Effect of Ozone Concentration Increasing Near the Ground on Antioxidant System of Parent and Offspring Soybean Leaves

doi:10.16258/j.cnki.1674-5906.2021.11.010

Ecology and Environment ›› 2021, Vol. 30 ›› Issue (11): 2195-2203.DOI: 10.16258/j.cnki.1674-5906.2021.11.010

• Research Articles • Previous Articles Next Articles

Effect of Ozone Concentration Increasing Near the Ground on Antioxidant System of Parent and Offspring Soybean Leaves

ZHOU Yingtong(), WANG Yan, SUN Mingyu, SAN Yu, YAO Xingzhou, ZHAO Tianhong

College of Agronomy, Shenyang Agricultural University, Shenyang 110000, China

Received:2021-06-11 Online:2021-11-18 Published:2021-12-29
Contact: ZHAO Tianhong

近地层臭氧浓度升高对亲子代大豆叶片抗氧化系统的影响

周映彤(), 王岩, 孙铭禹, 伞昱, 姚星州, 赵天宏

沈阳农业大学农学院,辽宁沈阳 110000

通讯作者: 赵天宏
作者简介:周映彤（1997年生）,女,硕士研究生,研究方向为农业生态系统生态学。E-mail: 1121705386@qq.com
基金资助:
国家自然科学基金项目(31570404)

Abstract

Abstract:

In order to explore the effect of ozone stress on the antioxidant ability of different generations of Glycine max, three O₃ fumigation concentration gradients [CK: control, 45 nL∙L^-1 O₃; T1:(80±10) nL∙L^-1 O₃; T2: (120±10) nL∙L^-1 O₃] were used to treat soybean in open top chamber (OTCs), and then copied the species after the ozone stress and studied the difference ability of antioxidant. The results showed that (1) the relative conductivity in soybean leaves increased significantly between flowering and pod periods compared with CK treatment (P<0.05). MDA content increased and had a significantly difference during the branching, flowering stage and T2 condition (P<0.05) by 32.9% and 37.0% with CK treatment, respectively. (2) The O₂^∙- production rate increased, and both S1 and S2 showed significantly different level except for branch T1 conditions (P<0.05); the H₂O₂ content increased, and the S1 and S2 increased significantly at T2 during the branching and flowering stages, reaching 88.2% and 92.4% respectively. (3) SOD, CAT activity gradually decreased and POD activity gradually increased, which showed a significantly decline in all three stages compared with the CK treatment (P<0.05). (4) In the later stage, the ROS content and generation rate in offspring soybean leaves were higher than the parental; the oxidase activity in offspring soybean leaves was lower than the parental, and reached significantly levels under T2 condition (P<0.05). (5) The degree of membrane lipid peroxidation, active oxygen content and production rate were significantly positively correlated between parent and offspring soybeans (P<0.01), significantly negatively associated with SOD, CAT activity (P<0.01) and unrelated to POD activity (P>0.05). The results show that increased ozone concentration significantly inhibit the antioxidant capacity of soybeans, and the offspring slightly stronger than the parent, which indicate that it is also affected by genetic factors.

Key words: increased ozone concentration, active oxygen, anti-oxidation, soybean, parent and offspring

摘要：

为探究臭氧胁迫对不同世代大豆（Glycine max）抗氧化能力的影响,以栽培大豆“铁丰29号”为试验材料,在开顶式气室（OTCs）中,采用盆栽试验方法,设置3个O₃熏蒸体积分数梯度[CK：对照,45 nL∙L^-1 O₃;T1：(80±10) nL∙L^-1 O₃;T2：(120±10) nL∙L^-1 O₃],并将受胁迫后的子代复种,研究臭氧浓度升高条件下大豆亲代和子代抗氧化能力的差异。结果表明,（1）随着臭氧体积分数增加,开花期和结荚期亲子代大豆叶片相对电导率较CK显著增加（P<0.05）;MDA含量均增加,且在分枝期和开花期的T2处理下,与CK之间达显著差异（P<0.05）,其增加幅度分别为32.9%、37.0%。（2）随着臭氧体积分数增加,O₂^∙-产生速率加快,除分枝期T1条件的其他处理外,S1和S2较CK都表现出显著差异水平（P<0.05）;H₂O₂含量增大,在分枝期和开花期的T2条件下,S1、S2较CK上升幅度显著,分别达到88.2%、92.4%（P<0.05）。（3）SOD、CAT活性逐渐减弱,POD活性逐步增强。与对照相比,亲子代在3个时期均有显著下降的趋势（P<0.05）。（4）在胁迫后期,子代大豆叶片的ROS含量、产生速率高于亲代,抗氧化酶活性低于亲代,且在T2处理下达到显著水平（P<0.05）。（5）亲、子代大豆的膜脂过氧化程度、活性氧含量和产生速率两两之间均呈极显著正相关（P<0.01）,与SOD、CAT活性均极显著负相关（P<0.01）,与POD活性呈不相关关系（P>0.05）。试验表明,臭氧体积分数升高对亲子代大豆的抗氧化能力有明显的抑制作用,且对子代的抑制作用略强于亲代,说明同时受到了遗传因素的影响。

关键词: 臭氧浓度升高, 活性氧, 抗氧化, 大豆, 亲子代

CLC Number:

X171.5

ZHOU Yingtong, WANG Yan, SUN Mingyu, SAN Yu, YAO Xingzhou, ZHAO Tianhong. Effect of Ozone Concentration Increasing Near the Ground on Antioxidant System of Parent and Offspring Soybean Leaves[J]. Ecology and Environment, 2021, 30(11): 2195-2203.

周映彤, 王岩, 孙铭禹, 伞昱, 姚星州, 赵天宏. 近地层臭氧浓度升高对亲子代大豆叶片抗氧化系统的影响[J]. 生态环境学报, 2021, 30(11): 2195-2203.

Figures/Tables 6

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因素 Factor	自由度 df	显著性Significance
因素 Factor	自由度 df	外渗电导率Relative electrical conductivity	MDA含量 MDA content	H₂O₂含量H₂O₂ content	O₂^∙-产生速率O₂^∙- production	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
O₃	2	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01
Generation	1	<0.01	0.049	0.050	<0.01	<0.01	0.016	0.010
Stage	2	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01
O₃×Generation	2	0.674	0.875	0.431	0.062	0.294	0.048	0.405
O₃×Stage	4	0.023	0.981	0.764	0.059	<0.01	<0.01	0.696
Generation×Stage	2	0.010	0.818	0.279	0.439	<0.01	0.878	0.238
O₃×Generation×Stage	4	0.080	0.732	0.998	0.970	0.544	0.836	0.583

因素 Factor	自由度 df	显著性Significance
因素 Factor	自由度 df	外渗电导率Relative electrical conductivity	MDA含量 MDA content	H₂O₂含量H₂O₂ content	O₂^∙-产生速率O₂^∙- production	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
O₃	2	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01
Generation	1	<0.01	0.049	0.050	<0.01	<0.01	0.016	0.010
Stage	2	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01	<0.01
O₃×Generation	2	0.674	0.875	0.431	0.062	0.294	0.048	0.405
O₃×Stage	4	0.023	0.981	0.764	0.059	<0.01	<0.01	0.696
Generation×Stage	2	0.010	0.818	0.279	0.439	<0.01	0.878	0.238
O₃×Generation×Stage	4	0.080	0.732	0.998	0.970	0.544	0.836	0.583

指标 Index	相关系数 (r) Correlation coefficient
指标 Index	外渗电导率 Relative electrical conductivity	MDA含量 MDA content	H₂O₂含量 H₂O₂ content	O₂^∙-产生速率 O₂^∙- production	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
外渗电导率 Relative electrical conductivity	1	0.667**	0.584**	0.660**	-0.636**	-0.533**	0.412*
MDA含量 MDA content		1	0.849**	0.814**	-0.713**	-0.686**	0.018
H₂O₂含量 H₂O₂ content			1	0.837**	-0.807**	-0.851**	-0.134
O₂^∙-产生速率 O₂^∙- production				1	-0.802**	-0.799**	-0.126
SOD活性 SOD activity					1	0.856**	0.296
CAT活性 CAT activity						1	0.285
POD活性 POD activity							1

指标 Index	相关系数 (r) Correlation coefficient
指标 Index	外渗电导率 Relative electrical conductivity	MDA含量 MDA content	H₂O₂含量 H₂O₂ content	O₂^∙-产生速率 O₂^∙- production	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
外渗电导率 Relative electrical conductivity	1	0.667**	0.584**	0.660**	-0.636**	-0.533**	0.412*
MDA含量 MDA content		1	0.849**	0.814**	-0.713**	-0.686**	0.018
H₂O₂含量 H₂O₂ content			1	0.837**	-0.807**	-0.851**	-0.134
O₂^∙-产生速率 O₂^∙- production				1	-0.802**	-0.799**	-0.126
SOD活性 SOD activity					1	0.856**	0.296
CAT活性 CAT activity						1	0.285
POD活性 POD activity							1

指标 Index	相关系数 (r) Correlation coefficient
指标 Index	外渗电导率 Relative electrical conductivity	MDA含量 MDA content	H₂O₂含量 H₂O₂ content	O₂^∙-产生速率 O₂^∙- production	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
外渗电导率 Relative electrical conductivity	1	0.702**	0.596**	0.644**	-0.626**	-0.616**	0.506*
MDA含量 MDA content		1	0.723**	0.724**	-0.671**	-0.743**	0.047
H₂O₂含量 H₂O₂ content			1	0.728**	-0.632**	-0.751**	-0.138
O₂^∙-产生速率 O₂^∙- production				1	-0.848**	-0.892**	-0.206
SOD活性 SOD activity					1	0.825**	0.056
CAT活性 CAT activity						1	0.259
POD活性 POD activity							1

Effect of Ozone Concentration Increasing Near the Ground on Antioxidant System of Parent and Offspring Soybean Leaves

近地层臭氧浓度升高对亲子代大豆叶片抗氧化系统的影响

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[1]	LI Chengcheng, ZHANG Zirui, SONG Xiaoxuan, KONG Juanjuan, HAN Yang, RUAN Yanan. Effects of Ozone Stress on Antioxidant Metabolism and Reproductive Growth of Soybean [J]. Ecology and Environment, 2022, 31(7): 1383-1392.
[2]	LIU Jiang, ZHU Lijie, ZHANG Kai, WANG Xiaoming, WANG Liwei, GAO Xining. Effects of Drought Stress/Rewatering on Photosynthetic Characteristics and Yield of Soybean at Different Growth Stages [J]. Ecology and Environment, 2022, 31(2): 286-296.