无机砷短期胁迫对铜藻幼苗氧化损伤、抗氧化酶及抗氧化物的影响

doi:10.16258/j.cnki.1674-5906.2021.05.016

生态环境学报 ›› 2021, Vol. 30 ›› Issue (5): 1034-1041.DOI: 10.16258/j.cnki.1674-5906.2021.05.016

无机砷短期胁迫对铜藻幼苗氧化损伤、抗氧化酶及抗氧化物的影响

张鹏¹(), 刘玮²^,^*(), 王铁杆¹, 钟晨辉³, 陶月良⁴

1.浙江省海洋水产养殖研究所/浙江省近岸水域生物资源开发与保护重点实验室,浙江温州 325000
2.山东省海洋生物研究院藻类研究中心,山东青岛 266104
3.福建省水产研究所/福建省海洋生物增养殖与高值化利用重点实验室,福建厦门 361000
4.温州大学生命与环境科学学院,浙江温州 325000

收稿日期:2020-12-14 出版日期:2021-05-18 发布日期:2021-08-06
通讯作者: * 刘玮（1981年生）,助理研究员,博士,主要从事大型海藻增殖及生理生态研究。E-mail:liuwei_mbi@163.com
作者简介:张鹏（1982年生）,男,高级工程师,硕士,主要从事大型藻类生理生态和遗传育种。E-mail:zhangpeng20011918@163.com
基金资助:
农业部藻类产业技术体系(CARS-50);国家重点研发计划项目(2018YFC1406300)

Impacts of Short-term Inorganic Arsenic Stress on Oxidative Damage, Antioxidant Enzymes and Antioxidant in Germlings of Sargassum horneri

ZHANG Peng¹(), LIU Wei²^,^*(), WANG Tiegan¹, ZHONG Chenhui³, TAO Yueliang⁴

1. Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource/Zhejiang Mariculture Research Institute, Wenzhou 325000, China
2. Algae Research Center, Marine Biology Institute of Shandong Province, Qingdao 266104, China
3. Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province/Fisheries Research Institute of Fujian, Xiamen 361000, China
4. Wenzhou University, Wenzhou 325000, China

Received:2020-12-14 Online:2021-05-18 Published:2021-08-06

摘要/Abstract

摘要：

为探讨无机砷胁迫对铜藻（Sargassum horneri）的生理影响,对铜藻幼苗在不同浓度（0、50、100、150、200 mmol∙L^-1）的As³⁺、As⁵⁺短期胁迫条件下其氧化损伤、抗氧化酶、抗氧化物等8项生理指标进行了测定。结果显示,随着As³⁺、As⁵⁺浓度的增加,铜藻体内的氧自由基（Oxygen free radical,OFR）产生速率、丙二醛（Malondialdehyde,MDA）含量明显升高,铜藻细胞损伤率也同时上升。As³⁺、As⁵⁺对铜藻的半效应浓度（Median effect concentration,EC₅₀）分别为50.1、116.3 mmol∙L^-1。随As处理浓度升高,藻体超氧化物歧化酶（Superoxide dismutase,SOD）和过氧化物酶（Peroxidase,POD）活性呈上升趋势,而过氧化氢酶（Catalase,CAT）活性则无明显变化。谷胱甘肽（Glutathione,GSH）含量随As³⁺浓度的增加呈先升后降,而随As⁵⁺浓度的增加总体呈上升趋势。非蛋白巯基化合物（Non-protein thiol,NPT）含量随As³⁺、As⁵⁺浓度的升高而先升后降。对铜藻抗氧化系统主成分分析表明,As³⁺ 50mmol∙L^-1及As⁵⁺ 100mmol∙L^-1实验组的铜藻抗氧化综合得分较高;随着As³⁺、As⁵+浓度的升高,抗氧化物（GSH、NPT）对铜藻抗氧化系统的贡献减弱,而抗氧化酶（SOD、POD）对铜藻抗氧化系统的贡献相对增强。

关键词: 价态, 抗氧化酶, 抗氧化物, 主成分分析, 半效应浓度

Abstract:

To explore the impact of inorganic arsenic stress on physiology of Sargassum horneri, the 8 physiological indexes about oxidative damage, antioxidant enzymes and antioxidant were detected while the germlings of S. horneri were shortly treated with different concentrations (0, 50, 100, 150, 200 mmol∙L^-1) of As³⁺ and As⁵⁺, respectively. The results showed that rate of oxygen free radical (OFR) production and content of malondialdehyde (MDA) in S. horneri seedlings increased significantly with increasing concentration of As³⁺ or As⁵⁺, and the damage rate of cellular was also elevated simultaneously. The medium effect concentrations (EC₅₀) of As³⁺ and As⁵⁺ to S. horneri were 50.1 and 116.3 mmol∙L^-1, respectively. Activities of superoxide dismutase (SOD) and peroxidase (POD) showed an upward trend in general, while catalase (CAT) varied insignificantly. The content of glutathione (GSH) increased first and then decreased with increase of As³⁺ concentration, whereas it showed an overall upward trend with increase of As⁵⁺ concentration. The content of non-protein thiol compound (NPT) tended to ascend first, then reduce with increasing As³⁺ or As⁵⁺ concentration. The principal component analysis of the S. horneri antioxidant system showed that the 50 mmol∙L^-1 (As³⁺) and 100 mmol∙L^-1 (As⁵⁺) treatments had higher comprehensive score of antioxidant system rather than others. As the concentration of As³⁺ and As⁵⁺ increased, the contribution of antioxidants (GSH, NPT) to the S. horneri antioxidant system weakened, while the contribution of antioxidase (SOD, POD) to the antioxidant system increased relatively.

Key words: valence state, antioxidase, antioxidant, principal component analysis, medium effect concentration

中图分类号:

X171.5

张鹏, 刘玮, 王铁杆, 钟晨辉, 陶月良. 无机砷短期胁迫对铜藻幼苗氧化损伤、抗氧化酶及抗氧化物的影响[J]. 生态环境学报, 2021, 30(5): 1034-1041.

ZHANG Peng, LIU Wei, WANG Tiegan, ZHONG Chenhui, TAO Yueliang. Impacts of Short-term Inorganic Arsenic Stress on Oxidative Damage, Antioxidant Enzymes and Antioxidant in Germlings of Sargassum horneri[J]. Ecology and Environment, 2021, 30(5): 1034-1041.

图/表 7

图1 不同浓度砷胁迫下铜藻相对电导率n=3。不同小写字母代表实验组存在显著差异（α=0.05）。下同

Fig. 1 Relative conductivity changes of S. horneri under different concentrations of arsenic stressn=3. Different lowercase letters indicate significant differences (α=0.05) between treatments. The same below

图2 铜藻相对电导率的Logistic拟合曲线

Fig. 2 Logistic fitting curves based on relative conductivity of S. horneri

图3 不同砷浓度下铜藻OFR和MDA变化情况

Fig. 3 Variation of OFR and MDA in S. horneri under different concentrations of arsenic

图4 不同砷浓度下SOD、POD和CAT的活性变化

Fig. 4 Activities of SOD, POD and CAT varied in S. horneri under different concentrations of arsenic

图5 不同砷浓度下GSH和NPT的浓度变化

Fig. 5 Variations of GSH and NPT content in S. horneri under different concentrations of arsenic

表1 砷胁迫实验组抗氧化综合得分排序

Table 1 Ranking arsenic stress treatments with antioxidant comprehensive score

砷胁迫实验组 As stress treatment	综合得分 Composite Score	排序 Ranking
(As³⁺, As⁵⁺) 0 mmol∙L^-1	-2.13	9
(As³⁺) 50 mmol∙L^-1	1.05	2
(As³⁺) 100 mmol∙L^-1	0.38	4
(As³⁺) 150 mmol∙L^-1	0.15	5
(As³⁺) 200 mmol∙L^-1	-0.71	8
(As⁵⁺) 50 mmol∙L^-1	-0.06	6
(As⁵⁺) 100 mmol∙L^-1	1.09	1
(As⁵⁺) 150 mmol∙L^-1	0.46	3
(As⁵⁺) 200 mmol∙L^-1	-0.23	7

图6 铜藻抗氧化系统PCA分析

Fig. 6 PCA analysis of antioxidant system in S. horneri

参考文献 36

[1]	BANKAJI I, CA ADOR I, SLEIMI N, 2015. Physiological and biochemical responses ofSuaeda fruticosa to cadmium and copper stresses: growth, nutrient uptake, antioxidant enzymes, phytochelatin, and glutathione levels[J]. Environmental Science and Pollution Research International, 22(17): 13058-13069. DOI URL
[2]	BASU A, MAHATA J, GUPTA S, et al., 2001. Genetic toxicology of a paradoxical human carcinogen, arsenic: a review[J]. Mutation Research, 488(2): 171-194. DOI URL
[3]	COOPER K L, KE J L, HUDSON L G, 2009. Enhanced ROS production and redox signaling with combined arsenite and UVA exposure: Contribution of NADPH oxidase[J]. Free Radical Biology and Medicine, 47(4): 381-388. DOI URL
[4]	FERRERI C, PANAGIOTAKI M, CHATGILIALOGLU C, 2007. Trans fatty acids in membranes: the free radical path[J]. Molecular Biotechnology, 37(1): 19-25. DOI URL
[5]	FLORA S J S, 2011. Arsenic-induced oxidative stress and its reversibility[J]. Free Radical Biology and Medicine, 51(2): 257-281. DOI URL
[6]	HEI T K, FILIPIC M, 2004. Role of oxidative damage in the genotoxicity of arsenic[J]. Free Radical Biology and Medicine, 37(5): 574-581. DOI URL
[7]	KOCH I, MCPHERSON K, SMITH P, et al., 2007. Arsenic bioaccessibility and speciation in clams and seaweed from a contaminated marine environment[J]. Marine Pollution Bulletin, 54(5): 586-594. DOI URL
[8]	LUNA A L, ACOSTA-SAAVEDRA L C, LOPEZ-CARRILLO L, et al., 2010. Arsenic alters monocyte superoxide anion and nitric oxide production in environmentally exposed children[J]. Toxicology and Applied Pharmacology, 245(2): 244-251. DOI URL
[9]	NAKAJIMA Y, ENDO Y, INOUE Y, et al., 2010. Ingestion of Hijiki seaweed and risk of arsenic poisoning[J]. Applied Organometallic Chemistry, 20(9): 557-564. DOI URL
[10]	PU F, REN X, 2014. Ascorbate levels and activities of enzymes related to the glutathione-ascorbate cycle in fruits of Chinese persimmon cultivars[J]. Horticulture, Environment and Biotechnology, 55(4): 315-321. DOI URL
[11]	RAO M V, AVANI G, 2004. Arsenic induced free radical toxicity in brain of mice[J]. Indian Journal of Experimental Biology, 42(5): 495-498.
[12]	SANITÀ DI TOPPI L, PAWLIK-SKOWROŃSKA B, VURRO E, et al., 2008. First and second line mechanisms of cadmium detoxification in the lichen photobiontTrebouxia impressa (Chlorophyta)[J]. Environmental Pollution, 151(2): 280-286. DOI URL
[13]	WOJAS S, CLEMENS S, SKŁODOWSKA A, et al., 2010. Arsenic response ofAtPCS1- andCePCS-expressing plants - Effects of external As(V) concentration on As-accumulation pattern and NPT metabolism[J]. Journal of Plant Physiology, 167(3): 169-175. DOI URL
[14]	陈贵, 胡文玉, 谢甫绨, 等, 1991. 提取植物体内MDA的溶剂及MDA作为衰老指标的探讨[J]. 植物生理学通讯, 27(1): 46-48.
	CHEN G, HU W Y, XIE P T, et al., 1991. Solvent for extracting malondialdehyde in plant as an index of senescence[J]. Plant Physiology Communication, 27(1): 46-48.
[15]	陈露, 嵇晶, 金佳颖, 等, 2020. 高效液相色谱-电感耦合等离子体质谱法测定海藻中5种砷形态[J]. 食品与发酵工业, 46(15): 270-275.
	CHEN L, JI J, JIN J Y, et al., 2020. Determination of five arsenic species in seaweed by HPLC-ICP-MS[J]. Food and Fermentation Industries, 46(15): 270-275.
[16]	陈天, 包宁颖, 杜崇宣, 等, 2020. 不同砷污染程度下香蒲生长与砷富集特征[J]. 浙江农业学报, 32(9): 1672-1682.
	CHEN T, BAO N Y, DU C X, et al., 2020. Growth and arsenic enrichment characteristics ofTypha angustifolia L. under different arsenic pollution levels[J]. Acta Agriculturae Zhejiangensis, 32(9): 1672-1682.
[17]	杜森, 张黎, 2019. 砷在海洋食物链中的生物放大潜力及发生机制探讨[J]. 生态毒理学报, 14(1): 54-66.
	DU S, ZHANG L, 2019. Biomagnification potential and the mechanisms of arsenic in marine food chains[J]. Asian Journal of Ecotoxicology, 14(1): 54-66.
[18]	段美红, 杨益, 李庆卫, 2020. 电导法结合Logistic方程鉴定嫁接繁殖梅花的抗寒性[J]. 中国园林, 36(S1): 67-70.
	DUAN M H, YANG Y, LI Q W, 2020. Identification of cold resistance based on conductance method and logistic equation in the top-grafting ofPrunus mume[J]. Chinese Landscape Architecture, 36(S1): 67-70.
[19]	樊香绒, 尹黎燕, 李伟, 等, 2013. 中国莲 (Nelumbo nucifera) 幼苗抗氧化系统对砷胁迫的响应[J]. 植物科学学报, 31(6): 570-575.
	FAN X R, YIN L Y, LI W, et al., 2013. Responses of the antioxidant system inNelumbo nucifera seedlings to arsenic toxicity[J]. Plant Science Journal, 31(6): 570-575. DOI URL
[20]	范宁波, 周俊学, 江凯, 等, 2020. 不同成熟期烤烟主脉中膜脂过氧化及其与衰老相关基因的关系探究[J]. 中国农业科技导报, DOI:10.13304/j.nykjdb.2019.0835. DOI
	FAN N B, ZHOU J X, JIANG K, et al., 2020. Study on menbrane lipid peroxidation and its relationship with senescence-related genes in main veins of flue-cured tobacoo at different maturity stages[J]. Journal of Agricultural Science and Technology, DOI:10.13304/j.nykjdb.2019.0835. DOI
[21]	胡家恕, 1995. 大豆种子萌发过程中砷毒害与膜脂过氧化作用[J]. 浙江农业大学学报, 21(4): 435-440.
	HU J S, 1995. The injury study of arsenic compound effect on germination of soybean and peroxidation of menbrane lipid[J]. Journal of Zhejiang Agricultural University, 21(4): 435-440.
[22]	胡立成, 张金怀, 2017. 乐清市水产品中的重金属及砷污染状况调查[J]. 中国卫生检验杂志, 27(13): 1937-1939.
	HU L C, ZHANG J H, 2017. Investigation of heavy metal and arsenic pollutionin aquatic products in Yueqing[J]. Chinese Journal of Health Laboratory Technology, 27(13): 1937-1939.
[23]	胡拥军, 王海娟, 王宏镔, 等, 2015. 砷胁迫下不同砷富集能力植物内源生长素与抗氧化酶的关系[J]. 生态学报, 35(10): 3214-3224.
	HU Y J, WANG H J, WANG H B, et al., 2015. The relationship between endogenous auxin and antioxidative enzymes in two plants with different arsenic-accumulative ability under arsenic stress[J]. Acta Ecologica Sinica, 35(10): 3214-3224.
[24]	马思思, 辛建攀, 陈宜栋, 等, 2020. 铜对梭鱼草叶片保护酶活性、抗氧化物质及非蛋白巯基肽含量的影响[J]. 草业科学, 37(3): 459-468.
	MA S S, XIN J P, CHEN Y D, et al., 2020. Effects of copper on antixoidant enzyme activities, antioxidant and non-protein thiol content inPontederia cordata’s leaves[J]. Pratacultural Science, 37(3): 459-468.
[25]	芮海云, 张兴兴, 庄凯, 等, 2018. 根非蛋白巯基化合物在箭舌豌豆两个品种镉耐性中的作用及机理[J]. 植物生理学报, 54(11): 1687-1694.
	RUI H Y, ZHANG X X, ZHUANG K, et al., 2018. The role of root non-protein thiols in cadmium tolerance of twoVicia sativa varieties and the underlying mechanism[J]. Plant Physiology Journal, 54(11): 1687-1694.
[26]	史静, 潘根兴, 2015. 外加镉对水稻镉吸收、亚细胞分布及非蛋白巯基含量的影响[J]. 生态环境学报, 24(5): 853-859.
	SHI J, PAN G X, 2015. Effects of Cd-spiking treatment on Cd accumulation, subcellular distribution and content of nonprotein thiols in rice[J]. Ecology and Environmental Sciences, 24(5): 853-859.
[27]	吴桂容, 洪华龙, 严重玲, 2016. S对As胁迫下桐花树幼苗巯基化合物含量的影响[J]. 厦门大学学报 (自然科学版),55(1): 55-59.
	WU G R, HONG H L, YAN C L, 2016. Influence of sulfur supply on thiols inAegiceras corniculation (L.) blanco under As stress[J]. Journal of Xiamen University (Natural Science), 55(1): 55-59.
[28]	杨承虎, 蔡景波, 张鹏, 等, 2017. 南麂列岛大型海藻重金属元素含量特征分析[J]. 海洋环境科学, 36(3): 372-378, 384.
	YANG C H, CAI J B, ZHANG P, et al., 2017. Determination of heavy metal contents in macroalgae from the Nanji Islands, China[J]. Marine Environmental Science, 36(3): 372-378, 384.
[29]	杨婉玲, 赖子尼, 曾艳艺, 等, 2015. 珠三角河网水、沉积物中As含量分布特征及污染评价[J]. 生态环境学报, 24(5): 831-837.
	YANG W L, LAI Z N, ZENG Y Y, et al., 2015. The distribution and contamination levels of arsenicinwater and sediment of the Pearl River Delta[J]. Ecology and Environmental Sciences, 24(5): 831-837.
[30]	叶鹏浩, 韩婷婷, 付贵权, 等, 2019. 半叶马尾藻对重金属镉胁迫的生理响应[J]. 南方水产科学, 15(5): 35-40.
	YE P H, HAN T T, FU G Q, et al., 2019. Physiological response ofSargassum hemiphyllum to cadmium stress[J]. South China Fisheries Science, 15(5): 35-40.
[31]	袁付红, 赵丽丽, 逄锦龙, 2019. 褐藻多酚提取、分离和抗氧化活性的研究进展[J]. 食品研究与开发, 40(18): 219-224.
	YUAN F H, ZHAO L L, PANG X L, 2019. Study on extraction, separation and antioxidant activity of phlorotannins[J]. Food Research and Development, 40(18): 219-224.
[32]	原海燕, 黄钢, 佟海英, 等, 2013. Cd胁迫下马蔺根和叶中非蛋白巯基肽含量的变化[J]. 生态环境学报, 22(7): 1214-1219.
	YUAN H Y, HUANG G, DONG H Y, et al., 2013. The change of non-protein thiol content in roots and leaves ofIris lactea var.chinensis under Cd stress[J]. Ecology and Environmental Sciences, 22(7): 1214-1219.
[33]	张玉, 张绵松, 史亚萍, 等, 2018. 铜藻活性组分多糖的体外抗氧化性研究[J]. 食品研究与开发, 39(6): 12-18.
	ZHANG Y, ZHANG M S, SHI Y P, et al., 2018. Comparison on Antioxidant Activity of Polysaccharide Fraction fromSargassum horneri in vitro [J]. Food Research and Development, 39(6): 12-18.
[34]	赵宁宁, 杜芮萍, 邱丹, 等, 2019. 蜈蚣草-玉米套作模式对玉米砷胁迫的缓解效应[J]. 生态环境学报, 28(5): 1021-1028.
	ZHAO N N, DU R P, QIU D, et al., 2019. Alleviating effects ofPteris vittata L.-maize intercropping system on arsenic stress in maize[J]. Ecology and Environmental Sciences, 28(5): 1021-1028.
[35]	郑丽杰, 缪晓冬, 韩威, 等, 2020. 铜藻主要化学成分分析及抗氧化活性评价[J]. 食品工业科技, 41(22): 232-239.
	ZHENG L J, MIAO X D, HAN W, et al., 2020. Analysis of main chemical components and evaluation of antioxidant activity ofSargassum horneri [J]. Science and Technology of Food Industry, 41(22): 232-239.
[36]	朱濛, 程楠楠, 杨如意, 等, 2020. 土壤-水环境中苯砷酸类化合物修复研究进展[J]. 生态环境学报, 29(7): 1475-1486.
	ZHU M, CHENG N N, YANG R Y, et al., 2020. Progress in researches on remediation of phenyl arsonic acid compounds in soil-water environment[J]. Ecology and Environmental Sciences, 29(7): 1475-1486.

无机砷短期胁迫对铜藻幼苗氧化损伤、抗氧化酶及抗氧化物的影响

Impacts of Short-term Inorganic Arsenic Stress on Oxidative Damage, Antioxidant Enzymes and Antioxidant in Germlings of Sargassum horneri

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 36

相关文章 5

编辑推荐

Metrics

本文评价

[1]	陈敏毅, 朱航海, 佘伟铎, 尹光彩, 黄祖照, 杨巧玲. 珠三角某遗留造船厂场地土壤重金属人体健康风险评估及源解析[J]. 生态环境学报, 2023, 32(4): 794-804.
[2]	陈金凤, 余世钦, 符加方, 徐国良, 于波, 赖晓群, 胡思源, 张开渠, 刘家华. 华南红层地貌区不同利用方式土壤质量特征及其影响因素——以南雄盆地为例[J]. 生态环境学报, 2022, 31(5): 918-930.
[3]	陈双双, 朱宁华, 周光益, 袁星明, 尚海, 王迤翾. 不同等级石漠化环境下人工乔木林的植被与土壤物理特征[J]. 生态环境学报, 2022, 31(1): 52-61.
[4]	孙文泰, 马明, 董铁, 牛军强, 尹晓宁, 刘兴禄. 西北旱地苹果细根分布及水力特征对长期覆膜的响应[J]. 生态环境学报, 2021, 30(7): 1375-1385.
[5]	张晋龙, 黄颖, 吴丽芳, 龚云辉, 刘云根, 王妍, 杨思林. 砷胁迫对狭叶香蒲生理生态及砷亚细胞分布的影响[J]. 生态环境学报, 2021, 30(5): 1042-1050.