Mining Areas

YU Longsheng1,2(), LI Wei2, XU Mingyu2,3,*(), LIN Zefan1   

  1. 1. Guangzhou CAOMUFAN Ecological Research Co., Ltd. & Research Center of Engineering Technology for Wetland Restoration of Guangdong Province, Guangzhou 510520, P. R. China
    2. College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, P. R. China
    3. School of Environment and Energy, South China University of Technology, Guangzhou 510006, P. R. China
  • Received:2022-09-27 Online:2022-11-18 Published:2022-12-22

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摘要：

豆科植物是金属矿区生态修复中常用物种，由于种子种皮致密、透水性较差导致发芽受阻而影响植物修复效果，为解决豆科植物种子硬实的问题，以猪屎豆（Crotalaria pallida Ait.）、黄槐决明（Cassia surattensis Burm. F.）种子为试验材料，采用200 mg·L-1的赤霉素和蒸馏水分别浸种12、24、36 h，以研究赤霉素浸种对供试种子萌发和幼苗生长的影响。结果表明，（1）200 mg·L-1赤霉素浸种能够促进猪屎豆种子萌发，其发芽率、发芽势、发芽指数等数据均高于对照组。最佳的浸种时长为12 h，其发芽率指标显著高于对照组和蒸馏水浸种处理。但随着浸种时间延长，促进发芽的效果呈现先降后升的趋势，浸种36 h对猪屎豆种子活力有明显提升作用。（2）赤霉素和蒸馏水浸种均能促进黄槐决明种子萌发，赤霉素最佳的浸种时长为24 h，其发芽率指标显著高于对照组和蒸馏水浸种处理，随着浸种时间延长促进发芽的效果呈现降低趋势。（3）赤霉素浸种处理对猪屎豆幼苗生物量指标促进效果不明显，高浓度的赤霉素浸种对幼苗生长反而有抑制作用，选用赤霉素作为猪屎豆的浸种剂时，需要更加注意剂量和时间的选取。（4）赤霉素浸种对黄槐决明幼苗苗长、根长、根冠比等生物量指标有一定促进作用，其中浸种24 h对苗长、根长都有明显提升作用。综上，采用赤霉素浸种适宜时间对两种供试植物种子萌发都有不同的促进作用，但浸种对幼苗生物量影响不一；在矿山生态修复实践中，可结合工期和成本等因素采用更加细致的梯度试验综合优选浸种试剂、剂量和浸种时间，从而达到设计预期修复效果。

关键词: 矿业废弃地, 植物修复, 豆科植物, 赤霉素, 浸种时间

Abstract:

In order to solve the hardness problem of Leguminosae seeds, two pioneer plants for mine ecological restoration, Crotalaria pallida Ait. and Cassia surattensis Burm. F. were chosen for experimental materials. The seeds were soaked in 200 mg·L-1 gibberellin and distilled water for 12, 24, and 36 h, respectively, to study the effects of gibberellin soaking on the germination of the tested seeds and seedling growth. The results showed that (1) with 200 mg·L-1 Gibberellin soaking for 12 h, the germination rate of C. pallida was significantly higher than that in CK and distilled water seed soaking treatment. However, with more soaking time, the effects of promoting germination were not obvious, and Gibberellin soaking for 36 h could improve the seed vitality and other indices. (2) Both Gibberellin and distilled water soaking could promote seed germination rates of C. surattensis. The best seed soaking time of Gibberellin was 24 h, and the germination rate was significantly higher than that in CK and distilled water soaking treatment. The effects of germination promoting were decreased with soaking time. (3) The seed soaking with Gibberellin could not promote the biomass indices of C. pallida obviously, while the high concentration of Gibberellin should inhibit the seedling growth. (4) The seed soaking with Gibberellin could promote the biomass indices of C. surattensis, e.g., the seedling length and root length were improved under Gibberellin soaking for 24 h. In summary, the seed hardness of two test plants could be broken by Gibberellin soaking for an appropriate period of time. The reagent, concentration and soaking time should all be considered in terms of economic and time costs in ecological restoration of mining area.

Key words: mining waste land, phytoremediation, Leguminosae, Gibberellin, soaking time

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